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In Vitro Identification of Single
CD34+CD38 Cells With Both Lymphoid and
Myeloid Potential
Qian-Lin Hao,
Elzbieta M. Smogorzewska,
Lora W. Barsky, and
Gay M. Crooks
From the Division of Research Immunology/Bone Marrow Transplantation,
Childrens Hospital Los Angeles, Los Angeles, CA.
Human hematopoietic stem cells are pluripotent, ie, capable of
producing both lymphoid and myeloid progeny, and are therefore used for
transplantation and gene therapy. An in vitro culture system was
developed to study the multi-lineage developmental potential of a
candidate human hematopoietic stem cell population, CD34+CD38 cells.
CD34+CD38 cells cocultivated on the murine
stromal line S17 generated predominantly CD19+ B-cell
progenitors. Transfer of cells from S17 stroma to myeloid-specific conditions ("switch culture") showed that a fraction of the
immunophenotypically uncommitted CD19 cells generated on
S17 stroma had myeloid potential (defined by expression of CD33 and
generation of colony-forming unit-cells). Using the switch culture
system, single CD34+CD38 cells were
assessed for their lymphoid and myeloid potential. Nineteen of 50 (38%) clones generated from single
CD34+CD38 cells possessed both B-lymphoid
and myeloid potential. 94.7% of the
CD34+CD38 cells with lympho-myeloid
potential were late-proliferating (clonal appearance after 30 days),
demonstrating that pluripotentiality is detected significantly more
often in quiescent progenitors than in cytokine-responsive cells
(P = .00002). The S17/switch culture system permits the in
vitro assessment of the pluripotentiality of single human hematopoietic
cells.
Blood, Vol. 91 No. 11 (June 1), 1998:
pp. 4145-4151
© 1998 by The American Society of Hematology.
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