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The Effect of Apotransferrin on Iron Release From Caco-2 Cells,
an Intestinal Epithelial Cell Line
Xavier Alvarez-Hernandez,
Margaret Smith, and
Jonathan Glass
From the Section of Hematology/Oncology, Department of Medicine and
The Center for Excellence in Cancer Research, Louisiana State
University Medical Center, Shreveport, LA.
The Caco-2 cell line grown in bicameral chambers was used to study
the effect of transferrin in the basal chamber on the transepithelial transport of iron. We have shown that when iron was offered as 59Fe on the apical surface of the Caco-2 cells, transport
of 59Fe into the basal chamber was stimulated by 50 µmol/L apotransferrin. Here, we examined the effect on
59Fe transport of lower concentrations of apotransferrin,
as well as the effects on transport of ovo-, cobalt-, and
ferri-transferrin and of iron chelators with an affinity for iron
greater than that of transferrin. The stimulation of 59Fe
transport was more sensitive to the presence of apotransferrin with a
Km of 0.078 ± 0.008 µmol/L compared with
ferri-transferrin with a Km of 1.24 ± 0.39 µmol/L
(P < .006). 59Fe transport was less sensitive to
diethylenetriaminopenta-acetic acid (DTPA) than
apotransferrin with Kms of 1.52 ± 0.70. The chelator nitrilotriacetic acid (NTA) exhibited no stimulation of
59Fe transport. Analysis of laser scanning confocal
micrographs showed that apotransferrin labeled with Texas Red is
internalized by Caco-2 cells from the basal side and localizes in
distinct vesicles above the nucleus. The sensitivity of apotransferrin in stimulating Fe transport suggests a unique interaction of
apotransferrin with the basal surface of the intestinal epithelium.
Blood, Vol. 91 No. 10 (May 15), 1998:
pp. 3974-3979
© 1998 by The American Society of Hematology.
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