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Hydroxyurea Can Be Used to Increase Mouse c-kit+Thy-1.1loLin-/loSca-1+ Hematopoietic Cell Number and Frequency in Cell Cycle In Vivo
Nobuko Uchida,
Annabelle M. Friera,
Dongping He,
Michael J. Reitsma,
Ann S. Tsukamoto, and
Irving L. Weissman
From SyStemix Inc, Palo Alto; and the Department of Pathology, Stanford School of Medicine, Stanford, CA.
The DNA synthesis inhibitor hydroxyurea (HU) was administered to determine whether it induces changes in the cell-cycle status of primitive hematopoietic stem cells (HSCs)/progenitors. Administration of HU to mice leads to bone marrow accumulation of c-kit+Thy-1.1loLin-/loSca-1+ (KTLS) cells in S/G2/M phases of the cell cycle. HU is a relatively nontoxic, reversible cell-cycle agent that can lead to approximately a threefold expansion of KTLS cells in vivo and approximately an eightfold increase in the number of KTLS cells in S/G2/M. HSCs in HU-treated mice have undiminished multilineage long-term and short-term clonal reconstitution activity.
Blood, Vol. 90 No. 11 (December 1), 1997:
pp. 4354-4362
© 1997 by The American Society of Hematology.
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