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The Fc Receptor-Mediated Respiratory Burst of Rolling
Neutrophils to Cytokine-Activated, Immune Complex-Bearing
Endothelial Cells Depends on L-Selectin But Not on E-Selectin
Dieter Fröhlich,
Olivier Spertini, and
René Moser
From the Institute of Toxicology, Federal Institute of Technology,
Schwerzenbach; the Division and Central Laboratory of Hematology CHUV,
Lausanne, Switzerland; and the Department of Anesthesiology, University
of Regensburg, Regensburg, Germany.
Intracellular H2O2 generation, as a measure
of the respiratory burst, was determined after stimulation of
neutrophils by immune complex (IC)-bearing human umbilical vein
endothelial cells. Under static conditions, neutrophils basically
responded to the immune deposits on resting endothelial cells. The
rotating shear forces of  0.7 dynes/cm2, corresponding to
the physiological flow in postcapillary venules, completely abolished
this basal H2O2 generation. After activation of
the IC-bearing endothelial layers with interleukin-1 (IL-1) or tumor
necrosis factor (TNF), or both, for 4 hours, rolling adhesion of the
neutrophils was induced, accompanied by considerable H2O2 production. The neutrophil respiratory
burst was prominently inhibited by anti-Fc RIII MoAb 3G8 (72.4%),
and partially by MoAb 2E1 against FcRII (38.5%). Both MoAbs
together inhibited the Fc-mediated H2O2
generation by 93.4%. The respiratory burst and rolling adhesion were
markedly blocked by MoAb LAM1-3 against L-selectin (91.3%),
whereas the nonfunctional anti-L-selectin MoAb LAM1-14 was
ineffective. F(ab)2 fragments of MoAb 7A9 against E-selectin inhibited neutrophil rolling by 98.6%, but not
the respiratory burst. Moreover, rolling adhesion of neutrophils and the related oxidative burst were CD11b/CD18- independent. In summary, L-selectin has a unique auxiliary function in triggering the
FcR-mediated respiratory burst of rolling neutrophils to IC-bearing
endothelial cells, thereby substituting CD11b/CD18 under conditions of
flow.
Blood, Vol. 91 No. 7 (April 1), 1998:
pp. 2558-2564
© 1998 by The American Society of Hematology.
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