|
|
 Previous Article | Table of Contents | Next Article 
Human Herpesvirus 7 Induces CD4+ T-Cell Death by Two Distinct Mechanisms: Necrotic Lysis in Productively Infected Cells and Apoptosis in Uninfected or Nonproductively Infected Cells
Paola Secchiero,
Louis Flamand,
Davide Gibellini,
Elisabetta Falcieri,
Iole Robuffo,
Silvano Capitani,
Robert C. Gallo, and
Giorgio Zauli
From the Institute of Human Virology, University of Maryland at Baltimore, Baltimore, MD; the Institute of Human Anatomy, University of Ferrara, Ferrara, Italy; the Institute of Microbiology, University of Bologna, Bologna, Italy; the Institute of Anatomy and Physiology, University of Urbino, Urbino, Italy; the Institute of Normal and Pathological Cytomorphology of CNR, Institute of Research "Codivilla Putti," Bologna, Italy; and the Institute of Normal and Pathological Cytomorphology, CNR, Institute of Normal Human Morphology, Chieti, Italy.
We have investigated the cytopathic effects induced by the T-lymphotropic human herpesvirus 7 (HHV-7) on the CD4+ T-lymphoblastoid SupT1 cell line and primary CD4+ T lymphocytes. Acute in vitro HHV-7 infection induced (1) the formation of giant multinucleated syncytia, which eventually underwent necrotic lysis, and (2) single-cell apoptosis. Both cytopathic effects increased with the progression of infection and were blocked by phosphonoformic acid, a specific inhibitor of herpetic DNA polymerase. Using electron microscopy analysis of various samples, we found that all syncytia contained large amounts of virions and that most of them exhibited clear evidence of necrosis, whereas apoptosis was predominantly observed in single cells. Although empty viral capsids could be identified in the cytoplasm of approximately 25% of single cells exhibiting an apoptotic morphology, mature virions were hardly observed in these cells. In both coculture and cell-free HHV-7 infection experiments, a significant correlation was observed between the degree of single-cell apoptosis, evaluated by quantitative flow cytometry after propidium iodide staining, and the decrease in the total number of viable cells. Moreover, in cell-free infection experiments, apoptosis showed a positive correlation also with the viral load, monitored by quantitative HHV-7 DNA polymerase chain reaction. Thus, it appears that apoptosis occurred predominantly in uninfected bystander cells but not in productively HHV-7-infected cells.
Blood, Vol. 90 No. 11 (December 1), 1997:
pp. 4502-4512
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Perez, M. Inman, A. Doster, and C. Jones
Latency-Related Gene Encoded by Bovine Herpesvirus 1 Promotes Virus Growth and Reactivation from Latency in Tonsils of Infected Calves
J. Clin. Microbiol.,
January 1, 2005;
43(1):
393 - 401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. Stanziale, H. Petrowsky, P. S. Adusumilli, L. Ben-Porat, M. Gonen, and Y. Fong
Infection with Oncolytic Herpes Simplex Virus-1 Induces Apoptosis in Neighboring Human Cancer Cells: A Potential Target to Increase Anticancer Activity
Clin. Cancer Res.,
May 1, 2004;
10(9):
3225 - 3232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, P. Mirandola, D. Zella, C. Celeghini, A. Gonelli, M. Vitale, S. Capitani, and G. Zauli
Human herpesvirus 7 induces the functional up-regulation of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) coupled to TRAIL-R1 down-modulation in CD4+ T cells
Blood,
October 15, 2001;
98(8):
2474 - 2481.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Nargi-Aizenman and D. E. Griffin
Sindbis Virus-Induced Neuronal Death Is both Necrotic and Apoptotic and Is Ameliorated by N-Methyl-D-Aspartate Receptor Antagonists
J. Virol.,
August 1, 2001;
75(15):
7114 - 7121.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. Mirandola, P. Secchiero, S. Pierpaoli, G. Visani, L. Zamai, M. Vitale, S. Capitani, and G. Zauli
Infection of CD34+ hematopoietic progenitor cells by human herpesvirus 7 (HHV-7)
Blood,
July 1, 2000;
96(1):
126 - 131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, L. Bertolaso, L. Casareto, D. Gibellini, M. Vitale, K. Bemis, A. Aleotti, S. Capitani, G. Franchini, R. C. Gallo, et al.
Human Herpesvirus 7 Infection Induces Profound Cell Cycle Perturbations Coupled to Disregulation of cdc2 and Cyclin B and Polyploidization of CD4+ T Cells
Blood,
September 1, 1998;
92(5):
1685 - 1696.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
