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A Novel P-Selectin Glycoprotein Ligand-1 Monoclonal Antibody
Recognizes an Epitope Within the Tyrosine Sulfate Motif of Human PSGL-1
and Blocks Recognition of Both P- and L-Selectin
Karen R. Snapp,
Han Ding,
Kristin Atkins,
Roger Warnke,
Francis W. Luscinskas, and
Geoffrey S. Kansas
From the Department of Microbiology/Immunology, Northwestern
University Medical School, Chicago, IL; Vascular Research Division, the
Department of Pathology, Brigham and Women's Hospital and Harvard
Medical School, Boston, MA; and the Department of Pathology, Stanford
University Medical School, Palo Alto, CA.
Interactions between P-selectin and P-selectin glycoprotein ligand-1
(PSGL-1) mediate the earliest "rolling" of leukocytes on the
lumenal surface of endothelial cells at sites of inflammation. Previously, PSGL-1 has been shown to be the primary mediator of interactions between neutrophils and P-selectin, but studies on the
ability of PSGL-1 to mediate interactions between P-selectin and other
subsets of leukocytes have yielded variable and conflicting results. A
novel IgG monoclonal antibody (MoAb) to human PSGL-1 was generated, and
the specificity of this MoAb was confirmed by both flow cytometric
analysis and Western blotting of cells transfected with human PSGL-1.
This newly developed MoAb, KPL1, inhibited interactions between
P-selectin expressing COS cells and either HL60 cells, neutrophils, or
lymphocytes. Furthermore, KPL1 completely inhibited interactions
between P-selectin and either purified CD4 T cells or neutrophils in a
flow assay under physiological conditions, but had no effect on
interactions of T cells or neutrophils with E-selectin. In addition,
KPL1 blocked interactions between lymphoid cells transfected with
L-selectin and COS cells expressing PSGL-1. The KPL1 epitope was mapped
to a site within a consensus tyrosine sulfation motif of PSGL-1, previously shown to be essential for interaction with P-selectin and
now shown to be essential for interaction with L-selectin, and to be
distinct from the epitope identified by the PL1 function blocking
anti-PSGL-1 MoAb. Two-color flow cytometry of normal leukocytes showed
that while natural killer (NK) cells (CD16+),
monocytes, CD4 and CD8 T cells, and  / and  / T cells were uniformly positive for PSGL-1, B cells expressed low levels of the KPL1
epitope. This low level of KPL1 staining was also observed immunohistologically in germinal centers, which had no detectable KPL1
staining, whereas T-cell areas (interfollicular region) were positive
for KPL1. Interestingly, plasma cells in situ and
interleukin-6-dependent myeloma cell lines were KPL1+.
Thus, PSGL-1 is expressed on essentially all blood neutrophils, NK
cells, B cells, T cells, and monocytes. Variation in tyrosine sulfation
during B-cell differentiation may affect the ability of B cells to
interact with P- and L-selectin.
Blood, Vol. 91 No. 1 (January 1), 1998:
pp. 154-164
© 1998 by The American Society of Hematology.
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|
|
|
|
|
|
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317(2):
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|
|
|
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|
|
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|
|
|
|
|
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T. Inoue, Y. Tsuzuki, K. Matsuzaki, H. Matsunaga, J. Miyazaki, R. Hokari, Y. Okada, A. Kawaguchi, S. Nagao, K. Itoh, et al.
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|
|
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|
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|
|
|
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|
|
|
|
|
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|
|
|
|
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|
|
|
|
|
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O. Dwir, A. Solomon, S. Mangan, G. S. Kansas, U. S. Schwarz, and R. Alon
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
C. B. Fieger, C. M. Sassetti, and S. D. Rosen
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27390 - 27398.
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|
|
|
|
|
|
|
L. Xia, V. Ramachandran, J. M. McDaniel, K. N. Nguyen, R. D. Cummings, and R. P. McEver
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January 15, 2003;
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|
|
|
|
|
|
|
B. Dang, S. Wiehler, and K. D. Patel
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J. Leukoc. Biol.,
October 1, 2002;
72(4):
702 - 710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Thatte, S. Ficarro, K. R. Snapp, M. K. Wild, D. Vestweber, D. F. Hunt, and K. F. Ley
Binding of function-blocking mAbs to mouse and human P-selectin glycoprotein ligand-1 peptides with and without tyrosine sulfation
J. Leukoc. Biol.,
September 1, 2002;
72(3):
470 - 477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kanamori, N. Kojima, K. Uchimura, T. Muramatsu, T. Tamatani, M. C. Berndt, G. S. Kansas, and R. Kannagi
Distinct Sulfation Requirements of Selectins Disclosed Using Cells That Support Rolling Mediated by All Three Selectins under Shear Flow. L-SELECTIN PREFERS CARBOHYDRATE 6-SULFATION TO TYROSINE SULFATION, WHEREAS P-SELECTIN DOES NOT
J. Biol. Chem.,
August 30, 2002;
277(36):
32578 - 32586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tarte, J. De Vos, T. Thykjaer, F. Zhan, G. Fiol, V. Costes, T. Reme, E. Legouffe, J.-F. Rossi, J. Shaughnessy Jr, et al.
Generation of polyclonal plasmablasts from peripheral blood B cells: a normal counterpart of malignant plasmablasts
Blood,
July 30, 2002;
100(4):
1113 - 1122.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. Snapp, C. E. Heitzig, and G. S. Kansas
Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton is essential for leukocyte rolling on P-selectin
Blood,
May 29, 2002;
99(12):
4494 - 4502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Fong, S. M. Alam, T. Imai, B. Haribabu, and D. D. Patel
CX3CR1 Tyrosine Sulfation Enhances Fractalkine-induced Cell Adhesion
J. Biol. Chem.,
May 24, 2002;
277(22):
19418 - 19423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Boyce, E. A. Mellor, B. Perkins, Y.-C. Lim, and F. W. Luscinskas
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Blood,
April 15, 2002;
99(8):
2890 - 2896.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. H. Underhill, H. A. Minges-Wols, J. L. Fornek, P. L. Witte, and G. S. Kansas
IgG plasma cells display a unique spectrum of leukocyte adhesion and homing molecules
Blood,
April 15, 2002;
99(8):
2905 - 2912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Dimitroff, J. Y. Lee, K. S. Schor, B. M. Sandmaier, and R. Sackstein
Differential L-Selectin Binding Activities of Human Hematopoietic Cell L-Selectin Ligands, HCELL and PSGL-1
J. Biol. Chem.,
December 7, 2001;
276(50):
47623 - 47631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Janowska-Wieczorek, M. Majka, J. Kijowski, M. Baj-Krzyworzeka, R. Reca, A. R. Turner, J. Ratajczak, S. G. Emerson, M. A. Kowalska, and M. Z. Ratajczak
Platelet-derived microparticles bind to hematopoietic stem/progenitor cells and enhance their engraftment
Blood,
November 15, 2001;
98(10):
3143 - 3149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-C. Lim, H. Xie, C. E. Come, S. I. Alexander, M. J. Grusby, A. H. Lichtman, and F. W. Luscinskas
IL-12, STAT4-Dependent Up-Regulation of CD4+ T Cell Core 2 {beta}-1,6-n-Acetylglucosaminyltransferase, an Enzyme Essential for Biosynthesis of P-Selectin Ligands
J. Immunol.,
October 15, 2001;
167(8):
4476 - 4484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. DiVietro, M. J. Smith, B. R. E. Smith, L. Petruzzelli, R. S. Larson, and M. B. Lawrence
Immobilized IL-8 Triggers Progressive Activation of Neutrophils Rolling In Vitro on P-Selectin and Intercellular Adhesion Molecule-1
J. Immunol.,
October 1, 2001;
167(7):
4017 - 4025.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Theoret, J.-G. Bienvenu, A. Kumar, and Y. Merhi
P-Selectin Antagonism with Recombinant P-Selectin Glycoprotein Ligand-1 (rPSGL-Ig) Inhibits Circulating Activated Platelet Binding to Neutrophils Induced by Damaged Arterial Surfaces
J. Pharmacol. Exp. Ther.,
August 1, 2001;
298(2):
658 - 664.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. White, G. H. Underhill, M. H. Kaplan, and G. S. Kansas
Cutting Edge: Differential Requirements for Stat4 in Expression of Glycosyltransferases Responsible for Selectin Ligand Formation in Th1 Cells
J. Immunol.,
July 15, 2001;
167(2):
628 - 631.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Evans, A. Leung, D. Hammer, and S. Simon
Chemically distinct transition states govern rapid dissociation of single L-selectin bonds under force
PNAS,
March 7, 2001;
(2001)
61324998.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. J. Dimitroff, J. Y. Lee, R. C. Fuhlbrigge, and R. Sackstein
A distinct glycoform of CD44 is an L-selectin ligand on human hematopoietic cells
PNAS,
November 22, 2000;
(2000)
250484797.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. Sackstein and C. J. Dimitroff
A hematopoietic cell L-selectin ligand that is distinct from PSGL-1 and displays N-glycan-dependent binding activity
Blood,
October 15, 2000;
96(8):
2765 - 2774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nomura, F. Okamae, M. Abe, M. Hosokawa, M. Yamaoka, T. Ohtani, S. Onishi, T. Matsuzaki, A. Teraoka, T. Ishida, et al.
Platelets Expressing P-Selectin and Platelet-Derived Microparticles in Stored Platelet Concentrates Bind to PSGL-1 on Filtrated Leukocytes
Clinical and Applied Thrombosis/Hemostasis,
October 1, 2000;
6(4):
213 - 221.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. J. Herron, C. M. Nelson, J. Larson, K. R. Snapp, G. S. Kansas, and J. L. Goodman
Intracellular Parasitism by the Human Granulocytic Ehrlichiosis Bacterium Through the P-Selectin Ligand, PSGL-1
Science,
June 2, 2000;
288(5471):
1653 - 1656.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. S. Frenette, C. V. Denis, L. Weiss, K. Jurk, S. Subbarao, B. Kehrel, J. H. Hartwig, D. Vestweber, and D. D. Wagner
P-Selectin Glycoprotein Ligand 1 (PSGL-1) Is Expressed on Platelets and Can Mediate Platelet-Endothelial Interactions In Vivo
J. Exp. Med.,
April 18, 2000;
191(8):
1413 - 1422.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Alonso-Lebrero, J. M. Serrador, C. Dominguez-Jimenez, O. Barreiro, A. Luque, M. A. del Pozo, K. Snapp, G. Kansas, R. Schwartz-Albiez, H. Furthmayr, et al.
Polarization and interaction of adhesion molecules P-selectin glycoprotein ligand 1 and intercellular adhesion molecule 3 with moesin and ezrin in myeloid cells
Blood,
April 1, 2000;
95(7):
2413 - 2419.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. R. Hentzen, S. Neelamegham, G. S. Kansas, J. A. Benanti, L. V. McIntire, C. W. Smith, and S. I. Simon
Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1
Blood,
February 1, 2000;
95(3):
911 - 920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Tu, P. G. Murphy, X. Li, and T. F. Tedder
L-Selectin Ligands Expressed by Human Leukocytes Are HECA-452 Antibody-Defined Carbohydrate Epitopes Preferentially Displayed by P-Selectin Glycoprotein Ligand-1
J. Immunol.,
November 1, 1999;
163(9):
5070 - 5078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Leppanen, P. Mehta, Y.-B. Ouyang, T. Ju, J. Helin, K. L. Moore, I. van Die, W. M. Canfield, R. P. McEver, and R. D. Cummings
A Novel Glycosulfopeptide Binds to P-selectin and Inhibits Leukocyte Adhesion to P-selectin
J. Biol. Chem.,
August 27, 1999;
274(35):
24838 - 24848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Ramos, Y. Huo, U. Jung, S. Ghosh, D. R. Manka, I. J. Sarembock, and K. Ley
Direct Demonstration of P-Selectin– and VCAM-1–Dependent Mononuclear Cell Rolling in Early Atherosclerotic Lesions of Apolipoprotein E–Deficient Mice
Circ. Res.,
June 11, 1999;
84(11):
1237 - 1244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bistrup, S. Bhakta, J. K. Lee, Y. Y. Belov, M. D. Gunn, F.-R. Zuo, C.-C. Huang, R. Kannagi, S. D. Rosen, and S. Hemmerich
Sulfotransferases of Two Specificities Function in the Reconstitution of High Endothelial Cell Ligands for L-selectin
J. Cell Biol.,
May 17, 1999;
145(4):
899 - 910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-C. Lim, L. Henault, A. J. Wagers, G. S. Kansas, F. W. Luscinskas, and A. H. Lichtman
Expression of Functional Selectin Ligands on Th Cells Is Differentially Regulated by IL-12 and IL-4
J. Immunol.,
March 15, 1999;
162(6):
3193 - 3201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Mukasa, T. Homma, T. Ohtsuki, O. Hosono, A. Souta, T. Kitamura, M. Fukuda, S. Watanabe, and C. Morimoto
Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells
Int. Immunol.,
February 1, 1999;
11(2):
259 - 268.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-C. Lim, K. Snapp, G. S. Kansas, R. Camphausen, H. Ding, and F. W. Luscinskas
Important Contributions of P-Selectin Glycoprotein Ligand-1-Mediated Secondary Capture to Human Monocyte Adhesion to P-Selectin, E-Selectin, and TNF-{alpha}-Activated Endothelium Under Flow In Vitro
J. Immunol.,
September 1, 1998;
161(5):
2501 - 2508.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. R. Snapp, R. Craig, M. Herron, R. D. Nelson, L. M. Stoolman, and G. S. Kansas
Dimerization of P-Selectin Glycoprotein Ligand-1 (PSGL-1) Required for Optimal Recognition of P-Selectin
J. Cell Biol.,
July 13, 1998;
142(1):
263 - 270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Wagers, L. M. Stoolman, R. Craig, R. N. Knibbs, and G. S. Kansas
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J. Immunol.,
May 15, 1998;
160(10):
5122 - 5129.
[Abstract]
[Full Text]
[PDF]
|
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|
|
|
P. Andre, O. Spertini, S. Guia, P. Rihet, F. Dignat-George, H. Brailly, J. Sampol, P. J. Anderson, and E. Vivier
Modification of P-selectin glycoprotein ligand-1 with a natural killer cell-restricted sulfated lactosamine creates an alternate ligand for L-selectin
PNAS,
March 28, 2000;
97(7):
3400 - 3405.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Evans, A. Leung, D. Hammer, and S. Simon
Chemically distinct transition states govern rapid dissociation of single L-selectin bonds under force
PNAS,
March 27, 2001;
98(7):
3784 - 3789.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Dimitroff, J. Y. Lee, R. C. Fuhlbrigge, and R. Sackstein
A distinct glycoform of CD44 is an L-selectin ligand on human hematopoietic cells
PNAS,
December 5, 2000;
97(25):
13841 - 13846.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
