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Tat-Human Immunodeficiency Virus-1 Induces Human Monocyte Chemotaxis by Activation of Vascular Endothelial Growth Factor Receptor-1
Stefania Mitola,
Silvano Sozzani,
Walter Luini,
Luca Primo,
Alessandro Borsatti,
Herbert Weich, and
Federico Bussolino
From the Department of Genetics, Biology and Medical Chemistry, Torino, Italy; the Mario Negri Institute for Pharmacological Research, Milano, Italy; and the Gesellschaft fuer Biotechologische Forschung, Braunschweig, Germany.
Human immunodeficiency virus-1 (HIV-1) Tat protein can be released by infected cells and activates mesenchymal cells. Among these, monocytes respond to Tat by migrating into tissues and releasing inflammatory mediators. In the present study, we have examined the molecular mechanism of monocyte activation by Tat, showing that this viral protein signals inside the cells through the tyrosine kinase receptor for vascular endothelial growth factor encoded by fms-like tyrosine kinase gene (VEGFR-1/Flt-1). Subnanomolar concentrations of Tat induced monocyte chemotaxis, which was inhibited by cell preincubation with vascular-endothelial growth factor-A (VEGF-A). This desensitisation was specific for VEGF-A, because it not was observed with FMLP. In addition, the soluble form of VEGFR-1 specifically inhibited polarization and migration induced by Tat and VEGF-A, thus confirming the common use of this receptor. Binding studies performed at equilibrium by using radiolabeled Tat showed that monocytes expressed a unique class of binding site, with a kd of approximately 0.2 nmol/L. The binding of radiolabeled Tat to monocyte surface and the cross-linking to a protein of 150 kD was inhibited specifically by an excess of cold Tat or VEGF-A. Western blot analysis with an antibody anti-VEGFR-1/Flt-1 performed on monocyte phosphoproteins immunoprecipitated by an monoclonal antibody antiphosphotyrosine showed that Tat induced a rapid phosphorylation in tyrosine residue of the 150-kD VEGFR-1/Flt-1. Taken together, these results suggest that biologic activities of HIV-1 Tat in human monocytes may, at least in part, be elicited by activation of VEGFR-1/Flt-1.
Blood, Vol. 90 No. 4 (August 15), 1997:
pp. 1365-1372
© 1997 by The American Society of Hematology.
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281(42):
31647 - 31658.
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|
|
|
|
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170(5):
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|
|
|
|
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S. Olsnes, O. Klingenberg, and A. Wiedlocha
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|
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|
|
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|
|
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|
|
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274(40):
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|
|
|
|
|
|
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94(5):
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|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
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93(11):
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|
|
|
|
|
|
|
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March 15, 1999;
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|
|
|
|
|
|
|
C. Ulich, A. Dunne, E. Parry, C. W. Hooker, R. B. Gaynor, and D. Harrich
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73(3):
2499 - 2508.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Shutt and D. Soll
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J. Cell Sci.,
January 11, 1999;
112(22):
3931 - 3941.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. D. Katsetos, J. E. Fincke, A. Legido, H. W. Lischner, J.-P. de Chadarevian, E. M. Kaye, C. D. Platsoucas, and E. L. Oleszak
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January 1, 1999;
6(1):
105 - 114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Albini, S. Ferrini, R. Benelli, S. Sforzini, D. Giunciuglio, M. G. Aluigi, A. E. I. Proudfoot, S. Alouani, T. N. C. Wells, G. Mariani, et al.
HIV-1 Tat protein mimicry of chemokines
PNAS,
October 27, 1998;
95(22):
13153 - 13158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Ganju, N. Munshi, B. C. Nair, Z.-Y. Liu, P. Gill, and J. E. Groopman
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J. Virol.,
July 1, 1998;
72(7):
6131 - 6137.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Albini, R. Benelli, D. Giunciuglio, T. Cai, G. Mariani, S. Ferrini, and D. M. Noonan
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J. Biol. Chem.,
June 26, 1998;
273(26):
15895 - 15900.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Shutt, L. Jenkins, E. Carolan, J Stapleton, K. Daniels, R. Kennedy, and D. Soll
T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber
J. Cell Sci.,
January 1, 1998;
111(1):
99 - 109.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Tyagi, M. Rusnati, M. Presta, and M. Giacca
Internalization of HIV-1 Tat Requires Cell Surface Heparan Sulfate Proteoglycans
J. Biol. Chem.,
January 26, 2001;
276(5):
3254 - 3261.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
