|
|
 Previous Article | Table of Contents | Next Article 
Single-Cell Analysis of the t(14;18)(q32;q21) Chromosomal
Translocation in Hodgkin's Disease Demonstrates the Absence of
This Translocation in Neoplastic Hodgkin and Reed-Sternberg Cells
Sylvia Gravel,
Georges Delsol, and
Talal Al Saati
From the Department of Pathology and CIGH/CNRS, CHU-Purpan, Toulouse,
France.
Using the polymerase chain reaction (PCR) technique and total DNA
extracts of Hodgkin's disease (HD)-involved lymph nodes, the
t(14;18)(q32;q21) translocation was detected in 37 of 115 (32.2%)
cases studied. No correlation was found between the presence of this
translocation and bcl-2 protein expression in Hodgkin and
Reed-Sternberg (HRS) cells detected by immunohistochemistry in 58 of 96 (60.4%) cases. To identify the cells carrying the t(14;18)
translocation, single-cell DNA from HRS cells isolated by
micromanipulation from frozen tissue sections of lymph nodes was
investigated by PCR amplification. Eleven cases showing a positive band
of the same size in at least two of five PCR experiments performed on
the same total DNA extract were selected for single-cell PCR. We
postulated that this repeated successful amplification could be
indicative of the presence of the t(14;18) translocation in the
neoplastic HRS cells. Single cells from frozen tumor sections of the
t(14;18)-positive OCI LY8 cell line grafted into nude mice served as a
positive control. The bcl-2/JH rearrangement, involved in
this translocation, could be amplified from single-cell DNA of the
latter tumor, whereas, in all of the HD cases, HRS cells were found to
be negative. We conclude that the t(14;18) translocation is not
localized in HRS cells, but in nonmalignant B bystander lymphocytes,
admixed with these neoplastic cells.
Blood, Vol. 91 No. 8 (April 15), 1998:
pp. 2866-2874
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. I. Martin-Subero, W. Klapper, A. Sotnikova, E. Callet-Bauchu, L. Harder, C. Bastard, R. Schmitz, S. Grohmann, J. Hoppner, J. Riemke, et al.
Chromosomal Breakpoints Affecting Immunoglobulin Loci Are Recurrent in Hodgkin and Reed-Sternberg Cells of Classical Hodgkin Lymphoma
Cancer Res.,
November 1, 2006;
66(21):
10332 - 10338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Re, R. Kuppers, and V. Diehl
Molecular Pathogenesis of Hodgkin's Lymphoma
J. Clin. Oncol.,
September 10, 2005;
23(26):
6379 - 6386.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Re, P. Starostik, N. Massoudi, A. Staratschek-Jox, V. Dries, R. K. Thomas, V. Diehl, and J. Wolf
Allelic Losses on Chromosome 6q25 in Hodgkin and Reed Sternberg Cells
Cancer Res.,
May 15, 2003;
63(10):
2606 - 2609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Maggio, A. van den Berg, D. de Jong, A. Diepstra, and S. Poppema
Low Frequency of FAS Mutations in Reed-Sternberg Cells of Hodgkin's Lymphoma
Am. J. Pathol.,
January 1, 2003;
162(1):
29 - 35.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Z. Rassidakis, L. J. Medeiros, T. P. Vassilakopoulos, S. Viviani, V. Bonfante, G. Nadali, M. Herling, M. K. Angelopoulou, R. Giardini, M. Chilosi, et al.
BCL-2 expression in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease predicts a poorer prognosis in patients treated with ABVD or equivalent regimens
Blood,
December 1, 2002;
100(12):
3935 - 3941.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Thomas, A. Kallenborn, C. Wickenhauser, J. L. Schultze, A. Draube, M. Vockerodt, D. Re, V. Diehl, and J. Wolf
Constitutive Expression of c-FLIP in Hodgkin and Reed-Sternberg Cells
Am. J. Pathol.,
April 1, 2002;
160(4):
1521 - 1528.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Faumont, T. Al Saati, P. Brousset, C. Offer, G. Delsol, and F. Meggetto
Demonstration by single-cell PCR that Reed-Sternberg cells and bystander B lymphocytes are infected by different Epstein-Barr virus strains in Hodgkin's disease
J. Gen. Virol.,
May 1, 2001;
82(5):
1169 - 1174.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Franke, I. Wlodarska, B. Maes, P. Vandenberghe, J. Delabie, A. Hagemeijer, and C. De Wolf-Peeters
Lymphocyte predominance Hodgkin disease is characterized by recurrent genomic imbalances
Blood,
March 15, 2001;
97(6):
1845 - 1853.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Jungnickel, A. Staratschek-Jox, A. Brauninger, T. Spieker, J. Wolf, V. Diehl, M.-L. Hansmann, K. Rajewsky, and R. Kuppers
Clonal Deleterious Mutations in the I{kappa}b{alpha} Gene in the Malignant Cells in Hodgkin's Lymphoma
J. Exp. Med.,
January 17, 2000;
191(2):
395 - 402.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
