|
|
 Previous Article | Table of Contents | Next Article 
Platelet-Derived Interleukin-1 Induces Cytokine Production, but not
Proliferation of Human Vascular Smooth Muscle Cells
Harald Loppnow,
Rosita Bil,
Stephan Hirt,
Uwe Schönbeck,
Mona Herzberg,
Karl Werdan,
Ernst Theodor Rietschel,
Ernst Brandt, and
Hans-Dieter Flad
From Forschungszentrum Borstel, Zentrum für Medizin und
Biowissenschaften, Borstel; Martin-Luther-Universität
Halle-Wittenberg, Halle/Saale; and Klinik für Herz und
Gefäß-Chirurgie der Christian-Albrechts-Universität zu
Kiel, Kiel, Germany.
During vascular injury, such as observed in atherosclerosis,
restenosis, vasculitides, transplantation, or sepsis, vascular smooth
muscle cells (SMC) can be exposed to platelets or platelet products.
Under these conditions proliferation or cytokine production of SMC
stimulated by platelets or platelet products may contribute to
regulation of vascular pathogenesis. Thus, we investigated interleukin-6 (IL-6) and IL-8 production as well as proliferation of
SMC in response to platelets or platelet lysates. Platelets not already
preactivated by thrombin induced IL-6 (10- to 50-fold) or IL-8
production of unstimulated SMC in a cell number dependent fashion.
Preactivation of platelets with thrombin potently increased the
platelet-mediated IL-6 (50- to 1,000-fold) and IL-8 production of SMC.
Hirudin specifically inhibited the activation of platelets with
thrombin. Isolated platelets cultured in the absence of SMC did not
contain detectable IL-6 or IL-8. Prestimulation (4 hours) of SMC with
pathophysiologically relevant substances
(lipopolysaccharide [LPS], tumor necrosis factor- [TNF-], or
IL-1) further increased the platelet-induced cytokine production.
The platelet-derived SMC stimulatory activity was IL-1, since IL-1
receptor antagonist (IL-1-Ra) inhibited the platelet-induced cytokine
production of SMC. Anti-platelet-derived growth factor
(PDGF)-antibody did not further reduce this activity.
Thrombin itself stimulated expression of IL-6 and IL-8 to some degree
and induced IL-6 production of SMC synergistically with IL-1. Platelets
also induced proliferation of SMC, however, anti-PDGF antibodies,
rather than IL-1-Ra blocked this response. These data show that
platelet-derived IL-1 stimulates cytokine production of vascular smooth
muscle cells, indicating that platelet-derived IL-1 may contribute to
regulation of local pathogenesis in the vessel wall by activation of
the cytokine regulatory network.
Blood, Vol. 91 No. 1 (January 1), 1998:
pp. 134-141
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Chen, A. Frister, S. Wang, A. Ludwig, H. Behr, S. Pippig, B. Li, A. Simm, B. Hofmann, C. Pilowski, et al.
Interaction of vascular smooth muscle cells and monocytes by soluble factors synergistically enhances IL-6 and MCP-1 production
Am J Physiol Heart Circ Physiol,
April 1, 2009;
296(4):
H987 - H996.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Nold-Petry, M. F. Nold, J. A. Zepp, S.-H. Kim, N. F. Voelkel, and C. A. Dinarello
IL-32-dependent effects of IL-1{beta} on endothelial cell functions
PNAS,
March 10, 2009;
106(10):
3883 - 3888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. N. Shashkin, G. T. Brown, A. Ghosh, G. K. Marathe, and T. M. McIntyre
Lipopolysaccharide Is a Direct Agonist for Platelet RNA Splicing
J. Immunol.,
September 1, 2008;
181(5):
3495 - 3502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Zimmerman and A. S. Weyrich
Signal-Dependent Protein Synthesis by Activated Platelets: New Pathways to Altered Phenotype and Function
Arterioscler Thromb Vasc Biol,
March 1, 2008;
28(3):
s17 - s24.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Westphal, Li Chen, C. Pilowski, S. Koch, H. Ebelt, U. Muller-Werdan, K. Werdan, and H. Loppnow
Endotoxin-activated cultured neonatal rat cardiomyocytes express functional surface-associated interleukin-1{alpha}
Innate Immunity,
February 1, 2007;
13(1):
25 - 34.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L. Mandinov, K. L. Moodie, A. Mandinova, Z. Zhuang, F. Redican, D. Baklanov, V. Lindner, T. Maciag, M. Simons, and E. D. de Muinck
Inhibition of in-stent restenosis by oral copper chelation in porcine coronary arteries
Am J Physiol Heart Circ Physiol,
December 1, 2006;
291(6):
H2692 - H2697.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. L. Tavares, M. C. C. Sousa-e-Silva, M. L. Santoro, K. C. Barbaro, I. M. M. Rebecchi, and I. S. Sano-Martins
Changes in hematological, hemostatic and biochemical parameters induced experimentally in rabbits by Loxosceles gaucho spider venom
Human and Experimental Toxicology,
October 1, 2004;
23(10):
477 - 486.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. M. Opal
The nexus between systemic inflammation and disordered coagulation in sepsis
Innate Immunity,
April 1, 2004;
10(2):
125 - 129.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. S. Weyrich, S. M. Prescott, and G. A. Zimmerman
Platelets, Endothelial Cells, Inflammatory Chemokines, and Restenosis: Complex Signaling in the Vascular Play Book
Circulation,
September 17, 2002;
106(12):
1433 - 1435.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Brasier, A. Recinos III, and M. S. Eledrisi
Vascular Inflammation and the Renin-Angiotensin System
Arterioscler Thromb Vasc Biol,
August 1, 2002;
22(8):
1257 - 1266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. AUKRUST, T. WAeHRE, J. K. DAMAS, L. GULLESTAD, and N. O. SOLUM
Inflammatory role of platelets in acute coronary syndromes
Heart,
December 1, 2001;
86(6):
605 - 606.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Yoshida, S. G. Elner, Z.-M. Bian, S. L. Kunkel, N. W. Lukacs, and V. M. Elner
Thrombin Regulates Chemokine Induction during Human Retinal Pigment Epithelial Cell/Monocyte Interaction
Am. J. Pathol.,
September 1, 2001;
159(3):
1171 - 1180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Lindemann, N. D. Tolley, D. A. Dixon, T. M. McIntyre, S. M. Prescott, G. A. Zimmerman, and A. S. Weyrich
Activated platelets mediate inflammatory signaling by regulated interleukin 1{beta} synthesis
J. Cell Biol.,
August 6, 2001;
154(3):
485 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Ikeda, T. Ito, K. Shimada, O. Eickelberg, M. Roth, J. J. Rudiger, M. Tamm, A. P. Perruchoud, L.-H. Block, and R. Mussmann
Calcium Channel Blockers Activate the Interleukin-6 Gene Via the Transcription Factors NF-IL6 and NF-{kappa}B in Primary Human Vascular Smooth Muscle Cells Response
Circulation,
May 9, 2000;
101
(18):
e192 - e192.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Chandrasekar and J.-F. Tanguay
Platelets and restenosis
J. Am. Coll. Cardiol.,
March 1, 2000;
35(3):
555 - 562.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Stelter, H. Loppnow, R. Menzel, U. Grunwald, M. Bernheiden, R. S. Jack, A. J. Ulmer, and C. Schutt
Differential Impact of Substitution of Amino Acids 9-13 and 91-101 of Human CD14 on Soluble CD14-Dependent Activation of Cells by Lipopolysaccharide
J. Immunol.,
December 1, 1999;
163(11):
6035 - 6044.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. S Oemar
Is interleukin-1 beta a triggering factor for restenosis?
Cardiovasc Res,
October 1, 1999;
44(1):
17 - 19.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Han, M. S. Runge, and A. R. Brasier
Angiotensin II Induces Interleukin-6 Transcription in Vascular Smooth Muscle Cells Through Pleiotropic Activation of Nuclear Factor-{kappa}B Transcription Factors
Circ. Res.,
April 2, 1999;
84(6):
695 - 703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Goetze, X.-P. Xi, Y. Kawano, H. Kawano, E. Fleck, W. A. Hsueh, and R. E. Law
TNF-{alpha}–Induced Migration of Vascular Smooth Muscle Cells Is MAPK Dependent
Hypertension,
January 1, 1999;
33(1):
183 - 189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
