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Immunohistochemical Detection of the Epstein-Barr Virus-Encoded Latent Membrane Protein 2A in Hodgkin's Disease and Infectious Mononucleosis
G. Niedobitek,
E. Kremmer,
H. Herbst,
L. Whitehead,
C.W. Dawson,
E. Niedobitek,
C. von Ostau,
N. Rooney,
F.A. Grässer, and
L.S. Young
From the Institute for Cancer Studies and the Department of Pathology, University of Birmingham, Birmingham, UK; the Institut für Immunologie, GSF, München, Germany; the Institut für Pathologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany; the Department of Pathology, Royal United Hospital, Bath, UK; and the Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universitätskliniken des Saarlandes, Homburg, Germany.
We describe two new monoclonal antibodies specific for the Epstein-Barr virus (EBV)-encoded latent membrane protein 2A (LMP2A) that are suitable for the immunohistochemical analysis of routinely processed paraffin sections. These antibodies were applied to the immunohistochemical detection of LMP2A in Hodgkin's disease (HD). LMP2A-specific membrane staining was seen in the Hodgkin and Reed-Sternberg (HRS) cells of 22 of 42 (52%) EBV-positive HD cases, but not in 39 EBV-negative HD cases. In lymphoid tissues from patients with acute infectious mononucleosis (IM), interfollicular immunoblasts were shown to express LMP2A. This is the first demonstration of LMP2A protein expression at the single-cell level in EBV-associated lymphoproliferations in vivo. The detection of LMP2A protein expression in HD and IM is of importance in view of the proposed role of this protein for maintaining latent EBV infection and its possible contribution for EBV-associated transformation. Because LMP2A provides target epitopes for EBV-specific cytotoxic T cells, the expression of this protein in HRS cells has implications for the immunotherapeutic approaches to the treatment of HD.
Blood, Vol. 90 No. 4 (August 15), 1997:
pp. 1664-1672
© 1997 by The American Society of Hematology.
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