|
|
 Previous Article | Table of Contents | Next Article 
Detailed Molecular Delineation of 13q14.3 Loss in B-Cell Chronic
Lymphocytic Leukemia
Martin M. Corcoran,
Omid Rasool,
Yie Liu,
Arati Iyengar,
Dan Grander,
Rachel E. Ibbotson,
Mats Merup,
Xiushan Wu,
Vadim Brodyansky,
Anne C. Gardiner,
Gunnar Juliusson,
Robert M. Chapman,
Ganka Ivanova,
Mary Tiller,
Gosta Gahrton,
Nick Yankovsky,
Eugene Zabarovsky,
David G. Oscier, and
Stefan Einhorn
From the Molecular Biology Laboratory, Royal Bournemouth General
Hospital, Bournemouth, UK; Radiumhemmet, Karolinska Hospital, Stockholm
Sweden; the Department of Medicine, Huddinge Hospital, Huddinge,
Sweden; the Laboratory of Genome Analysis, Institute of General
Genetics, Russian Academy of Science, Moscow, Russia; and the
Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm,
Sweden.
A region of chromosome 13q14.3, telomeric to the Retinoblastoma gene
RB-1 is frequently deleted in patients with B-cell chronic lymphocytic
leukemia (B-CLL). A cosmid and P1-derived artificial chromosome
(PAC) contig spanning over 600 kb has been constructed, which encompasses this locus. The contig clones have been used to order
a number of markers along the minimally deleted region and to localize
a series of CpG islands corresponding to possible candidate genes. A
novel polymorphic dinucleotide repeat, 6E3.2, present in one of the
ordered cosmid clones has been isolated for use in deletion mapping
studies of patient DNA. Leukemic samples from 229 CLL patients have
been screened for loss of heterozygosity using microsatellite markers
and analyzed for hemizygous and homozygous deletions by Southern blot
techniques using genomic probes selected from cosmids across the
region. Hemizygous deletions were found in 31% of cases with an
additional 10% showing homozygous loss. The use of these probes has
defined the commonly deleted area to less than 130 kb, centromeric to
the locus D13S272.
Blood, Vol. 91 No. 4 (February 15), 1998:
pp. 1382-1390
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Ng, O. Toure, M.-H. Wei, D. C. Arthur, F. Abbasi, L. Fontaine, G. E. Marti, J. F. Fraumeni Jr, L. R. Goldin, N. Caporaso, et al.
Identification of a novel chromosome region, 13q21.33-q22.2, for susceptibility genes in familial chronic lymphocytic leukemia
Blood,
February 1, 2007;
109(3):
916 - 925.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Haslinger, N. Schweifer, S. Stilgenbauer, H. Dohner, P. Lichter, N. Kraut, C. Stratowa, and R. Abseher
Microarray Gene Expression Profiling of B-Cell Chronic Lymphocytic Leukemia Subgroups Defined by Genomic Aberrations and VH Mutation Status
J. Clin. Oncol.,
October 1, 2004;
22(19):
3937 - 3949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kohlhammer, C. Schwaenen, S. Wessendorf, K. Holzmann, H. A. Kestler, D. Kienle, T. F. E. Barth, P. Moller, G. Ott, J. Kalla, et al.
Genomic DNA-chip hybridization in t(11;14)-positive mantle cell lymphomas shows a high frequency of aberrations and allows a refined characterization of consensus regions
Blood,
August 1, 2004;
104(3):
795 - 801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Calin, C. D. Dumitru, M. Shimizu, R. Bichi, S. Zupo, E. Noch, H. Aldler, S. Rattan, M. Keating, K. Rai, et al.
Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia
PNAS,
November 26, 2002;
99(24):
15524 - 15529.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Oscier, A. C. Gardiner, S. J. Mould, S. Glide, Z. A. Davis, R. E. Ibbotson, M. M. Corcoran, R. M. Chapman, P. W. Thomas, J. A. Copplestone, et al.
Multivariate analysis of prognostic factors in CLL: clinical stage, IGVH gene mutational status, and loss or mutation of the p53 gene are independent prognostic factors
Blood,
July 30, 2002;
100(4):
1177 - 1184.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Mertens, S. Wolf, P. Schroeter, C. Schaffner, H. Dohner, S. Stilgenbauer, and P. Lichter
Down-regulation of candidate tumor suppressor genes within chromosome band 13q14.3 is independent of the DNA methylation pattern in B-cell chronic lymphocytic leukemia
Blood,
May 13, 2002;
99(11):
4116 - 4121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Klein, Y. Tu, G. A. Stolovitzky, M. Mattioli, G. Cattoretti, H. Husson, A. Freedman, G. Inghirami, L. Cro, L. Baldini, et al.
Gene Expression Profiling of B Cell Chronic Lymphocytic Leukemia Reveals a Homogeneous Phenotype Related to Memory B Cells
J. Exp. Med.,
December 3, 2001;
194(11):
1625 - 1638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bullrich, H. Fujii, G. Calin, H. Mabuchi, M. Negrini, Y. Pekarsky, L. Rassenti, H. Alder, J. C. Reed, M. J. Keating, et al.
Characterization of the 13q14 Tumor Suppressor Locus in CLL: Identification of ALT1, an Alternative Splice Variant of the LEU2 Gene
Cancer Res.,
September 1, 2001;
61(18):
6640 - 6648.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mabuchi, H. Fujii, G. Calin, H. Alder, M. Negrini, L. Rassenti, T. J. Kipps, F. Bullrich, and C. M. Croce
Cloning and Characterization of CLLD6, CLLD7, and CLLD8, Novel Candidate Genes for Leukemogenesis at Chromosome 13q14, a Region Commonly Deleted in B-Cell Chronic Lymphocytic Leukemia
Cancer Res.,
April 1, 2001;
61(7):
2870 - 2877.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Migliazza, F. Bosch, H. Komatsu, E. Cayanis, S. Martinotti, E. Toniato, E. Guccione, X. Qu, M. Chien, V. V. V. Murty, et al.
Nucleotide sequence, transcription map, and mutation analysis of the 13q14 chromosomal region deleted in B-cell chronic lymphocytic leukemia
Blood,
April 1, 2001;
97(7):
2098 - 2104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Starostik, A. Greiner, A. Schultz, A. Zettl, K. Peters, A. Rosenwald, M. Kolve, and H. K. Muller-Hermelink
Genetic aberrations common in gastric high-grade large B-cell lymphoma
Blood,
February 15, 2000;
95(4):
1180 - 1187.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Cuneo, R. Bigoni, G. M. Rigolin, M. G. Roberti, A. Bardi, N. Piva, R. Milani, F. Bullrich, M. L. Veronese, C. Croce, et al.
Cytogenetic Profile of Lymphoma of Follicle Mantle Lineage: Correlation With Clinicobiologic Features
Blood,
February 15, 1999;
93(4):
1372 - 1380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Caligaris-Cappio and T. J. Hamblin
B-Cell Chronic Lymphocytic Leukemia: A Bird of a Different Feather
J. Clin. Oncol.,
January 1, 1999;
17(1):
399 - 399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
