|
|
 Previous Article | Table of Contents | Next Article 
Transcriptional Regulation of vav, a Gene Expressed
Throughout the Hematopoietic Compartment
Sarah Ogilvy,
Andrew G. Elefanty,
Jane Visvader,
Mary L. Bath,
Alan W. Harris, and
Jerry M. Adams
From The Walter and Eliza Hall Institute of Medical Research,
Melbourne, Victoria, Australia.
The vav gene is expressed in all hematopoietic but few other
cell types. To explore its unusual compartment-wide regulation, we
cloned the murine gene, sequenced its promoter region, identified DNase
I hypersensitive (HS) sites in the chromatin, and tested their promoter
activity with a  -galactosidase (-gal) reporter gene in
cell lines and transgenic mice. Whereas fibroblasts had no HS sites, a
myeloid and an erythroid cell line contained five, located 0.2 kb
(HS1), 1.9 kb (HS2), and 3.6 kb (HS3) upstream from the transcription
start and 0.6 kb (HS4) and 10 kb (HS5) downstream. A vav DNA
fragment including HS1 promoted -gal expression in a myeloid but not
a fibroblast line. Expression in leukocytes of transgenic mice also
required HS2 and HS5. Only hematopoietic organs contained -gal, but
virtually all -gal+ cells were B or T
lymphocytes. Expression was always variegated (mosaic), and the
proportion of -gal+ cells declined with lymphoid
maturation and animal age. Thus, these vav regulatory elements
promoted hematopoietic-specific expression in vivo, at least in
lymphocytes, but the transgene was sporadically silenced. Maintaining
pan-hematopoietic expression may require additional vav
elements or an alternative reporter.
Blood, Vol. 91 No. 2 (January 15), 1998:
pp. 419-430
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Xing, T. H. Wanting, W. Zhao, J. Ma, S. Wang, X. Xu, Q. Li, X. Fu, M. Xu, and Z. J. Zhao
Transgenic expression of JAK2V617F causes myeloproliferative disorders in mice
Blood,
May 15, 2008;
111(10):
5109 - 5117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ogilvy, R. Ferreira, S. G. Piltz, J. M. Bowen, B. Gottgens, and A. R. Green
The SCL +40 Enhancer Targets the Midbrain Together with Primitive and Definitive Hematopoiesis and Is Regulated by SCL and GATA Proteins
Mol. Cell. Biol.,
October 15, 2007;
27(20):
7206 - 7219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Silberstein, M.-J. Sanchez, M. Socolovsky, Y. Liu, G. Hoffman, S. Kinston, S. Piltz, M. Bowen, L. Gambardella, A. R. Green, et al.
Transgenic Analysis of the Stem Cell Leukemia +19 Stem Cell Enhancer in Adult and Embryonic Hematopoietic and Endothelial Cells
Stem Cells,
September 1, 2005;
23(9):
1378 - 1388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Wiesner, J. M. Jones, D. E. Hasz, and D. A. Largaespada
Repressible transgenic model of NRAS oncogene-driven mast cell disease in the mouse
Blood,
August 1, 2005;
106(3):
1054 - 1062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Masters, K. R. Palmer, W. S. Stevenson, D. Metcalf, E. M. Viney, N. S. Sprigg, W. S. Alexander, N. A. Nicola, and S. E. Nicholson
Genetic Deletion of Murine SPRY Domain-Containing SOCS Box Protein 2 (SSB-2) Results in Very Mild Thrombocytopenia
Mol. Cell. Biol.,
July 1, 2005;
25(13):
5639 - 5647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-W. Lin, C. Slape, Z. Zhang, and P. D. Aplan
NUP98-HOXD13 transgenic mice develop a highly penetrant, severe myelodysplastic syndrome that progresses to acute leukemia
Blood,
July 1, 2005;
106(1):
287 - 295.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Delabesse, S. Ogilvy, M. A. Chapman, S. G. Piltz, B. Gottgens, and A. R. Green
Transcriptional Regulation of the SCL Locus: Identification of an Enhancer That Targets the Primitive Erythroid Lineage In Vivo
Mol. Cell. Biol.,
June 15, 2005;
25(12):
5215 - 5225.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Vassen, K. Fiolka, S. Mahlmann, and T. Möröy
Direct transcriptional repression of the genes encoding the zinc-finger proteins Gfi1b and Gfi1 by Gfi1b
Nucleic Acids Res.,
February 17, 2005;
33(3):
987 - 998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ogilvy, C. Louis-Dit-Sully, J. Cooper, R. L. Cassady, D. R. Alexander, and N. Holmes
Either of the CD45RB and CD45RO Isoforms Are Effective in Restoring T Cell, But Not B Cell, Development and Function in CD45-Null Mice
J. Immunol.,
August 15, 2003;
171(4):
1792 - 1800.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ogilvy, D. Metcalf, C. G. Print, M. L. Bath, A. W. Harris, and J. M. Adams
Constitutive Bcl-2 expression throughout the hematopoietic compartment affects multiple lineages and enhances progenitor cell survival
PNAS,
December 21, 1999;
96(26):
14943 - 14948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ogilvy, D. Metcalf, L. Gibson, M. L. Bath, A. W. Harris, and J. M. Adams
Promoter Elements of vav Drive Transgene Expression In Vivo Throughout the Hematopoietic Compartment
Blood,
September 15, 1999;
94(6):
1855 - 1863.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Elefanty, C. G. Begley, D. Metcalf, L. Barnett, F. Kontgen, and L. Robb
Characterization of hematopoietic progenitor cells that express the transcription factor SCL, using a lacZ "knock-in" strategy
PNAS,
September 29, 1998;
95(20):
11897 - 11902.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Nicholson, D. De Souza, L. J. Fabri, J. Corbin, T. A. Willson, J.-G. Zhang, A. Silva, M. Asimakis, A. Farley, A. D. Nash, et al.
Suppressor of cytokine signaling-3 preferentially binds to the SHP-2-binding site on the shared cytokine receptor subunit gp130
PNAS,
June 6, 2000;
97(12):
6493 - 6498.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
