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Decay-Accelerating Factor (CD55) and Membrane Inhibitor of Reactive
Lysis (CD59) Are Released Within Exosomes During In Vitro
Maturation of Reticulocytes
Herisoa Rabesandratana,
Jean-Pierre Toutant,
Hubert Reggio, and
Michel Vidal
From the Hôpital St Eloi, Laboratoire Central
d'hématologie; INRA, Physiologie Animale; and UMR 5539, Université Montpellier II, Montpellier, France.
Exosomes are membrane vesicles released by reticulocytes during
their maturation into erythrocytes. They have a clearing function because of their enrichment with some proteins known to decrease or
disappear from the cell surface during maturation, eg,
acetylcholinesterase (AChE) and transferrin receptor (TfR),
respectively. To better understand the molecular events leading to
protein sorting in exosomes, we analyzed the expression of
glycosylphosphatidylinositol (GPI)-anchored proteins on the exosome
surface through a technique involving bead coupling and flow cytometry
immunodetection. The presence of AChE, decay-accelerating factor (DAF),
membrane inhibitor of reactive lysis (MIRL), and lymphocyte
function-associated antigen 3 (LFA-3) on the surface of exosomes
obtained from normal and paroxysmal nocturnal hemoglobinuria (PNH)
reticulocytes, suggests that (1) the GPI anchor is efficiently sorted
during exosome formation, (2) exosome release could account for the
observed discrepancy in GPI-protein expression between reticulocytes
and erythrocytes from PNH patients, and (3) exosomes could have another
physiologic function related to controlling membrane attack complex
formation.
Blood, Vol. 91 No. 7 (April 1), 1998:
pp. 2573-2580
© 1998 by The American Society of Hematology.
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