|
|
 Previous Article | Table of Contents | Next Article 
 -Interferon Production in Peripheral Blood Mononuclear Cells and
Tumor Infiltrating Lymphocytes From Kaposi's Sarcoma Patients:
Correlation With the Presence of Human Herpesvirus-8 in Peripheral
Blood Mononuclear Cells and Lesional Macrophages
Maria Caterina Sirianni,
Laura Vincenzi,
Valeria Fiorelli,
Simone Topino,
Enrico Scala,
Stefania Uccini,
Antonio Angeloni,
Alberto Faggioni,
Decio Cerimele,
Francesca Cottoni,
Fernando Aiuti, and
Barbara Ensoli
From the Departments of Allergy and Clinical Immunology and
Experimental Medicine and Pathology, University of Rome "La
Sapienza," Rome; the Department of Dermatology, Catholic University
of Rome, Rome; the University of Sassari, Sassari; and the Laboratory
of Virology, Istituto Superiore di Sanità, Rome, Italy.
Evidence indicates that, at least in the early stage, Kaposi's
sarcoma (KS) is a cytokine-mediated disease and that it is consistently
associated with a novel herpesvirus termed human herpesvirus-8 (HHV-8).
To gain insights into the mechanisms by which cytokines and HHV-8 may
cooperate in disease pathogenesis, we examined the phenotype, the Th1
( -interferon [IFN]) and Th2 (interleukin-4 [IL-4]) cytokine
profile and the presence of HHV-8 in peripheral blood mononuclear cells
(PBMC), tumor-infiltrating lymphocytes (TIL), and spindle cell cultures
derived from skin lesions of patients affected by classical KS (C-KS)
and acquired immunodeficiency syndrome (AIDS)-associated KS (AIDS-KS).
TIL and spindle cell cultures were examined at day 0 or after culture in conditioned media from activated T cells (TCM) that contain the same
cytokines increased in KS tissues. No differences were found in the
immunophenotype of PBMC from C-KS patients versus controls, except for
AIDS-KS patients who showed a T-CD8+ expansion. However,
a preferential infiltration of T-CD8+ cells was found in
all KS lesions examined, which was maintained after culture of TIL in
TCM. IFN production was found in both PBMC and cultures derived from
all KS examined; some IL-4 positive supernatants were found only in
three AIDS-KS cases. Uninvolved skin did not show appreciable
lymphocyte infiltration or cytokine production. The culture conditions
of the lesional skin allowed also the appearance of adherent,
spindle-like cells bearing markers of tissue macrophages. Finally, most
or all of the PBMC, lesions, and macrophagic cell cultures from the
skin lesions were found to be positive for HHV-8 infection by nested
polymerase chain reaction (PCR). These findings indicate that patients
with KS express a Th1 phenotype with a prevalent IFN production,
likely accounted for by the local T-CD8+ infiltration. By
analogy with other viral infections (ie, Epstein-Barr virus), this
suggests that in loco recruitment of lymphoid cells and the subsequent
IFN production may be in response to or elicited by HHV-8 that was
found in both PBMC and macrophagic cell cultures from the lesions of
the same patients.
Blood, Vol. 91 No. 3 (February 1), 1998:
pp. 968-976
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Stebbing, A. Sanitt, A. Teague, T. Powles, M. Nelson, B. Gazzard, and M. Bower
Prognostic Significance of Immune Subset Measurement in Individuals With AIDS-Associated Kaposi's Sarcoma
J. Clin. Oncol.,
June 1, 2007;
25(16):
2230 - 2235.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. E. Brown, D. Fallin, I. Ruczinski, A. Hutchinson, B. Staats, F. Vitale, C. Lauria, D. Serraino, G. Rezza, G. Mbisa, et al.
Associations of Classic Kaposi Sarcoma with Common Variants in Genes that Modulate Host Immunity.
Cancer Epidemiol. Biomarkers Prev.,
May 1, 2006;
15(5):
926 - 934.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boccellino, G. Camussi, A. Giovane, L. Ferro, V. Calderaro, C. Balestrieri, and L. Quagliuolo
Platelet-Activating Factor Regulates Cadherin-Catenin Adhesion System Expression and {beta}-Catenin Phosphorylation during Kaposi's Sarcoma Cell Motility
Am. J. Pathol.,
May 1, 2005;
166(5):
1515 - 1522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Dourmishev, A. L. Dourmishev, D. Palmeri, R. A. Schwartz, and D. M. Lukac
Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis
Microbiol. Mol. Biol. Rev.,
June 1, 2003;
67(2):
175 - 212.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Cannon, N. J. Philpott, and E. Cesarman
The Kaposi's Sarcoma-Associated Herpesvirus G Protein-Coupled Receptor Has Broad Signaling Effects in Primary Effusion Lymphoma Cells
J. Virol.,
December 6, 2002;
77(1):
57 - 67.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Barillari and B. Ensoli
Angiogenic Effects of Extracellular Human Immunodeficiency Virus Type 1 Tat Protein and Its Role in the Pathogenesis of AIDS-Associated Kaposi's Sarcoma
Clin. Microbiol. Rev.,
April 1, 2002;
15(2):
310 - 326.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Toschi, G. Barillari, C. Sgadari, I. Bacigalupo, A. Cereseto, D. Carlei, C. Palladino, C. Zietz, P. Leone, M. Sturzl, et al.
Activation of Matrix-Metalloproteinase-2 and Membrane-Type-1-Matrix-Metalloproteinase in Endothelial Cells and Induction of Vascular Permeability In Vivo by Human Immunodeficiency Virus-1 Tat Protein and Basic Fibroblast Growth Factor
Mol. Biol. Cell,
October 1, 2001;
12(10):
2934 - 2946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Rimessi, A. Bonaccorsi, M. Sturzl, M. Fabris, E. Brocca-Cofano, A. Caputo, G. Melucci-Vigo, M. Falchi, A. Cafaro, E. Cassai, et al.
Transcription Pattern of Human Herpesvirus 8 Open Reading Frame K3 in Primary Effusion Lymphoma and Kaposi's Sarcoma
J. Virol.,
August 1, 2001;
75(15):
7161 - 7174.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Maeda, T. Niimi, S. Sato, H. Kawaguchi, Y. Sugiura, S. Mori, and R. Ueda
Human Herpesvirus 8 Is Not Associated With Sarcoidosis in Japanese Patients
Chest,
October 1, 2000;
118(4):
923 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Keller, E. J. Schattner, and E. Cesarman
Inhibition of NF-kappa B induces apoptosis of KSHV-infected primary effusion lymphoma cells
Blood,
October 1, 2000;
96(7):
2537 - 2542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Sgadari, E. Toschi, C. Palladino, G. Barillari, D. Carlei, A. Cereseto, C. Ciccolella, R. Yarchoan, P. Monini, M. Sturzl, et al.
Mechanism of Paclitaxel Activity in Kaposi's Sarcoma
J. Immunol.,
July 1, 2000;
165(1):
509 - 517.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Aboulafia
The Epidemiologic, Pathologic, and Clinical Features of AIDS-Associated Pulmonary Kaposi’s Sarcoma
Chest,
April 1, 2000;
117(4):
1128 - 1145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. L. nbecher, C. B. Foster, S. Zhu, D. Venzon, S. M. Steinberg, K. Wyvill, J. A. Metcalf, S. S. Cohen, J. Kovacs, R. Yarchoan, et al.
Variant genotypes of Fcgamma RIIIA influence the development of Kaposi's sarcoma in HIV-infected men
Blood,
April 1, 2000;
95(7):
2386 - 2390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Sturzl, C. Hohenadl, C. Zietz, E. Castanos-Velez, A. Wunderlich, G. Ascherl, P. Biberfeld, P. Monini, P. J. Browning, and B. Ensoli
Expression of K13/v-FLIP Gene of Human Herpesvirus 8 and Apoptosis in Kaposi's Sarcoma Spindle Cells
J Natl Cancer Inst,
October 20, 1999;
91(20):
1725 - 1733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Barillari, C. Sgadari, C. Palladino, R. Gendelman, A. Caputo, C. B. Morris, B. C. Nair, P. Markham, A. Nel, M. Sturzl, et al.
Inflammatory Cytokines Synergize with the HIV-1 Tat Protein to Promote Angiogenesis and Kaposi's Sarcoma Via Induction of Basic Fibroblast Growth Factor and the {alpha}v{beta}3 Integrin
J. Immunol.,
August 15, 1999;
163(4):
1929 - 1935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Barillari, C. Sgadari, V. Fiorelli, F. Samaniego, S. Colombini, V. Manzari, A. Modesti, B. C. Nair, A. Cafaro, M. Sturzl, et al.
The Tat Protein of Human Immunodeficiency Virus Type-1 Promotes Vascular Cell Growth and Locomotion by Engaging the alpha 5beta 1 and alpha vbeta 3 Integrins and by Mobilizing Sequestered Basic Fibroblast Growth Factor
Blood,
July 15, 1999;
94(2):
663 - 672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Mesri
Inflammatory Reactivation and Angiogenicity of Kaposi's Sarcoma-Associated Herpesvirus/HHV8: A Missing Link in the Pathogenesis of Acquired Immunodeficiency Syndrome-Associated Kaposi's Sarcoma
Blood,
June 15, 1999;
93(12):
4031 - 4033.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Monini, S. Colombini, M. Sturzl, D. Goletti, A. Cafaro, C. Sgadari, S. Butto, M. Franco, P. Leone, S. Fais, et al.
Reactivation and Persistence of Human Herpesvirus-8 Infection in B Cells and Monocytes by Th-1 Cytokines Increased in Kaposi's Sarcoma
Blood,
June 15, 1999;
93(12):
4044 - 4058.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Ascherl, C. Hohenadl, O. Schatz, E. Shumay, J. Bogner, L. Eckhart, E. Tschachler, P. Monini, B. Ensoli, and M. Sturzl
Infection With Human Immunodeficiency Virus-1 Increases Expression of Vascular Endothelial Cell Growth Factor in T Cells: Implications for Acquired Immunodeficiency Syndrome-Associated Vasculopathy
Blood,
June 15, 1999;
93(12):
4232 - 4241.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Monini, F. Carlini, M. Stürzl, P. Rimessi, F. Superti, M. Franco, G. Melucci-Vigo, A. Cafaro, D. Goletti, C. Sgadari, et al.
Alpha Interferon Inhibits Human Herpesvirus 8 (HHV-8) Reactivation in Primary Effusion Lymphoma Cells and Reduces HHV-8 Load in Cultured Peripheral Blood Mononuclear Cells
J. Virol.,
May 1, 1999;
73(5):
4029 - 4041.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Dupin, C. Fisher, P. Kellam, S. Ariad, M. Tulliez, N. Franck, E. van Marck, D. Salmon, I. Gorin, J.-P. Escande, et al.
Distribution of human herpesvirus-8 latently infected cells in Kaposi's sarcoma, multicentric Castleman's disease, and primary effusion lymphoma
PNAS,
April 13, 1999;
96(8):
4546 - 4551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Fiorelli, G. Barillari, E. Toschi, C. Sgadari, P. Monini, M. Sturzl, and B. Ensoli
IFN-{gamma} Induces Endothelial Cells to Proliferate and to Invade the Extracellular Matrix in Response to the HIV-1 Tat Protein: Implications for AIDS-Kaposi's Sarcoma Pathogenesis
J. Immunol.,
January 15, 1999;
162(2):
1165 - 1170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Murakami-Mori, S. Mori, and S. Nakamura
Endogenous Basic Fibroblast Growth Factor Is Essential for Cyclin E-CDK2 Activity in Multiple External Cytokine-Induced Proliferation of AIDS-Associated Kaposi's Sarcoma Cells: Dual Control of AIDS-Associated Kaposi's Sarcoma Cell Growth and Cyclin E-CDK2 Activity by Endogenous and External Signals
J. Immunol.,
August 15, 1998;
161(4):
1694 - 1704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
