|
|
 Previous Article | Table of Contents | Next Article 
Stage-Specific Expression of Polycomb Group Genes in Human
Bone Marrow Cells
Julie Lessard,
Soheyl Baban, and
Guy Sauvageau
From the Laboratory of Molecular Genetics of Hemopoietic Stem Cells,
Clinical Research Institute of Montréal, Montréal,
Québec, Canada; the Département de Médecine,
Université de Montréal, Montréal, Québec,
Canada; and the Department of Experimental Medecine, McGill University,
Montréal, Québec, Canada.
Mammalian Polycomb group (Pc-G) genes, constituting some 5 subfamilies based on their identity to the Drosophila genes
Pc, Psc, ph, esc, and E(z), appear to play critical
roles in maintaining the transcriptional repression state of
Hox/HOM-C genes during development. Despite increasing evidence
of the important role of Hox genes in both normal hematopoiesis
and leukemic transformation, little is known about the expression and
possible function played by Pc-G genes in hematopoietic cells.
To address this, we first examined the expression of Pc genes
in purified CD34+ human bone marrow cells by reverse
transcriptase-polymerase chain reaction (RT-PCR), using
degenerate primers that specifically amplify the majority of Pc
genes. This analysis showed the expression of 8 different Pc
genes in CD34+ bone marrow cells, including
HP1Hs , HP1Hs , the heterochromatin
p25 protein, the human homologue of the murine M32 gene, and 4 novel members of this family. To assess whether Pc-G mRNA
levels change during differentiation of bone marrow cells, a
quantitative RT-PCR method was used to amplify the total cDNA
originating from three purified subpopulations of CD34+
bone marrow cells known to differ in their ability to grow in long-term
or semisolid cultures. In sharp contrast to Hox gene expression, which is highest in the most primitive bone marrow cells,
these studies show that the expression level of 8 of the 9 Pc-G
genes studied (ie, HP1Hs,
HP1Hs, M31, M32, M33, Mel-18,
Mph1/Rae-28, and ENX-1) markedly increases with
differentiation of bone marrow cells. Interestingly,
BMI-1 exhibits a strikingly different pattern of expression,
with high expression levels in primitive cells and very little
expression in mature CD34 cells. Together, these results
document for the first time that differentiation of human bone marrow
cells is accompanied by profound changes in Pc-G gene
expression levels.
Blood, Vol. 91 No. 4 (February 15), 1998:
pp. 1216-1224
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. V. Glinsky
"Stemness" Genomics Law Governs Clinical Behavior of Human Cancer: Implications for Decision Making in Disease Management
J. Clin. Oncol.,
June 10, 2008;
26(17):
2846 - 2853.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rizo, B. Dontje, E. Vellenga, G. de Haan, and J. J. Schuringa
Long-term maintenance of human hematopoietic stem/progenitor cells by expression of BMI1
Blood,
March 1, 2008;
111(5):
2621 - 2630.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ritou, M. Bai, and S. D. Georgatos
Variant-specific patterns and humoral regulation of HP1 proteins in human cells and tissues
J. Cell Sci.,
October 1, 2007;
120(19):
3425 - 3435.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Hosen, T. Yamane, M. Muijtjens, K. Pham, M. F. Clarke, and I. L. Weissman
Bmi-1-Green Fluorescent Protein-Knock-In Mice Reveal the Dynamic Regulation of Bmi-1 Expression in Normal and Leukemic Hematopoietic Cells
Stem Cells,
July 1, 2007;
25(7):
1635 - 1644.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Silva, J. M. Garcia, C. Pena, V. Garcia, G. Dominguez, D. Suarez, F. I. Camacho, R. Espinosa, M. Provencio, P. Espana, et al.
Implication of Polycomb Members Bmi-1, Mel-18, and Hpc-2 in the Regulation of p16INK4a, p14ARF, h-TERT, and c-Myc Expression in Primary Breast Carcinomas
Clin. Cancer Res.,
December 1, 2006;
12(23):
6929 - 6936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Chagraoui, S. L. Niessen, J. Lessard, S. Girard, P. Coulombe, M. Sauvageau, S. Meloche, and G. Sauvageau
E4F1: a novel candidate factor for mediating BMI1 function in primitive hematopoietic cells
Genes & Dev.,
August 1, 2006;
20(15):
2110 - 2120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Kamminga, L. V. Bystrykh, A. de Boer, S. Houwer, J. Douma, E. Weersing, B. Dontje, and G. de Haan
The Polycomb group gene Ezh2 prevents hematopoietic stem cell exhaustion
Blood,
March 1, 2006;
107(5):
2170 - 2179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Ravandi and Z. Estrov
Eradication of Leukemia Stem Cells as a New Goal of Therapy in Leukemia
Clin. Cancer Res.,
January 15, 2006;
12(2):
340 - 344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nowak, K. Kerl, D. Fehr, C. Kramps, C. Gessner, K. Killmer, B. Samans, B. Berwanger, H. Christiansen, and W. Lutz
BMI1 is a target gene of E2F-1 and is strongly expressed in primary neuroblastomas.
Nucleic Acids Res.,
January 1, 2006;
34(6):
1745 - 1754.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M Hinkins, J Huntriss, D Miller, and H M Picton
Expression of Polycomb-group genes in human ovarian follicles, oocytes and preimplantation embryos
Reproduction,
December 1, 2005;
130(6):
883 - 888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Kindler
Postnatal stem cell survival: does the niche, a rare harbor where to resist the ebb tide of differentiation, also provide lineage-specific instructions?
J. Leukoc. Biol.,
October 1, 2005;
78(4):
836 - 844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. Molofsky, S. He, M. Bydon, S. J. Morrison, and R. Pardal
Bmi-1 promotes neural stem cell self-renewal and neural development but not mouse growth and survival by repressing the p16Ink4a and p19Arf senescence pathways
Genes & Dev.,
June 15, 2005;
19(12):
1432 - 1437.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Miyazaki, H. Kawamoto, Y. Kato, M. Itoi, K. Miyazaki, K. Masuda, S. Tashiro, H. Ishihara, K. Igarashi, T. Amagai, et al.
Polycomb Group Gene mel-18 Regulates Early T Progenitor Expansion by Maintaining the Expression of Hes-1, a Target of the Notch Pathway
J. Immunol.,
March 1, 2005;
174(5):
2507 - 2516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bijl, M. Sauvageau, A. Thompson, and G. Sauvageau
High incidence of proviral integrations in the Hoxa locus in a new model of E2a-PBX1-induced B-cell leukemia
Genes & Dev.,
January 15, 2005;
19(2):
224 - 233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Raaphorst, M. Vermeer, E. Fieret, T. Blokzijl, D. Dukers, R. G.A.B. Sewalt, A. P. Otte, R. Willemze, and C. J.L.M. Meijer
Site-Specific Expression of Polycomb-Group Genes Encoding the HPC-HPH/PRC1 Complex in Clinically Defined Primary Nodal and Cutaneous Large B-Cell Lymphomas
Am. J. Pathol.,
February 1, 2004;
164(2):
533 - 542.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Boccuni, D. MacGrogan, J. M. Scandura, and S. D. Nimer
The Human L(3)MBT Polycomb Group Protein Is a Transcriptional Repressor and Interacts Physically and Functionally with TEL (ETV6)
J. Biol. Chem.,
April 18, 2003;
278(17):
15412 - 15420.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Mayotte, D.-C. Roy, J. Yao, E. Kroon, and G. Sauvageau
Oncogenic interaction between BCR-ABL and NUP98-HOXA9 demonstrated by the use of an in vitro purging culture system
Blood,
December 1, 2002;
100(12):
4177 - 4184.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Remillieux-Leschelle, P. Santamaria, and N. B. Randsholt
Regulation of Larval Hematopoiesis in Drosophila melanogaster: A Role for the multi sex combs Gene
Genetics,
November 1, 2002;
162(3):
1259 - 1274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Levine, A. Weiss, H. Erdjument-Bromage, Z. Shao, P. Tempst, and R. E. Kingston
The Core of the Polycomb Repressive Complex Is Compositionally and Functionally Conserved in Flies and Humans
Mol. Cell. Biol.,
September 1, 2002;
22(17):
6070 - 6078.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. van Kemenade, F. M. Raaphorst, T. Blokzijl, E. Fieret, K. M. Hamer, D. P. E. Satijn, A. P. Otte, and C. J. L. M. Meijer
Coexpression of BMI-1 and EZH2 polycomb-group proteins is associated with cycling cells and degree of malignancy in B-cell non-Hodgkin lymphoma
Blood,
June 15, 2001;
97(12):
3896 - 3901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Raaphorst, A. P. Otte, F. J. van Kemenade, T. Blokzijl, E. Fieret, K. M. Hamer, D. P. E. Satijn, and C. J. L. M. Meijer
Distinct BMI-1 and EZH2 Expression Patterns in Thymocytes and Mature T Cells Suggest a Role for Polycomb Genes in Human T Cell Differentiation
J. Immunol.,
May 15, 2001;
166(10):
5925 - 5934.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Beà, F. Tort, M. Pinyol, X. Puig, L. Hernández, S. Hernández, P. L. Fernández, M. van Lohuizen, D. Colomer, and E. Campo
BMI-1 Gene Amplification and Overexpression in Hematological Malignancies Occur Mainly in Mantle Cell Lymphomas
Cancer Res.,
March 1, 2001;
61(6):
2409 - 2412.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. M. Raaphorst, F. J. van Kemenade, T. Blokzijl, E. Fieret, K. M. Hamer, D. P. E. Satijn, A. P. Otte, and C. J. L. M. Meijer
Coexpression of BMI-1 and EZH2 Polycomb Group Genes in Reed-Sternberg Cells of Hodgkin's Disease
Am. J. Pathol.,
September 1, 2000;
157(3):
709 - 715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. M. Raaphorst, F. J. van Kemenade, E. Fieret, K. M. Hamer, D. P. E. Satijn, A. P. Otte, and C. J. L. M. Meijer
Cutting Edge: Polycomb Gene Expression Patterns Reflect Distinct B Cell Differentiation Stages in Human Germinal Centers
J. Immunol.,
January 1, 2000;
164(1):
1 - 4.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Hanson, J. L. Hess, B. D. Yu, P. Ernst, M. van Lohuizen, A. Berns, N. M. T. van der Lugt, C. S. Shashikant, F. H. Ruddle, M. Seto, et al.
Mammalian Trithorax and Polycomb-group homologues are antagonistic regulators of homeotic development
PNAS,
December 7, 1999;
96(25):
14372 - 14377.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lessard, A. Schumacher, U. Thorsteinsdottir, M. van Lohuizen, T. Magnuson, and G. Sauvageau
Functional antagonism of the Polycomb-Group genes eed and Bmi1 in hemopoietic cell proliferation
Genes & Dev.,
October 15, 1999;
13(20):
2691 - 2703.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Voncken, D Schweizer, L Aagaard, L Sattler, M. Jantsch, and M van Lohuizen
Chromatin-association of the Polycomb group protein BMI1 is cell cycle-regulated and correlates with its phosphorylation status
J. Cell Sci.,
January 12, 1999;
112(24):
4627 - 4639.
[Abstract]
[PDF]
|
|
|
|
|
|
|
D. P. E. Satijn and A. P. Otte
RING1 Interacts with Multiple Polycomb-Group Proteins and Displays Tumorigenic Activity
Mol. Cell. Biol.,
January 1, 1999;
19(1):
57 - 68.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
