|
|
 Previous Article | Table of Contents | Next Article 
Anti-CD43 Inhibits Monocyte-Endothelial Adhesion in Inflammation and Atherogenesis
Leslie M. McEvoy,
Mark A. Jutila,
Philip S. Tsao,
John P. Cooke, and
Eugene C. Butcher
From the Laboratory of Immunology and Vascular Biology, Department of Pathology and Department of Cardiovascular Medicine, Stanford University, Stanford; the Center for Molecular Biology and Medicine, Veterans Affairs Health Care System, Palo Alto, CA; and Veterinary Molecular Biology, Montana State University, Bozeman, MT.
Recruitment of blood monocytes into tissues is a central event in the inflammatory response and in atherogenesis. The mechanisms leading to monocyte adhesion and migration through endothelium are not completely defined. We recently reported that MAb L11, against the leukocyte sialomucin CD43, blocks T-lymphocyte binding to lymph node and Peyer's patch high endothelial venules (HEV) and inhibits T-cell extravasation from the blood into organized secondary lymphoid tissues. We have now assessed the ability of L11 to inhibit monocyte-endothelial (EC) interactions and trafficking. L11 blocks binding of WEHI78/24 cells, a murine monocytoid cell line, to inflamed lymph node HEV and inhibits recruitment of monocytes and neutrophils to thioglycollate-inflamed peritoneum. Because monocyte adhesion to the endothelium and diapedesis in lesion-prone regions of the vasculature is among the earliest events in atherogenesis, leading to formation of lipid-laden foam cells, the ability of L11 to block monocyte recognition of aortic endothelial cells was assessed in a novel ex vivo assay of monocyte binding to intact rabbit aortic endothelium. Cholesterol feeding of rabbits induces enhanced aortic adhesiveness for monocytes and WEHI78/24 monocytoid cells, and this adhesion is inhibited by L11. The inhibitory effect of L11 is additive with that of a cocktail of anti-L-selectin and anti- 4 and  2 integrin monoclonal antibodies. Thus, CD43 represents a novel target for manipulation of monocyte recruitment in inflammation and atherogenesis.
Blood, Vol. 90 No. 9 (November 1), 1997:
pp. 3587-3594
© 1997 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. Leon and C. Ardavin
Monocyte migration to inflamed skin and lymph nodes is differentially controlled by L-selectin and PSGL-1
Blood,
March 15, 2008;
111(6):
3126 - 3130.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Fuhlbrigge, S. L. King, R. Sackstein, and T. S. Kupper
CD43 is a ligand for E-selectin on CLA+ human T cells
Blood,
February 15, 2006;
107(4):
1421 - 1426.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lamagna, P. Meda, G. Mandicourt, J. Brown, R. J.C. Gilbert, E. Y. Jones, F. Kiefer, P. Ruga, B. A. Imhof, and M. Aurrand-Lions
Dual Interaction of JAM-C with JAM-B and {alpha}M{beta}2 Integrin: Function in Junctional Complexes and Leukocyte Adhesion
Mol. Biol. Cell,
October 1, 2005;
16(10):
4992 - 5003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. T. Bolick, S. Srinivasan, K. W. Kim, M. E. Hatley, J. J. Clemens, A. Whetzel, N. Ferger, T. L. Macdonald, M. D. Davis, P. S. Tsao, et al.
Sphingosine-1-Phosphate Prevents Tumor Necrosis Factor-{alpha}-Mediated Monocyte Adhesion to Aortic Endothelium in Mice
Arterioscler Thromb Vasc Biol,
May 1, 2005;
25(5):
976 - 981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. A. Muller
New Mechanisms and Pathways for Monocyte Recruitment
J. Exp. Med.,
November 5, 2001;
194(9):
F47 - F52.
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. T. Palframan, S. Jung, G. Cheng, W. Weninger, Y. Luo, M. Dorf, D. R. Littman, B. J. Rollins, H. Zweerink, A. Rot, et al.
Inflammatory Chemokine Transport and Presentation in HEV: A Remote Control Mechanism for Monocyte Recruitment to Lymph Nodes in Inflamed Tissues
J. Exp. Med.,
November 5, 2001;
194(9):
1361 - 1374.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Janatpour, S. Hudak, M. Sathe, J. D. Sedgwick, and L. M. McEvoy
Tumor Necrosis Factor-dependent Segmental Control of MIG Expression by High Endothelial Venules in Inflamed Lymph Nodes Regulates Monocyte Recruitment
J. Exp. Med.,
November 5, 2001;
194(9):
1375 - 1384.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fukuoka, K. Fukudome, Y. Yamashita, M. Tokushima, K. Miyake, and M. Kimoto
Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines
Blood,
December 15, 2000;
96(13):
4267 - 4275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. G. Johnson, A. Mikulowska, E. C. Butcher, L. M. McEvoy, and S. A. Michie
Anti-CD43 Monoclonal Antibody L11 Blocks Migration of T Cells to Inflamed Pancreatic Islets and Prevents Development of Diabetes in Nonobese Diabetic Mice
J. Immunol.,
November 15, 1999;
163(10):
5678 - 5685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Corinti, E. Fanales-Belasio, C. Albanesi, A. Cavani, P. Angelisova, and G. Girolomoni
Cross-Linking of Membrane CD43 Mediates Dendritic Cell Maturation
J. Immunol.,
June 1, 1999;
162(11):
6331 - 6336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. C. Woodman, B. Johnston, M. J. Hickey, D. Teoh, P. Reinhardt, B. Y. Poon, and P. Kubes
The Functional Paradox of CD43 in Leukocyte Recruitment: A Study Using CD43-deficient Mice
J. Exp. Med.,
December 7, 1998;
188(11):
2181 - 2186.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
