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Establishing a KSHV+ Cell Line (BCP-1) From Peripheral
Blood and Characterizing Its Growth in Nod/SCID Mice
Chris Boshoff,
Shou-Jiang Gao,
Lyn E. Healy,
Steve Matthews,
Alero
J. Thomas,
Loinel Coignet,
Roger A. Warnke,
James A. Strauchen,
Estella Matutes,
Onsi W. Kamel,
Patrick S. Moore,
Robin A. Weiss, and
Yuan Chang
From the Chester Beatty Laboratories, Institute of Cancer Research,
London, UK; the Department of Pathology and School of Public Health,
Columbia University, New York, NY; the Clinical Sciences Department,
London School of Hygiene and Tropical Medicine, University of London,
London, UK; the Department of Pathology, Stanford University School of
Medicine, Stanford, CA; and the Department of Pathology, Mount Sinai
School of Medicine, New York, NY.
Kaposi's sarcoma-associated herpesvirus (KSHV or HHV8) sequences
are present in primary effusion lymphomas (PEL). KSHV+
cell lines have been established from such lymphomas. Here we report
the first description of the establishment of a KSHV+,
EBV cell line (BCP-1) from the peripheral blood of a
patient with PEL. Using this cell line and a KSHV+,
EBV+ PEL cell line (HBL-6) previously established from
ascitic fluid, we investigated whether in nonobese diabetic/severe
combined immunodeficiency disease (Nod/SCID) mice tumors representing
PEL can be established. When injected intravenously (IV) into Nod/SCID
mice, BCP-1 and HBL-6 infiltrated organs, with only occasional
macroscopic tumor formation. Intraperitoneal injections (ip) led to the
development of ascites and diffuse infiltration of organs, without
obviously solid lymphoma formation, resembling the diffuse nature of
human PEL. To investigate a possible mechanism for the peculiar
phenotype of PEL, we examine the presence of adhesion molecules and
homing markers on PEL cells before and after growing in mice. Both
BCP-1 and HBL-6 cells lack expression of important cytoadhesion
molecules including CD11a and CD18 (LFA1  and  chains), CD29,
CD31, CD44, CD54 (ICAM-1), and CD62L and E (L and E selectins).
Blood, Vol. 91 No. 5 (March 1), 1998:
pp. 1671-1679
© 1998 by The American Society of Hematology.
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J. Virol.,
February 15, 2007;
81(4):
1912 - 1922.
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M. M. Brinkmann, M. Pietrek, O. Dittrich-Breiholz, M. Kracht, and T. F. Schulz
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J. Virol.,
January 1, 2007;
81(1):
42 - 58.
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|
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S. A. Keller, D. Hernandez-Hopkins, J. Vider, V. Ponomarev, E. Hyjek, E. J. Schattner, and E. Cesarman
NF-{kappa}B is essential for the progression of KSHV- and EBV-infected lymphomas in vivo
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107(8):
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[Abstract]
[Full Text]
[PDF]
|
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|
|
|
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A. Cohen, C. Brodie, and R. Sarid
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J. Gen. Virol.,
April 1, 2006;
87(Pt 4):
795 - 802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Chen and M. Lagunoff
Establishment and Maintenance of Kaposi's Sarcoma-Associated Herpesvirus Latency in B Cells
J. Virol.,
November 15, 2005;
79(22):
14383 - 14391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Cuomo, A. Knebel, G. Platt, N. Morrice, P. Cohen, and S. Mittnacht
Regulation of Microfilament Organization by Kaposi Sarcoma-associated Herpes Virus-cyclin{middle dot}CDK6 Phosphorylation of Caldesmon
J. Biol. Chem.,
October 28, 2005;
280(43):
35844 - 35858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Gasperini, M. Barbierato, C. Martinelli, P. Rigotti, F. Marchini, G. Masserizzi, F. Leoncini, L. Chieco-Bianchi, T. F. Schulz, and M. L. Calabro
Use of a BJAB-Derived Cell Line for Isolation of Human Herpesvirus 8
J. Clin. Microbiol.,
June 1, 2005;
43(6):
2866 - 2875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Godfrey, J. Anderson, A. Papanastasiou, Y. Takeuchi, and C. Boshoff
Inhibiting primary effusion lymphoma by lentiviral vectors encoding short hairpin RNA
Blood,
March 15, 2005;
105(6):
2510 - 2518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Deng, Y.-J. Zhang, X.-P. Wang, and S.-J. Gao
Lytic Replication-Defective Kaposi's Sarcoma-Associated Herpesvirus: Potential Role in Infection and Malignant Transformation
J. Virol.,
October 15, 2004;
78(20):
11108 - 11120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Deutsch, A. Cohen, G. Kazimirsky, S. Dovrat, H. Rubinfeld, C. Brodie, and R. Sarid
Role of Protein Kinase C {delta} in Reactivation of Kaposi's Sarcoma-Associated Herpesvirus
J. Virol.,
September 15, 2004;
78(18):
10187 - 10192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Staudt, Y. Kanan, J. H. Jeong, J. F. Papin, R. Hines-Boykin, and D. P. Dittmer
The Tumor Microenvironment Controls Primary Effusion Lymphoma Growth in Vivo
Cancer Res.,
July 15, 2004;
64(14):
4790 - 4799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Canham and S. J. Talbot
A naturally occurring C-terminal truncated isoform of the latent nuclear antigen of Kaposi's sarcoma-associated herpesvirus does not associate with viral episomal DNA
J. Gen. Virol.,
June 1, 2004;
85(6):
1363 - 1369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Bieleski, C. Hindley, and S. J. Talbot
A polypyrimidine tract facilitates the expression of Kaposi's sarcoma-associated herpesvirus vFLIP through an internal ribosome entry site
J. Gen. Virol.,
March 1, 2004;
85(3):
615 - 620.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Trivedi, K. Takazawa, C. Zompetta, L. Cuomo, E. Anastasiadou, A. Carbone, S. Uccini, F. Belardelli, K. Takada, L. Frati, et al.
Infection of HHV-8+ primary effusion lymphoma cells with a recombinant Epstein-Barr virus leads to restricted EBV latency, altered phenotype, and increased tumorigenicity without affecting TCL1 expression
Blood,
January 1, 2004;
103(1):
313 - 316.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. G. Jenner, K. Maillard, N. Cattini, R. A. Weiss, C. Boshoff, R. Wooster, and P. Kellam
Kaposi's sarcoma-associated herpesvirus-infected primary effusion lymphoma has a plasma cell gene expression profile
PNAS,
September 2, 2003;
100(18):
10399 - 10404.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Brinkmann, M. Glenn, L. Rainbow, A. Kieser, C. Henke-Gendo, and T. F. Schulz
Activation of Mitogen-Activated Protein Kinase and NF-{kappa}B Pathways by a Kaposi's Sarcoma-Associated Herpesvirus K15 Membrane Protein
J. Virol.,
September 1, 2003;
77(17):
9346 - 9358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Dourmishev, A. L. Dourmishev, D. Palmeri, R. A. Schwartz, and D. M. Lukac
Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis
Microbiol. Mol. Biol. Rev.,
June 1, 2003;
67(2):
175 - 212.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Rosbottom, R. G. Dalziel, H. W. Reid, and J. P. Stewart
Ovine herpesvirus 2 lytic cycle replication and capsid production
J. Gen. Virol.,
December 1, 2002;
83(12):
2999 - 3002.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Li, T. Komatsu, B. J. Dezube, and K. M. Kaye
The Kaposi's Sarcoma-Associated Herpesvirus K12 Transcript from a Primary Effusion Lymphoma Contains Complex Repeat Elements, Is Spliced, and Initiates from a Novel Promoter
J. Virol.,
October 25, 2002;
76(23):
11880 - 11888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Cone, R. Nazemi, M. O. Cone, H.J. V. Giesen, R. Kaiser, H. Koller, K. Wetzel, G. Arendt, A. Moulignier, and D. MacGowan
Reversible ALS-like disorder in HIV infection An ALS-like syndrome with new HIV infection and complete response to antiretroviral therapy
Neurology,
August 13, 2002;
59(3):
474 - 475.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. Moses, M. A. Jarvis, C. Raggo, Y. C. Bell, R. Ruhl, B. G. M. Luukkonen, D. J. Griffith, C. L. Wait, B. J. Druker, M. C. Heinrich, et al.
Kaposi's Sarcoma-Associated Herpesvirus-Induced Upregulation of the c-kit Proto-Oncogene, as Identified by Gene Expression Profiling, Is Essential for the Transformation of Endothelial Cells
J. Virol.,
July 17, 2002;
76(16):
8383 - 8399.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-J. Chiou, L. J. Poole, P. S. Kim, D. M. Ciufo, J. S. Cannon, C. M. ap Rhys, D. J. Alcendor, J.-C. Zong, R. F. Ambinder, and G. S. Hayward
Patterns of Gene Expression and a Transactivation Function Exhibited by the vGCR (ORF74) Chemokine Receptor Protein of Kaposi's Sarcoma-Associated Herpesvirus
J. Virol.,
March 7, 2002;
76(7):
3421 - 3439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. N. Kakoola, J. Sheldon, N. Byabazaire, R. J. Bowden, E. Katongole-Mbidde, T. F. Schulz, and A. J. Davison
Recombination in human herpesvirus-8 strains from Uganda and evolution of the K15 gene
J. Gen. Virol.,
October 1, 2001;
82(10):
2393 - 2404.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Perez and S. Rudoy
Anti-CD20 Monoclonal Antibody Treatment of Human Herpesvirus 8-Associated, Body Cavity-Based Lymphoma with an Unusual Phenotype in a Human Immunodeficiency Virus-Negative Patient
Clin. Vaccine Immunol.,
September 1, 2001;
8(5):
993 - 996.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Ciufo, J. S. Cannon, L. J. Poole, F. Y. Wu, P. Murray, R. F. Ambinder, and G. S. Hayward
Spindle Cell Conversion by Kaposi's Sarcoma-Associated Herpesvirus: Formation of Colonies and Plaques with Mixed Lytic and Latent Gene Expression in Infected Primary Dermal Microvascular Endothelial Cell Cultures
J. Virol.,
June 15, 2001;
75(12):
5614 - 5626.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Low, M. Harries, H. Ye, M.-Q. Du, C. Boshoff, and M. Collins
Internal Ribosome Entry Site Regulates Translation of Kaposi's Sarcoma-Associated Herpesvirus FLICE Inhibitory Protein
J. Virol.,
March 15, 2001;
75(6):
2938 - 2945.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. Bieleski and S. J. Talbot
Kaposi's Sarcoma-Associated Herpesvirus vCyclin Open Reading Frame Contains an Internal Ribosome Entry Site
J. Virol.,
February 15, 2001;
75(4):
1864 - 1869.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Davidovici, I. Karakis, D. Bourboulia, S. Ariad, J.-C. Zong, D. Benharroch, N. Dupin, R. Weiss, G. Hayward, B. Sarov, et al.
Seroepidemiology and Molecular Epidemiology of Kaposi's Sarcoma-Associated Herpesvirus Among Jewish Population Groups in Israel
J Natl Cancer Inst,
February 7, 2001;
93(3):
194 - 202.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Deng, A. Young, and R. Sun
Auto-activation of the rta gene of human herpesvirus-8/Kaposi's sarcoma-associated herpesvirus
J. Gen. Virol.,
December 1, 2000;
81(12):
3043 - 3048.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. S. Cannon, D. Ciufo, A. L. Hawkins, C. A. Griffin, M. J. Borowitz, G. S. Hayward, and R. F. Ambinder
A New Primary Effusion Lymphoma-Derived Cell Line Yields a Highly Infectious Kaposi's Sarcoma Herpesvirus-Containing Supernatant
J. Virol.,
November 1, 2000;
74(21):
10187 - 10193.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Krithivas, D. B. Young, G. Liao, D. Greene, and S. D. Hayward
Human Herpesvirus 8 LANA Interacts with Proteins of the mSin3 Corepressor Complex and Negatively Regulates Epstein-Barr Virus Gene Expression in Dually Infected PEL Cells
J. Virol.,
October 15, 2000;
74(20):
9637 - 9645.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Maeda, T. Niimi, S. Sato, H. Kawaguchi, Y. Sugiura, S. Mori, and R. Ueda
Human Herpesvirus 8 Is Not Associated With Sarcoidosis in Japanese Patients
Chest,
October 1, 2000;
118(4):
923 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-S. Lee, X. Alvarez, S. Ishido, A. A. Lackner, and J. U. Jung
Inhibition of Intracellular Transport of B Cell Antigen Receptor Complexes by Kaposi's Sarcoma-associated Herpesvirus K1
J. Exp. Med.,
June 26, 2000;
192(1):
11 - 22.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Pica, A. Volpi, A. Serafino, M. Fraschetti, O. Franzese, and E. Garaci
Autocrine nerve growth factor is essential for cell survival and viral maturation in HHV-8-infected primary effusion lymphoma cells
Blood,
May 1, 2000;
95(9):
2905 - 2912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Carbone, A. Gloghini, D. Bontempo, P. Monini, U. Tirelli, R. Volpe, P. J. Browning, and G. Gaidano
Proliferation in HHV-8-Positive Primary Effusion Lymphomas Is Associated with Expression of HHV-8 Cyclin but Independent of p27kip1
Am. J. Pathol.,
April 1, 2000;
156(4):
1209 - 1215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Parravicini, B. Chandran, M. Corbellino, E. Berti, M. Paulli, P. S. Moore, and Y. Chang
Differential Viral Protein Expression in Kaposi’s Sarcoma-Associated Herpesvirus-Infected Diseases : Kaposi’s Sarcoma, Primary Effusion Lymphoma, and Multicentric Castleman’s Disease
Am. J. Pathol.,
March 1, 2000;
156(3):
743 - 749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Dupin, T. L. Diss, P. Kellam, M. Tulliez, M.-Q. Du, D. Sicard, R. A. Weiss, P. G. Isaacson, and C. Boshoff
HHV-8 is associated with a plasmablastic variant of Castleman disease that is linked to HHV-8-positive plasmablastic lymphoma
Blood,
February 15, 2000;
95(4):
1406 - 1412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Aoki, G. Tosato, Y. Nambu, A. Iwamoto, and R. Yarchoan
Detection of vascular endothelial growth factor in AIDS-related primary effusion lymphomas
Blood,
February 1, 2000;
95(3):
1109 - 1110.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Aoki and G. Tosato
Role of Vascular Endothelial Growth Factor/Vascular Permeability Factor in the Pathogenesis of Kaposi's Sarcoma-Associated Herpesvirus-Infected Primary Effusion Lymphomas
Blood,
December 15, 1999;
94(12):
4247 - 4254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Bello, A. J. Davison, M. A. Glenn, A. Whitehouse, N. Rethmeier, T. F. Schulz, and J. Barklie Clements
The human herpesvirus-8 ORF 57 gene and its properties
J. Gen. Virol.,
December 1, 1999;
80(12):
3207 - 3215.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. W. Wong, E. P. Bergquam, R. M. Swanson, F. W. Lee, S. M. Shiigi, N. A. Avery, J. W. Fanton, and M. K. Axthelm
Induction of B Cell Hyperplasia in Simian Immunodeficiency Virus–infected Rhesus Macaques with the Simian Homologue of Kaposi's Sarcoma–associated Herpesvirus
J. Exp. Med.,
September 20, 1999;
190(6):
827 - 840.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Osman, T. Kubo, J. Gill, F. Neipel, M. Becker, G. Smith, R. Weiss, B. Gazzard, C. Boshoff, and F. Gotch
Identification of Human Herpesvirus 8-Specific Cytotoxic T-Cell Responses
J. Virol.,
July 1, 1999;
73(7):
6136 - 6140.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Sitas, H. Carrara, V. Beral, R. Newton, G. Reeves, D. Bull, U. Jentsch, R. Pacella-Norman, D. Bourboulia, D. Whitby, et al.
Antibodies against Human Herpesvirus 8 in Black South African Patients with Cancer
N. Engl. J. Med.,
June 17, 1999;
340(24):
1863 - 1871.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Kellam, D. Bourboulia, N. Dupin, C. Shotton, C. Fisher, S. Talbot, C. Boshoff, and R. A. Weiss
Characterization of Monoclonal Antibodies Raised against the Latent Nuclear Antigen of Human Herpesvirus 8
J. Virol.,
June 1, 1999;
73(6):
5149 - 5155.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J.-C. Zong, D. M. Ciufo, D. J. Alcendor, X. Wan, J. Nicholas, P. J. Browning, P. L. Rady, S. K. Tyring, J. M. Orenstein, C. S. Rabkin, et al.
High-Level Variability in the ORF-K1 Membrane Protein Gene at the Left End of the Kaposi's Sarcoma-Associated Herpesvirus Genome Defines Four Major Virus Subtypes and Multiple Variants or Clades in Different Human Populations
J. Virol.,
May 1, 1999;
73(5):
4156 - 4170.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Dupin, C. Fisher, P. Kellam, S. Ariad, M. Tulliez, N. Franck, E. van Marck, D. Salmon, I. Gorin, J.-P. Escande, et al.
Distribution of human herpesvirus-8 latently infected cells in Kaposi's sarcoma, multicentric Castleman's disease, and primary effusion lymphoma
PNAS,
April 13, 1999;
96(8):
4546 - 4551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Jones, M. E. Ballestas, K. M. Kaye, J. M. Gulizia, G. L. Winters, J. Fletcher, D. T. Scadden, and J. C. Aster
Primary-Effusion Lymphoma and Kaposi's Sarcoma in a Cardiac-Transplant Recipient
N. Engl. J. Med.,
August 13, 1998;
339(7):
444 - 449.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lebbe
Human Herpesvirus 8 as the Infectious Cause of Kaposi Sarcoma: Evidence and Involvement of Cofactors
Arch Dermatol,
June 1, 1998;
134(6):
736 - 738.
[Full Text]
[PDF]
|
|
|
|
|
