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CD44 Selectively Associates With Active Src Family Protein Tyrosine
Kinases Lck and Fyn in Glycosphingolipid-Rich Plasma Membrane Domains
of Human Peripheral Blood Lymphocytes
Subburaj Ilangumaran,
Anne Briol, and
Daniel C. Hoessli
From the Department of Pathology, Centre Médical Universitaire,
Geneva, Switzerland.
CD44 is the major cell surface receptor for the extracellular matrix
glycosaminoglycan hyaluronan and is implicated in a variety of
biological events that include embryonic morphogenesis, lymphocyte recirculation, inflammation, and tumor metastasis. CD44 delivers activation signals to T lymphocytes, B lymphocytes, natural killer cells, polymorphonuclear leukocytes, and macrophages by stimulating protein tyrosine phosphorylation and calcium influx. The mechanism of
signal transduction via CD44 remains undefined, although CD44 was shown
to physically associate with intracellular protein tyrosine kinase Lck
in T lymphocytes. In the present report, we show that a significant
proportion of CD44 in human peripheral blood T lymphocytes and
endothelial cells is associated with low-density plasma membrane fractions that represent specialized plasma membrane domains enriched in glycosphingolipids and glycosylphosphatidylinositol (GPI)-anchored proteins. CD44 and the GPI-anchored CD59 do not appear to directly interact in the low-density membrane fractions. In human peripheral blood T lymphocytes, 20% to 30% of the Src family protein tyrosine kinases, Lck and Fyn, are recovered from these fractions.
CD44-associated protein kinase activity was selectively recovered from
the low-density membrane fractions, corresponding to
glycosphingolipid-rich plasma membrane microdomains. Reprecipitation of
the in vitro phosphorylated proteins showed that CD44 associates not
only with Lck but also with Fyn kinase in these membrane domains. Our
results suggest that cellular stimulation via CD44 may proceed through
the signaling machinery of glycosphingolipid-enriched plasma membrane
microdomains and, hence, depend on the functional integrity of such
domains.
Blood, Vol. 91 No. 10 (May 15), 1998:
pp. 3901-3908
© 1998 by The American Society of Hematology.
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|
|
|
|
|
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[Full Text]
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|
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|
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|
|
|
|
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|
|
|
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|
|
|
|
|
|
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|
|
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|
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|
|
|
|
|
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|
|
|
|
|
|
|
K. A. Fitzgerald, A. G. Bowie, B. S. Skeffington, and L. A. J. O'Neill
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[Abstract]
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[PDF]
|
|
|
|
|
|
|
Y. Yashiro-Ohtani, X.-Y. Zhou, K. Toyo-oka, X.-G. Tai, C.-S. Park, T. Hamaoka, R. Abe, K. Miyake, and H. Fujiwara
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164(3):
1251 - 1259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. B. Choi, C. K. Kim, and Y. Yun
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163(10):
5242 - 5249.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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November 15, 1999;
94(10):
3567 - 3575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kosugi, S.-i. Saitoh, S. Noda, K. Yasuda, F. Hayashi, M. Ogata, and T. Hamaoka
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Int. Immunol.,
September 1, 1999;
11(9):
1395 - 1401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Oliferenko, K. Paiha, T. Harder, V. Gerke, C. Schwarzler, H. Schwarz, H. Beug, U. Gunthert, and L. A. Huber
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J. Cell Biol.,
August 23, 1999;
146(4):
843 - 854.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Trkola, C. Gordon, J. Matthews, E. Maxwell, T. Ketas, L. Czaplewski, A. E. I. Proudfoot, and J. P. Moore
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J. Virol.,
August 1, 1999;
73(8):
6370 - 6379.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Ishiwatari-Hayasaka, T. Fujimoto, T. Osawa, T. Hirama, N. Toyama-Sorimachi, and M. Miyasaka
Requirements for Signal Delivery Through CD44: Analysis Using CD44-Fas Chimeric Proteins
J. Immunol.,
August 1, 1999;
163(3):
1258 - 1264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Dianzani, M. Bragardo, A. Tosti, L. Ruggeri, I. Volpi, M. Casucci, F. Bottarel, M. J. Feito, S. Bonissoni, and A. Velardi
CD44 signaling through p56lck involves lateral association with CD4 in human CD4+ T cells
Int. Immunol.,
July 1, 1999;
11(7):
1085 - 1092.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. P. Evanko, J. C. Angello, and T. N. Wight
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Arterioscler. Thromb. Vasc. Biol.,
April 1, 1999;
19(4):
1004 - 1013.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ilangumaran, S. Arni, G. van Echten-Deckert, B. Borisch, and D. C. Hoessli
Microdomain-dependent Regulation of Lck and Fyn Protein-Tyrosine Kinases in T Lymphocyte Plasma Membranes
Mol. Biol. Cell,
April 1, 1999;
10(4):
891 - 905.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. Li, N. Wong, M. D. Jabali, and P. Johnson
CD44-initiated Cell Spreading Induces Pyk2 Phosphorylation, Is Mediated by Src Family Kinases, and Is Negatively Regulated by CD45
J. Biol. Chem.,
July 27, 2001;
276(31):
28767 - 28773.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
