|
|
 Previous Article | Table of Contents | Next Article 
Interleukin-15 (IL-15) Can Replace the IL-2 Signal in IL-2-Dependent
Adult T-Cell Leukemia (ATL) Cell Lines: Expression of IL-15 Receptor
 on ATL Cells
Yasuaki Yamada,
Kazuyuki Sugawara,
Tomoko Hata,
Kazuto Tsuruta,
Ryozo Moriuchi,
Takahiro Maeda,
Sunao Atogami,
Ken Murata,
Kenshi Fujimoto,
Tomoko Kohno,
Kunihiro Tsukasaki,
Masao Tomonaga,
Yo-ichi Hirakata, and
Shimeru Kamihira
From the Departments of Laboratory Medicine, Bacteriology, and
Oncology, Nagasaki University School of Medicine; and the Department of
Hematology, Atomic Disease Institute, Nagasaki University School of
Medicine, Nagasaki, Japan.
Interleukin-15 receptor (IL-15R) and IL-2R have the same  and  chains, but IL-15R has a specific  chain distinct from
that of IL-2R, which is indispensable for the high affinity binding of IL-15. In the present study, we examined four IL-2-dependent adult
T-cell leukemia (ATL) cell lines for their IL-15R expression. All cell
lines bound IL-15, which was not inhibited by a 100-fold excess amount
of IL-2, proliferated in response to IL-15 to the same degree as to the
stimulation with IL-2, and were maintained without IL-2. The responses
to 1L-15 were inhibited by the antibodies against IL-2R or chains but was not by the IL-2R chain antibody. [125I]-IL-15 exhibited a single high-affinity binding
with an apparent kd of 0.17 nmol/L. Reverse
transcription-coupled polymerase chain reaction (RT-PCR) showed that
the cell lines had the mRNA of IL-15R . The cell lines also had
IL-15 mRNA. Despite the presence of IL-15 mRNA, the cell lines did not
secrete IL-15, and the culture supernatants of fresh ATL cells and
plasma from the patients did not contain a detectable amount of IL-15
with a few exceptional cases, although fresh ATL cells also responded
to IL-15. These results suggest that ATL cells have the complete form
of IL-15R and respond to IL-15. Such an IL-15-dependent cell
proliferation mechanism might be used in the development of ATL and for
the invasion and proliferation of ATL cells in the visceral organs.
Blood, Vol. 91 No. 11 (June 1), 1998:
pp. 4265-4272
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Chen, M. Petrus, B. R. Bryant, V. Phuc Nguyen, M. Stamer, C. K. Goldman, R. Bamford, J. C. Morris, J. E. Janik, and T. A. Waldmann
Induction of the IL-9 gene by HTLV-I Tax stimulates the spontaneous proliferation of primary adult T-cell leukemia cells by a paracrine mechanism
Blood,
May 15, 2008;
111(10):
5163 - 5172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. de Totero, R. Meazza, M. Capaia, M. Fabbi, B. Azzarone, E. Balleari, M. Gobbi, G. Cutrona, M. Ferrarini, and S. Ferrini
The opposite effects of IL-15 and IL-21 on CLL B cells correlate with differential activation of the JAK/STAT and ERK1/2 pathways
Blood,
January 15, 2008;
111(2):
517 - 524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Sasaki, I. Nishikata, T. Shiraga, E. Akamatsu, T. Fukami, T. Hidaka, Y. Kubuki, A. Okayama, K. Hamada, H. Okabe, et al.
Overexpression of a cell adhesion molecule, TSLC1, as a possible molecular marker for acute-type adult T-cell leukemia
Blood,
February 1, 2005;
105(3):
1204 - 1213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hayashibara, Y. Yamada, T. Miyanishi, H. Mori, T. Joh, T. Maeda, N. Mori, T. Maita, S. Kamihira, and M. Tomonaga
Vascular Endothelial Growth Factor and Cellular Chemotaxis: A Possible Autocrine Pathway in Adult T-Cell Leukemia Cell Invasion
Clin. Cancer Res.,
September 1, 2001;
7(9):
2719 - 2726.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. A. Fehniger and M. A. Caligiuri
Interleukin 15: biology and relevance to human disease
Blood,
January 1, 2001;
97(1):
14 - 32.
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Okada, T. Nakajima, M. Sanezumi, A. Ikuta, K. Yasuda, and H. Kanzaki
Progesterone Enhances Interleukin-15 Production in Human Endometrial Stromal Cells in Vitro
J. Clin. Endocrinol. Metab.,
December 1, 2000;
85(12):
4765 - 4770.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Mori, A. Ueda, S. Ikeda, Y. Yamasaki, Y. Yamada, M. Tomonaga, S. Morikawa, R. Geleziunas, T. Yoshimura, and N. Yamamoto
Human T-Cell Leukemia Virus Type I Tax Activates Transcription of the Human Monocyte Chemoattractant Protein-1 Gene through Two Nuclear Factor-{{kappa}}B Sites
Cancer Res.,
September 1, 2000;
60(17):
4939 - 4945.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Mori, M. Fujii, K. Iwai, S. Ikeda, Y. Yamasaki, T. Hata, Y. Yamada, Y. Tanaka, M. Tomonaga, and N. Yamamoto
Constitutive activation of transcription factor AP-1 in primary adult T-cell leukemia cells
Blood,
June 15, 2000;
95(12):
3915 - 3921.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Tsukada, Y. Toda, M. Misago, Y. Tanaka, P. E. Auron, and S. Eto
Constitutive activation of LIL-Stat in adult T-cell leukemia cells
Blood,
April 15, 2000;
95(8):
2715 - 2718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Tinhofer, I. Marschitz, T. Henn, A. Egle, and R. Greil
Expression of functional interleukin-15 receptor and autocrine production of interleukin-15 as mechanisms of tumor propagation in multiple myeloma
Blood,
January 15, 2000;
95(2):
610 - 618.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
