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Stage-Specific Expression of Polycomb Group Genes in Human
Bone Marrow Cells
Julie Lessard,
Soheyl Baban, and
Guy Sauvageau
From the Laboratory of Molecular Genetics of Hemopoietic Stem Cells,
Clinical Research Institute of Montréal, Montréal,
Québec, Canada; the Département de Médecine,
Université de Montréal, Montréal, Québec,
Canada; and the Department of Experimental Medecine, McGill University,
Montréal, Québec, Canada.
Mammalian Polycomb group (Pc-G) genes, constituting some 5 subfamilies based on their identity to the Drosophila genes
Pc, Psc, ph, esc, and E(z), appear to play critical
roles in maintaining the transcriptional repression state of
Hox/HOM-C genes during development. Despite increasing evidence
of the important role of Hox genes in both normal hematopoiesis
and leukemic transformation, little is known about the expression and
possible function played by Pc-G genes in hematopoietic cells.
To address this, we first examined the expression of Pc genes
in purified CD34+ human bone marrow cells by reverse
transcriptase-polymerase chain reaction (RT-PCR), using
degenerate primers that specifically amplify the majority of Pc
genes. This analysis showed the expression of 8 different Pc
genes in CD34+ bone marrow cells, including
HP1Hs , HP1Hs , the heterochromatin
p25 protein, the human homologue of the murine M32 gene, and 4 novel members of this family. To assess whether Pc-G mRNA
levels change during differentiation of bone marrow cells, a
quantitative RT-PCR method was used to amplify the total cDNA
originating from three purified subpopulations of CD34+
bone marrow cells known to differ in their ability to grow in long-term
or semisolid cultures. In sharp contrast to Hox gene expression, which is highest in the most primitive bone marrow cells,
these studies show that the expression level of 8 of the 9 Pc-G
genes studied (ie, HP1Hs,
HP1Hs, M31, M32, M33, Mel-18,
Mph1/Rae-28, and ENX-1) markedly increases with
differentiation of bone marrow cells. Interestingly,
BMI-1 exhibits a strikingly different pattern of expression,
with high expression levels in primitive cells and very little
expression in mature CD34 cells. Together, these results
document for the first time that differentiation of human bone marrow
cells is accompanied by profound changes in Pc-G gene
expression levels.
Blood, Vol. 91 No. 4 (February 15), 1998:
pp. 1216-1224
© 1998 by The American Society of Hematology.
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