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Expression of the T-Cell-Specific Tyrosine Kinase Lck in Normal B-1
Cells and in Chronic Lymphocytic Leukemia B Cells
M. Bernardetta Majolini,
Mario M. D'Elios, Piero Galieni,
Marianna Boncristiano,
Francesco Lauria,
Gianfranco Del Prete,
John L. Telford, and
Cosima T. Baldari
From the Department of Evolutionary Biology, University of Siena,
Siena, Italy; Internal Medicine and Immunoallergology, University of
Florence, Florence, Italy; the Division of Hematology, Ospedale A. Sclavo, Siena, Italy; and Chiron Research Center, Siena, Italy.
Src family kinases play a key role in mitogenesis. The exquisitely
tissue-specific distribution of different Src family members suggests
that a fine tuning of their expression might be a key prerequisite for
cell homeostasis. We tested B cells from patients affected by B-cell
chronic lymphocytic leukemia (B-CLL) for expression of Src family
kinases. The T-cell-specific tyrosine kinase Lck was found to be
expressed at significant levels in CLL B-cells. This finding could be
accounted for either by ectopic expression of Lck in B-CLL or by
specific expression of this kinase in normal B-1 cells, which are
believed to be the normal counterpart of CLL B cells. To answer this
question B cells from different sources, characterized by a different
size of the B-1 subpopulation, were tested for Lck expression. The
results show that Lck expression is a feature of CD5+,
B-1 cells, suggesting a potential role for Lck in the self-renewal capacity of this B-cell subpopulation and supporting the notion that
B-1 cells are the subset undergoing oncogenic transformation in B-CLL.
Furthermore, we show that the CD5 , B-2 subpopulation,
while normally lacking Lck expression, acquires the capacity to express
Lck ectopically upon transformation by EBV.
Blood, Vol. 91 No. 9 (May 1), 1998:
pp. 3390-3396
© 1998 by The American Society of Hematology.
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