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Human Dendritic Cells Require Exogenous Interleukin-12-Inducing Factors to Direct the Development of Naive T-Helper Cells Toward the Th1 Phenotype
Catharien M.U. Hilkens,
Pawel Kalinski,
Mark de Boer, and
Martien L. Kapsenberg
From Academic Medical Center, University of Amsterdam, Department of Cell Biology & Histology, Amsterdam; and PanGenetics, Amsterdam, The Netherlands.
Dendritic cells (DC) are important initiators of specific primary immune responses because they are the only APC that can efficiently activate naive Th cells. DC have the capacity to produce interleukin-12 (IL-12), a cytokine that plays a pivotal role in the development of Th1-mediated cellular immune responses. The present study focuses on the conditions under which human DC produce bioactive IL-12 p70 and, consequently, direct the development of naive T helper (Th) cells toward the Th1 phenotype. Bacteria or bacterial compounds such as Staphylococcus aureus Cowan strain I (SAC) or lipopolysaccharide (LPS) induced substantial IL-12 levels in DC, which could be further upregulated by interferon- (IFN-), whereas induction of IL-12 production via CD40 ligation required IFN- as an obligatory, complementary signal. Also, activated naive Th cells were poor inducers of IL-12 production, unless exogenous IFN- was present, whereas activated memory Th cells were effective inducers of IL-12 production and did not require exogenous IFN-. Next, the cytokine profiles of matured Th cells that were primed by DC under different conditions were examined. DC promoted the development of naive Th cells into memory Th0 cells that produced both the type 1 cytokine IFN- and the type 2 cytokine IL-4. In contrast, after activation with SAC, DC efficiently directed the development of Th1 cells through the release of IL-12. An APC-independent Th cell maturation model, using either recombinant IL-12 or supernatants of SAC-activated DC and neutralizing anti-IL-12 antibodies, confirmed that DC-derived IL-12 was the major Th1 skewing factor. Together, these data indicate that the contact between DC and naive Th cells during the initiation of specific immune responses does not result in the efficient induction of IL-12 production and that, consequently, exogenous IL-12-inducing factors are required to promote primary Th1-mediated cellular immune responses.
Blood, Vol. 90 No. 5 (September 1), 1997:
pp. 1920-1926
© 1997 by The American Society of Hematology.
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15(2):
284 - 292.
[Abstract]
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|
|
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Z. Fan, X.-L. Huang, P. Kalinski, S. Young, and C. R. Rinaldo Jr.
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14(9):
1127 - 1137.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Haniffa, X.-N. Wang, U. Holtick, M. Rae, J. D. Isaacs, A. M. Dickinson, C. M. U. Hilkens, and M. P. Collin
Adult Human Fibroblasts Are Potent Immunoregulatory Cells and Functionally Equivalent to Mesenchymal Stem Cells
J. Immunol.,
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179(3):
1595 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takenaka, E. Safroneeva, Z. Xing, and J. Gauldie
Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics
J. Immunol.,
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178(12):
7984 - 7993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. R. Flores, K. A. Diggs, L. M. Tait, and P. A. Morel
IFN-{gamma} Negatively Regulates CpG-Induced IL-10 in Bone Marrow-Derived Dendritic Cells
J. Immunol.,
January 1, 2007;
178(1):
211 - 218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kitawaki, N. Kadowaki, N. Sugimoto, N. Kambe, T. Hori, Y. Miyachi, T. Nakahata, and T. Uchiyama
IgE-activated mast cells in combination with pro-inflammatory factors induce Th2-promoting dendritic cells
Int. Immunol.,
December 1, 2006;
18(12):
1789 - 1799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Bellone, A. Carbone, C. Smirne, T. Scirelli, A. Buffolino, A. Novarino, A. Stacchini, O. Bertetto, G. Palestro, C. Sorio, et al.
Cooperative induction of a tolerogenic dendritic cell phenotype by cytokines secreted by pancreatic carcinoma cells.
J. Immunol.,
September 1, 2006;
177(5):
3448 - 3460.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Macagno, M. Molteni, A. Rinaldi, F. Bertoni, A. Lanzavecchia, C. Rossetti, and F. Sallusto
A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression
J. Exp. Med.,
June 12, 2006;
203(6):
1481 - 1492.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Cao, P. Tan, C. H. Lee, H. Zhang, and J. Lu
A transforming growth factor-beta-induced protein stimulates endocytosis and is up-regulated in immature dendritic cells
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April 1, 2006;
107(7):
2777 - 2785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Cenci, E. Pericolini, A. Mencacci, S. Conti, W. Magliani, F. Bistoni, L. Polonelli, and A. Vecchiarelli
Modulation of phenotype and function of dendritic cells by a therapeutic synthetic killer peptide
J. Leukoc. Biol.,
January 1, 2006;
79(1):
40 - 45.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Rubio, T. K. Means, R. Chakraverty, J. Shaffer, Y. Fudaba, M. Chittenden, A. D. Luster, and M. Sykes
Maturation of human monocyte-derived dendritic cells (MoDCs) in the presence of prostaglandin E2 optimizes CD4 and CD8 T cell-mediated responses to protein antigens: role of PGE2 in chemokine and cytokine expression by MoDCs
Int. Immunol.,
December 1, 2005;
17(12):
1561 - 1572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Oki, C. Tomi, T. Yamamura, and S. Miyake
Preferential Th2 polarization by OCH is supported by incompetent NKT cell induction of CD40L and following production of inflammatory cytokines by bystander cells in vivo
Int. Immunol.,
December 1, 2005;
17(12):
1619 - 1629.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. B. Mailliard, S. M. Alber, H. Shen, S. C. Watkins, J. M. Kirkwood, R. B. Herberman, and P. Kalinski
IL-18-induced CD83+CCR7+ NK helper cells
J. Exp. Med.,
October 3, 2005;
202(7):
941 - 953.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Renkl, J. Wussler, T. Ahrens, K. Thoma, S. Kon, T. Uede, S. F. Martin, J. C. Simon, and J. M. Weiss
Osteopontin functionally activates dendritic cells and induces their differentiation toward a Th1-polarizing phenotype
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August 1, 2005;
106(3):
946 - 955.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Cumberbatch, K. Clelland, R. J. Dearman, and I. Kimber
Impact of Cutaneous IL-10 on Resident Epidermal Langerhans' Cells and the Development of Polarized Immune Responses
J. Immunol.,
July 1, 2005;
175(1):
43 - 50.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tabata, N. Kadowaki, T. Kitawaki, T. Shimaoka, S. Yonehara, O. Yoshie, and T. Uchiyama
Distribution and kinetics of SR-PSOX/CXCL16 and CXCR6 expression on human dendritic cell subsets and CD4+ T cells
J. Leukoc. Biol.,
May 1, 2005;
77(5):
777 - 786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Chagnon, S. Tanguay, O. L. Ozdal, M. Guan, Z. Z. Ozen, J.-S. Ripeau, M. Chevrette, M. M. Elhilali, and L. A. Thompson-Snipes
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February 1, 2005;
11(3):
1302 - 1311.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tavakoli, W. Schwerin, A. Rohwer, S. Hoffmann, S. Weyer, R. Weth, H. Meisel, H. Diepolder, M. Geissler, P. R. Galle, et al.
Phenotype and function of monocyte derived dendritic cells in chronic hepatitis B virus infection
J. Gen. Virol.,
October 1, 2004;
85(10):
2829 - 2836.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kikuchi, C. L. Hahn, S. Tanaka, S. E. Barbour, H. A. Schenkein, and J. G. Tew
Dendritic Cells Stimulated with Actinobacillus actinomycetemcomitans Elicit Rapid Gamma Interferon Responses by Natural Killer Cells
Infect. Immun.,
September 1, 2004;
72(9):
5089 - 5096.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rivas-Carvalho, M. A. Meraz-Rios, L. Santos-Argumedo, S. Bajana, G. Soldevila, M. E. Moreno-Garcia, and C. Sanchez-Torres
CD16+ human monocyte-derived dendritic cells matured with different and unrelated stimuli promote similar allogeneic Th2 responses: regulation by pro- and anti-inflammatory cytokines
Int. Immunol.,
September 1, 2004;
16(9):
1251 - 1263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Wassink, P. L. Vieira, H. H. Smits, G. A. Kingsbury, A. J. Coyle, M. L. Kapsenberg, and E. A. Wierenga
ICOS Expression by Activated Human Th Cells Is Enhanced by IL-12 and IL-23: Increased ICOS Expression Enhances the Effector Function of Both Th1 and Th2 Cells
J. Immunol.,
August 1, 2004;
173(3):
1779 - 1786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Matsushima, N. Yamada, H. Matsue, and S. Shimada
TLR3-, TLR7-, and TLR9-Mediated Production of Proinflammatory Cytokines and Chemokines from Murine Connective Tissue Type Skin-Derived Mast Cells but Not from Bone Marrow-Derived Mast Cells
J. Immunol.,
July 1, 2004;
173(1):
531 - 541.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Rouas, P. Lewalle, F. El Ouriaghli, B. Nowak, H. Duvillier, and P. Martiat
Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12
Int. Immunol.,
May 1, 2004;
16(5):
767 - 773.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. O'Donnell, B. G. Toelle, G. B. Marks, C. M. Hayden, I. A. Laing, J. K. Peat, J. Goldblatt, and P. N. Le Souef
Age-specific Relationship between CD14 and Atopy in a Cohort Assessed from Age 8 to 25 Years
Am. J. Respir. Crit. Care Med.,
March 1, 2004;
169(5):
615 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Athie-Morales, H. H. Smits, D. A. Cantrell, and C. M. U. Hilkens
Sustained IL-12 Signaling Is Required for Th1 Development
J. Immunol.,
January 1, 2004;
172(1):
61 - 69.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. P. Oliveira, G. M. A. C. Lima, M. T. Shio, P. J. M. Leenen, and I. A. Abrahamsohn
Immature macrophages derived from mouse bone marrow produce large amounts of IL-12p40 after LPS stimulation
J. Leukoc. Biol.,
November 1, 2003;
74(5):
857 - 867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Bedrosian, R. Mick, S. Xu, H. Nisenbaum, M. Faries, P. Zhang, P. A. Cohen, G. Koski, and B. J. Czerniecki
Intranodal Administration of Peptide-Pulsed Mature Dendritic Cell Vaccines Results in Superior CD8+ T-Cell Function in Melanoma Patients
J. Clin. Oncol.,
October 15, 2003;
21(20):
3826 - 3835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Miyazaki, H. Tsuda, M. Sakai, S. Hori, Y. Sasaki, T. Futatani, T. Miyawaki, and S. Saito
Predominance of Th2-promoting dendritic cells in early human pregnancy decidua
J. Leukoc. Biol.,
October 1, 2003;
74(4):
514 - 522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Smith, V. Patel, S. D. Seatter, M. R. Deehan, M. H. Brown, G. P. Brooke, H. S. Goodridge, C. J. Howard, K. P. Rigley, W. Harnett, et al.
A novel MyD-1 (SIRP-1{alpha}) signaling pathway that inhibits LPS-induced TNF{alpha} production by monocytes
Blood,
October 1, 2003;
102(7):
2532 - 2540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. T. Semnani, A. Y. Liu, H. Sabzevari, J. Kubofcik, J. Zhou, J. K. Gilden, and T. B. Nutman
Brugia malayi Microfilariae Induce Cell Death in Human Dendritic Cells, Inhibit Their Ability to Make IL-12 and IL-10, and Reduce Their Capacity to Activate CD4+ T Cells
J. Immunol.,
August 15, 2003;
171(4):
1950 - 1960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. L. Vieira, H. C. Heystek, J. Wormmeester, E. A. Wierenga, and M. L. Kapsenberg
Glatiramer Acetate (Copolymer-1, Copaxone) Promotes Th2 Cell Development and Increased IL-10 Production Through Modulation of Dendritic Cells
J. Immunol.,
May 1, 2003;
170(9):
4483 - 4488.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jinushi, T. Takehara, T. Kanto, T. Tatsumi, V. Groh, T. Spies, T. Miyagi, T. Suzuki, Y. Sasaki, and N. Hayashi
Critical Role of MHC Class I-Related Chain A and B Expression on IFN-{alpha}-Stimulated Dendritic Cells in NK Cell Activation: Impairment in Chronic Hepatitis C Virus Infection
J. Immunol.,
February 1, 2003;
170(3):
1249 - 1256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Leslie, M. S. Vincent, F. M. Spada, H. Das, M. Sugita, C. T. Morita, and M. B. Brenner
CD1-mediated {gamma}/{delta} T Cell Maturation of Dendritic Cells
J. Exp. Med.,
December 16, 2002;
196(12):
1575 - 1584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. P. Boonacker, E. A. Wierenga, H. H. Smits, and C. J.F. Van Noorden
CD26/DPPIV Signal Transduction Function, but Not Proteolytic Activity, Is Directly Related to Its Expression Level on Human Th1 and Th2 Cell Lines as Detected with Living Cell Cytochemistry
J. Histochem. Cytochem.,
September 1, 2002;
50(9):
1169 - 1177.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Lokshin, P. Kalinski, R. R. Sassi, R. B. Mailliard, J. Muller-Berghaus, W. J. Storkus, X. Peng, A. M. Marrangoni, R. P. Edwards, and E. Gorelik
Differential regulation of maturation and apoptosis of human monocyte-derived dendritic cells mediated by MHC class II
Int. Immunol.,
September 1, 2002;
14(9):
1027 - 1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. McDowell, M. Marovich, R. Lira, M. Braun, and D. Sacks
Leishmania Priming of Human Dendritic Cells for CD40 Ligand-Induced Interleukin-12p70 Secretion Is Strain and Species Dependent
Infect. Immun.,
August 1, 2002;
70(8):
3994 - 4001.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. B. Mailliard, S. Egawa, Q. Cai, A. Kalinska, S. N. Bykovskaya, M. T. Lotze, M. L. Kapsenberg, W. J. Storkus, and P. Kalinski
Complementary Dendritic Cell-activating Function of CD8+ and CD4+ T Cells: Helper Role of CD8+ T Cells in the Development of T Helper Type 1 Responses
J. Exp. Med.,
February 19, 2002;
195(4):
473 - 483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. F. Lipscomb and B. J. Masten
Dendritic Cells: Immune Regulators in Health and Disease
Physiol Rev,
January 1, 2002;
82(1):
97 - 130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. R. Christensen, H. Frokiar, and J. J. Pestka
Lactobacilli Differentially Modulate Expression of Cytokines and Maturation Surface Markers in Murine Dendritic Cells
J. Immunol.,
January 1, 2002;
168(1):
171 - 178.
[Abstract]
[Full Text]
| |
