|
|
 Previous Article | Table of Contents | Next Article 
Anti-LFA-1 Blocking Antibodies Prevent Mobilization of
Hematopoietic Progenitor Cells Induced by Interleukin-8
Johannes F.M. Pruijt,
Yvette van Kooyk,
Carl G. Figdor,
Ivan J.D. Lindley,
Roel Willemze, and
Willem E. Fibbe
From the Laboratory of Experimental Hematology, the Department of
Hematology, Leiden University Medical Center, Leiden; the Department of
Tumor Immunology, University Hospital Nijmegen, The Netherlands; and
Novartis Forschungsinstitut, Vienna, Austria.
Previously, we have shown that interleukin (IL)-8 induces the rapid
(15 to 30 minutes) mobilization of hematopoietic progenitor cells (HPC)
in mice. Because integrins are essential for adhesion and
transendothelial migration of HPC, we studied the involvement of the
 2-integrin leukocyte function-associated antigen-1
(LFA-1) in IL-8-induced mobilization. After a single injection of
blocking anti-LFA-1 antibodies, no mobilization of colony-forming
cells was observed. In addition, when mice were pretreated with
anti-LFA-1 or saline and subsequently injected with 30 µg of IL-8,
mobilization of HPC was completely blocked. We showed that this was not
due to anti-LFA-1 antibodies affecting colony formation, as addition of anti-LFA-1 antibodies to colony cultures in semisolid medium had no
inhibitory activity. Also, anti-intercellular adhesion molecule
(ICAM)-1 antibodies, directed to the main ligand of LFA-1 significantly inhibited the IL-8-induced mobilization. Furthermore, IL-1-induced mobilization was significantly inhibited by anti-LFA-1 antibodies. Because LFA-1 is reported to be expressed on more differentiated HPC, it was considered that the IL-8-induced
mobilization of more primitive HPC would not be blocked by anti-LFA-1
antibodies. Transplantation of blood-derived mononuclear cells (MNC)
from IL-8-mobilized animals pretreated with anti-LFA-1 antibodies
protected only 25% of lethally irradiated recipient mice, whereas the
radioprotection rate of control mice transplanted with MNC derived from
IL-8-mobilized animals was 86% (P < .01). Anti-LFA-1
antibodies did not interfere with stem cell homing, as transplantation
of IL-8-mobilized blood MNC, incubated in vitro with these antibodies
resulted in 100% radioprotection. We conclude that anti-LFA-1
antibodies completely prevent the rapid mobilization of colony-forming
cells and of cells with radioprotective capacity induced by IL-8. These
results indicate a major role for the 2-integrin LFA-1
in the IL-8-induced mobilization of hematopoietic stem cells.
Blood, Vol. 91 No. 11 (June 1), 1998:
pp. 4099-4105
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. A. Schroeder, M. P. Rettig, P. Ramirez, and J. F. DiPersio
Optimal Mobilization of Normal and Leukemic Stem Cells via Modulation of Chemokine and Integrin Axes
Am. Assoc. Cancer Res. Educ. Book,
April 18, 2009;
2009(1):
15 - 22.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. van Pel, R. van Os, G. A. Velders, H. Hagoort, P. M. H. Heegaard, I. J. D. Lindley, R. Willemze, and W. E. Fibbe
Serpina1 is a potent inhibitor of IL-8-induced hematopoietic stem cell mobilization
PNAS,
January 31, 2006;
103(5):
1469 - 1474.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Wilson, M. J. Murphy, T. Oskarsson, K. Kaloulis, M. D. Bettess, G. M. Oser, A.-C. Pasche, C. Knabenhans, H. R. MacDonald, and A. Trumpp
c-Myc controls the balance between hematopoietic stem cell self-renewal and differentiation
Genes & Dev.,
November 15, 2004;
18(22):
2747 - 2763.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Carlo-Stella, M. Di Nicola, M. Magni, P. Longoni, M. Milanesi, C. Stucchi, L. Cleris, F. Formelli, and M. A. Gianni
Defibrotide in Combination with Granulocyte Colony-stimulating Factor Significantly Enhances the Mobilization of Primitive and Committed Peripheral Blood Progenitor Cells in Mice
Cancer Res.,
November 1, 2002;
62(21):
6152 - 6157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Velders, J. F. M. Pruijt, P. Verzaal, R. van Os, Y. van Kooyk, C. G. Figdor, E.-J. F. M. de Kruijf, R. Willemze, and W. E. Fibbe
Enhancement of G-CSF-induced stem cell mobilization by antibodies against the beta 2 integrins LFA-1 and Mac-1
Blood,
June 17, 2002;
100(1):
327 - 333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. M. Pruijt, P. Verzaal, R. van Os, E.-J. F. M. de Kruijf, M. L. J. van Schie, A. Mantovani, A. Vecchi, I. J. D. Lindley, R. Willemze, S. Starckx, et al.
Neutrophils are indispensable for hematopoietic stem cell mobilization induced by interleukin-8 in mice
PNAS,
April 30, 2002;
99(9):
6228 - 6233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Prosper and C. M. Verfaillie
Regulation of hematopoiesis through adhesion receptors
J. Leukoc. Biol.,
March 1, 2001;
69(3):
307 - 316.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Papayannopoulou, G. V. Priestley, B. Nakamoto, V. Zafiropoulos, L. M. Scott, and J. M. Harlan
Synergistic mobilization of hemopoietic progenitor cells using concurrent {beta}1 and {beta}2 integrin blockade or {beta}2-deficient mice
Blood,
March 1, 2001;
97(5):
1282 - 1288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peled, O. Kollet, T. Ponomaryov, I. Petit, S. Franitza, V. Grabovsky, M. M. Slav, A. Nagler, O. Lider, R. Alon, et al.
The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34+ cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice
Blood,
June 1, 2000;
95(11):
3289 - 3296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. J. Cotterell, C. R. Engwerda, and P. M. Kaye
Enhanced Hematopoietic Activity Accompanies Parasite Expansion in the Spleen and Bone Marrow of Mice Infected with Leishmania donovani
Infect. Immun.,
April 1, 2000;
68(4):
1840 - 1848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. M. Pruijt, W. E. Fibbe, L. Laterveer, R. A. Pieters, I. J. D. Lindley, L. Paemen, S. Masure, R. Willemze, and G. Opdenakker
Prevention of interleukin-8-induced mobilization of hematopoietic progenitor cells in rhesus monkeys by inhibitory antibodies against the Metalloproteinase gelatinase B (MMP-9)
PNAS,
September 14, 1999;
96(19):
10863 - 10868.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. B.H. Geijtenbeek, Y. van Kooyk, S. J. van Vliet, M. H. Renes, R. A.P. Raymakers, and C. G. Figdor
High Frequency of Adhesion Defects in B-Lineage Acute Lymphoblastic Leukemia
Blood,
July 15, 1999;
94(2):
754 - 764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F.M. Pruijt, Y. van Kooyk, C. G. Figdor, R. Willemze, and W. E. Fibbe
Murine Hematopoietic Progenitor Cells With Colony-Forming or Radioprotective Capacity Lack Expression of the beta 2-Integrin LFA-1
Blood,
January 1, 1999;
93(1):
107 - 112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
