SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tam, S.-Y. Articles by Galli, S. J. Search for Related Content PubMed PubMed Citation Articles by Tam, S.-Y. Articles by Galli, S. J. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Expression of Functional TrkA Receptor Tyrosine Kinase in the HMC-1 Human Mast Cell Line and in Human Mast Cells See-Ying Tam, Mindy Tsai, Masao Yamaguchi, Koji Yano, Joseph H. Butterfield, and Stephen J. Galli From the Departments of Pathology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA; and the Division of Allergic Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, MN. Nerve growth factor (NGF ) can influence mast cell development and function in murine rodents by interacting with its receptors on mast cells. We now report the identification of mRNA transcripts of full-length tyrosine kinase-containing trkA, trkB, and trkC neurotrophin receptor genes in HMC-1 human mast cell leukemia cells. Although HMC-1 cells lacked p75 mRNA, they expressed transcripts for the exon-lacking splice variant of trkA (trkAI), truncated trkB (trkB.T1), and truncated trkC. By flow cytometry, HMC-1 cells exhibited expression of TrkA, TrkB, and TrkC receptor proteins containing full-length tyrosine kinase domains. NGF stimulation of HMC-1 cells induced tyrosine phosphorylation of TrkA protein, increased expression of the early response genes c-fos and NGF1-A, and activation of ERK-mitogen-activated protein (MAP) kinase, results which indicate that TrkA receptors in HMC-1 cells are fully functional. Highly purified populations of human lung mast cells expressed mRNAs for trkA, trkB and trkC, whereas preparations of human umbilical cord blood-derived mast cells expressed mRNAs for trkA and trkC, but not trkB. Moreover, preparations of human umbilical cord blood-derived immature mast cells not only expressed mRNA transcript and protein for TrkA, but exhibited significantly higher numbers of chymase-positive cells after the addition of NGF to their culture medium for 3 weeks. In addition, HMC-1 cells expressed mRNAs for NGF, brain-derived neurotrophic factor (BDNF ), and neurotrophin-3 (NT-3), the cognate ligands for TrkA, TrkB, and TrkC, whereas NGF and BDNF transcripts were detectable in human umbilical cord blood mast cell preparations. Taken together, our findings show that human mast cells express a functional TrkA receptor tyrosine kinase and indicate that NGF may be able to promote certain aspects of mast cell development and/or maturation in humans. Our studies also raise the possibility that human mast cells may represent a potential source for neurotrophins. Blood, Vol. 90 No. 5 (September 1), 1997: pp. 1807-1820 © 1997 by The American Society of Hematology. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Ma, N. Kambe, D. Wang, G. Shinoda, H. Fujino, K. Umeda, A. Fujisawa, L. Ma, H. Suemori, N. Nakatsuji, et al. Direct Development of Functionally Mature Tryptase/Chymase Double-Positive Connective Tissue-Type Mast Cells from Primate Embryonic Stem Cells Stem Cells, March 1, 2008; 26(3): 706 - 714. [Abstract] [Full Text] [PDF] J. Ahamed, R. T. Venkatesha, E. B. Thangam, and H. Ali C3a Enhances Nerve Growth Factor-Induced NFAT Activation and Chemokine Production in a Human Mast Cell Line, HMC-1 J. Immunol., June 1, 2004; 172(11): 6961 - 6968. [Abstract] [Full Text] [PDF] C. Nassenstein, A. Braun, V. J. Erpenbeck, M. Lommatzsch, S. Schmidt, N. Krug, W. Luttmann, H. Renz, and J. C. Virchow Jr. The Neurotrophins Nerve Growth Factor, Brain-derived Neurotrophic Factor, Neurotrophin-3, and Neurotrophin-4 Are Survival and Activation Factors for Eosinophils in Patients with Allergic Bronchial Asthma J. Exp. Med., August 4, 2003; 198(3): 455 - 467. [Abstract] [Full Text] [PDF] C. Olgart Hoglund, F. de Blay, J-P. Oster, C. Duvernelle, O. Kassel, G. Pauli, and N. Frossard Nerve growth factor levels and localisation in human asthmatic bronchi Eur. Respir. J., November 1, 2002; 20(5): 1110 - 1116. [Abstract] [Full Text] [PDF] G. Path, A. Braun, N. Meents, S. Kerzel, D. Quarcoo, U. Raap, G. W. Hoyle, W. A. Nockher, and H. Renz Augmentation of Allergic Early-Phase Reaction by Nerve Growth Factor Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 818 - 826. [Abstract] [Full Text] C. Olgart and N. Frossard Human lung fibroblasts secrete nerve growth factor: effect of inflammatory cytokines and glucocorticoids Eur. Respir. J., July 1, 2001; 18(1): 115 - 121. [Abstract] [Full Text] [PDF] J. Sawada, A. Itakura, A. Tanaka, T. Furusaka, and H. Matsuda Nerve growth factor functions as a chemoattractant for mast cells through both mitogen-activated protein kinase and phosphatidylinositol 3-kinase signaling pathways Blood, March 15, 2000; 95(6): 2052 - 2058. [Abstract] [Full Text] [PDF] L. O'Connell, J.-A. Hongo, L. G. Presta, and P. Tsoulfas TrkA Amino Acids Controlling Specificity for Nerve Growth Factor J. Biol. Chem., March 10, 2000; 275(11): 7870 - 7877. [Abstract] [Full Text] [PDF] C. A. Oskeritzian, Z. Wang, J. P. Kochan, M. Grimes, Z. Du, H.-W. Chang, S. Grant, and L. B. Schwartz Recombinant Human (rh)IL-4-Mediated Apoptosis and Recombinant Human IL-6-Mediated Protection of Recombinant Human Stem Cell Factor-Dependent Human Mast Cells Derived from Cord Blood Mononuclear Cell Progenitors J. Immunol., November 1, 1999; 163(9): 5105 - 5115. [Abstract] [Full Text] [PDF] A. Braun, M. Lommatzsch, A. Mannsfeldt, U. Neuhaus-Steinmetz, A. Fischer, N. Schnoy, G. R. Lewin, and H. Renz Cellular Sources of Enhanced Brain-Derived Neurotrophic Factor Production in a Mouse Model of Allergic Inflammation Am. J. Respir. Cell Mol. Biol., October 1, 1999; 21(4): 537 - 546. [Abstract] [Full Text] X.-Q. Shu and L. M. Mendell Neurotrophins and hyperalgesia PNAS, July 6, 1999; 96(14): 7693 - 7696. [Abstract] [Full Text] [PDF] J. S. Marshall, K. Gomi, M. G. Blennerhassett, and J. Bienenstock Nerve Growth Factor Modifies the Expression of Inflammatory Cytokines by Mast Cells Via a Prostanoid-Dependent Mechanism J. Immunol., April 1, 1999; 162(7): 4271 - 4276. [Abstract] [Full Text] [PDF] E. Labouyrie, P. Dubus, A. Groppi, F. X. Mahon, J. Ferrer, M. Parrens, J. Reiffers, A. de Mascarel, and J. P. Merlio Expression of Neurotrophins and their Receptors in Human Bone Marrow Am. J. Pathol., February 1, 1999; 154(2): 405 - 415. [Abstract] [Full Text] [PDF]

	Copyright © 1997 by American Society of Hematology         Online ISSN: 1528-0020