A Chromosome 14q11/TCR
/
Specific Yeast Artificial Chromosome Improves the Detection Rate and Characterization of Chromosome Abnormalities in T-Lymphoproliferative Disorders
K.A. Rack,
F. Cornélis,
I. Radford-Weiss,
A. Bernheim,
C.J. Harrison,
O. Hermine,
M. Prieur,
M. Vekemans, and
E.A. Macintyre
From the Department of Hematology, Hôpital Necker Enfants Malades, Paris; INSERM U358, Hôpital Saint-Louis, Paris; Laboratoire de Cytogenetique, Hôpital Necker Enfants Malades, Paris; Laboratoire de Cytogenetique, Institut Gustave Roussy, Paris, France; Paterson Institute, Christie Hospital, Manchester, UK; CNRS URA 1461, Universite René Descartes, Paris V.
The rate of detection of chromosome abnormalities in T-cell proliferations is lower than that observed in B-cell malignancies. The former frequently involve the TCR
/
locus at chromosome band 14q11. We have identified a YAC encompassing 70% of the TCR/ locus, which has been used as a fluorescence in situ hybridization probe to detect chromosome rearrangements involving 14q11, both at metaphase and within interphase nuclei, in patients with a variety of T-lymphoproliferative disorders. Its use allowed detection of previously unsuspected TCR/ rearrangements in 4/13 (30%) immature T-lineage acute leukemias, including two t(10; 14) and 2 minor inversion 14s. It also clarified interpretation of complex chromosome 14 abnormalities in mature T-cell proliferations (T-prolymphocytic leukemia and ataxia telangiectasia). Use of this probe will aid the detection and characterization of abnormalities involving the TCR/ locus, particularly in cases with normal or complex karyotypes and in those proliferations for which mitoses are difficult to obtain.
Blood, Vol. 90 No. 3 (August 1), 1997:
pp. 1233-1240
© 1997 by The American Society of Hematology.
