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Impaired Ability of Bone Marrow Stromal Cells to
Support B-Lymphopoiesis With Age
Robert P. Stephan,
Colette R. Reilly, and
Pamela L. Witte
From the Departments of Cell Biology, Neurobiology, and Anatomy and
Microbiology and Immunology, Loyola University Chicago, Maywood, IL.
B-lymphopoiesis decreases with age. We studied how aging affects
bone marrow stromal cells, because they provide the growth factors and
cell contacts required for B-lymphopoiesis. No differences were noted
in the cell-surface phenotype of young and old primary-cultured stromal
cells. Fluorescence-activated cell sorter-purified
stromal cells from old mice were deficient in the ability to support
the proliferation of interleukin-7 (IL-7)-specific B-lymphoid cell lines. The kinetics of this response indicated that IL-7 was not immediately available from stromal cells of either age and was further
delayed on aged stromal cells. The levels of IL-7 protein within
stromal cells were equivalent between young and old animals, suggesting
that the production of IL-7 was not altered by aging. Negligible
amounts of IL-7 were found either freely secreted or in the
extracellular matrix of cultures of young and old marrow. Contact
between the lymphoid cells and the primary stromal cells was required
for detectable proliferation, suggesting that cell contact was required
for the release of IL-7. We propose that stromal cells regulate
B-lymphopoiesis by limiting the amount of IL-7 available to the
developing precursors. Therefore, we conclude that the age-related
decrease in the function of bone marrow stromal cells is related to the
impaired release of IL-7.
Blood, Vol. 91 No. 1 (January 1), 1998:
pp. 75-88
© 1998 by The American Society of Hematology.
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