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Myeloid Development Is Selectively Disrupted in PU.1 Null Mice
Karen L. Anderson,
Kent A. Smith,
Kris Conners,
Scott R. McKercher,
Richard A. Maki, and
Bruce E. Torbett
From The Burnham Institute, La Jolla, CA; and the Department of
Immunology, The Scripps Research Institute, La Jolla, CA.
The ets family transcription factor PU.1 is expressed in
monocytes/macrophages, neutrophils, mast cells, B cells, and early erythroblasts, but not in T cells. We have recently shown that PU.1
gene disruption results in mice with no detectable
monocytes/macrophages and B cells but T-cell development is retained.
Although neutrophil development occurred in these mice, it was delayed
and markedly reduced. We now proceed to demonstrate that PU.1 null
hematopoietic cells fail to proliferate or form colonies in response to
macrophage colony-stimulating factor (M-CSF), granulocyte CSF (G-CSF),
and granulocyte/macrophage CSF (GM-CSF). In contrast, PU.1 null cells did proliferate and form colonies in response to interleukin-3 (IL-3),
although the response was reduced as compared with control littermates.
Compared with control cells, PU.1 null cells had minimal expression of
G- and GM-CSF receptors and no detectable M-CSF receptors. The size of
individual myeloid colonies produced from PU.1 null primitive and
committed myeloid progenitors in the presence of IL-3, IL-6, and stem
cell factor (SCF) were reduced compared with controls. Under these
conditions, PU.1 null progenitors produced neutrophils but not
monocytes/macrophages. These observations suggest that PU.1 gene
disruption induces additional cell-autonomous effects that are
independent of the alterations in myeloid growth factor receptor
expression. Our results demonstrate that PU.1 gene disruption affects a
number of developmentally regulated hematopoietic processes that can,
at least in part, explain the changes in myeloid development and
reduction in myeloid and neutrophil expansion observed in PU.1 null
mice.
Blood, Vol. 91 No. 10 (May 15), 1998:
pp. 3702-3710
© 1998 by The American Society of Hematology.
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|
|
|
|
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|
|
|
|
|
|
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|
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|
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101(6):
2219 - 2226.
[Abstract]
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|
|
|
|
|
|
|
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1155 - 1163.
[Abstract]
[Full Text]
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|
|
|
|
|
|
|
J. Du, M. J. Stankiewicz, Y. Liu, Q. Xi, J. E. Schmitz, J. A. Lekstrom-Himes, and S. J. Ackerman
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277(45):
43481 - 43494.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Evans, S. Ariffin, A. Pierce, and A. D. Whetton
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October 16, 2002;
100(9):
3164 - 3174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
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Heterozygous PU.1 mutations are associated with acute myeloid leukemia
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July 18, 2002;
100(3):
998 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. Henkel, S. R. McKercher, and R. A. Maki
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14(7):
723 - 732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, Y. Okuno, P. Zhang, H. S. Radomska, H.-m. Chen, H. Iwasaki, K. Akashi, M. J. Klemsz, S. R. McKercher, R. A. Maki, et al.
Regulation of the PU.1 gene by distal elements
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November 15, 2001;
98(10):
2958 - 2965.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. B. Sykes and M. P. Kamps
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Blood,
October 15, 2001;
98(8):
2308 - 2318.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Alcamo, J. P. Mizgerd, B. H. Horwitz, R. Bronson, A. A. Beg, M. Scott, C. M. Doerschuk, R. O. Hynes, and D. Baltimore
Targeted Mutation of TNF Receptor I Rescues the RelA-Deficient Mouse and Reveals a Critical Role for NF-{kappa}B in Leukocyte Recruitment
J. Immunol.,
August 1, 2001;
167(3):
1592 - 1600.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Y. Chiang and J. G. Monroe
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J. Immunol.,
May 15, 2001;
166(10):
6091 - 6098.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Garrett-Sinha, R. Dahl, S. Rao, K. P. Barton, and M. C. Simon
PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1
Blood,
May 1, 2001;
97(9):
2908 - 2912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Zhang, X. Zhang, A. Iwama, C. Yu, K. A. Smith, B. U. Mueller, S. Narravula, B. E. Torbett, S. H. Orkin, and D. G. Tenen
PU.1 inhibits GATA-1 function and erythroid differentiation by blocking GATA-1 DNA binding
Blood,
October 15, 2000;
96(8):
2641 - 2648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Anderson, H. Perkin, C. D. Surh, S. Venturini, R. A. Maki, and B. E. Torbett
Transcription Factor PU.1 Is Necessary for Development of Thymic and Myeloid Progenitor-Derived Dendritic Cells
J. Immunol.,
February 15, 2000;
164(4):
1855 - 1861.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W Wood, M Turmaine, R Weber, V Camp, R. Maki, S. McKercher, and P Martin
Mesenchymal cells engulf and clear apoptotic footplate cells in macrophageless PU.1 null mouse embryos
Development,
January 12, 2000;
127(24):
5245 - 5252.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Rao, L. A. Garrett-Sinha, J. Yoon, and M. C. Simon
The Ets Factors PU.1 and Spi-B Regulate the Transcription in Vivo of P2Y10, a Lymphoid Restricted Heptahelical Receptor
J. Biol. Chem.,
November 26, 1999;
274(48):
34245 - 34252.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Anderson, K. A. Smith, H. Perkin, G. Hermanson, C.-G. Anderson, D. J. Jolly, R. A. Maki, and B. E. Torbett
PU.1 and the Granulocyte- and Macrophage Colony-Stimulating Factor Receptors Play Distinct Roles in Late-Stage Myeloid Cell Differentiation
Blood,
October 1, 1999;
94(7):
2310 - 2318.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-M. Kim, H.-S. Kang, S.-G. Paik, K.-H. Pyun, K. L. Anderson, B. E. Torbett, and I. Choi
Roles of IFN Consensus Sequence Binding Protein and PU.1 in Regulating IL-18 Gene Expression
J. Immunol.,
August 15, 1999;
163(4):
2000 - 2007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Pan, C. J. Hetherington, and D.-E. Zhang
CCAAT/Enhancer-binding Protein Activates the CD14 Promoter and Mediates Transforming Growth Factor beta Signaling in Monocyte Development
J. Biol. Chem.,
August 13, 1999;
274(33):
23242 - 23248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Lichanska, C. M. Browne, G. W. Henkel, K. M. Murphy, M. C. Ostrowski, S. R. McKercher, R. A. Maki, and D. A. Hume
Differentiation of the Mononuclear Phagocyte System During Mouse Embryogenesis: The Role of Transcription Factor PU.1
Blood,
July 1, 1999;
94(1):
127 - 138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. Henkel, S. R. McKercher, P. J.M. Leenen, and R. A. Maki
Commitment to the Monocytic Lineage Occurs in the Absence of the Transcription Factor PU.1
Blood,
May 1, 1999;
93(9):
2849 - 2858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Rao, A. Matsumura, J. Yoon, and M. C. Simon
SPI-B Activates Transcription via a Unique Proline, Serine, and Threonine Domain and Exhibits DNA Binding Affinity Differences from PU.1
J. Biol. Chem.,
April 16, 1999;
274(16):
11115 - 11124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. S. Egan, K. B. Lane, and V. L. Shepherd
PU.1 and USF Are Required for Macrophage-specific Mannose Receptor Promoter Activity
J. Biol. Chem.,
March 26, 1999;
274(13):
9098 - 9107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Behre, A. J. Whitmarsh, M. P. Coghlan, T. Hoang, C. L. Carpenter, D.-E. Zhang, R. J. Davis, and D. G. Tenen
c-Jun Is a JNK-independent Coactivator of the PU.1 Transcription Factor
J. Biol. Chem.,
February 19, 1999;
274(8):
4939 - 4946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Anderson, G Hernandez-Hoyos, R. Diamond, and E. Rothenberg
Precise developmental regulation of Ets family transcription factors during specification and commitment to the T cell lineage
Development,
January 6, 1999;
126(14):
3131 - 3148.
[Abstract]
[PDF]
|
|
|
|
|
|
|
N. C. Wilkinson and J. Navarro
PU.1 Regulates the CXCR1 Promoter
J. Biol. Chem.,
January 1, 1999;
274(1):
438 - 443.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Anderson, K. A. Smith, F. Pio, B. E. Torbett, and R. A. Maki
Neutrophils Deficient in PU.1 Do Not Terminally Differentiate or Become Functionally Competent
Blood,
September 1, 1998;
92(5):
1576 - 1585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Gaines, J. N. Geiger, G. Knudsen, D. Seshasayee, and D. M. Wojchowski
GATA-1- and FOG-dependent Activation of Megakaryocytic alpha IIB Gene Expression
J. Biol. Chem.,
October 27, 2000;
275(44):
34114 - 34121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Anderson, S. L. Nelson, H. B. Perkin, K. A. Smith, M. J. Klemsz, and B. E. Torbett
PU.1 Is a Lineage-specific Regulator of Tyrosine Phosphatase CD45
J. Biol. Chem.,
March 2, 2001;
276(10):
7637 - 7642.
[Abstract]
[Full Text]
[PDF]
|
|
|
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