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Intracellular Cytokine Profile of Cord and Adult Blood Lymphocytes
I.M.H. Chalmers,
G. Janossy,
M. Contreras, and
C. Navarrete
From the Department of Histocompatibility and Immunogenetics, North
London Centre (NBS), London, UK; and the Department of Immunology,
Royal Free Hospital, Pond Street, London, UK.
Umbilical cord blood (CB) transplantation is thought to be
associated with a reduced risk of severe graft-versus-host-disease (GVHD) compared with bone marrow transplantation (BMT). The cytokine cascade is known to be important in the pathogenesis of GVHD; however,
previous studies investigating the cytokine secretion pattern of CB
cells have been contradictory because of variations in experimental
techniques. In this study, the cytokine profile of cord and adult blood
lymphocytes and lymphocyte subsets has been assessed at the single-cell
level by flow cytometry, using CD4/CD8 and CD45RA/CD45RO markers. Cord
and adult blood mononuclear cells were stimulated with phorbol
12-myristate 13-acetate (PMA) and ionomycin in the presence of
monensin. After 4 to 24 hours of incubation, interleukin-2 (IL-2),
IL-4, interferon- (IFN-), and tumor necrosis factor- (TNF-)
production was measured by three-color flow cytometry. The results show
that cord blood lymphocytes (CBL) produce less IL-2, IL-4, IFN-, and
TNF- than adult peripheral blood lymphocytes (ABL). Further subset
analysis showed that in CBL the majority of cytokine producing cells
were CD4+CD45RA+, whereas in ABL the
cytokine-producing cells were both
CD4+CD45RO+ and
CD8+CD45RO+. These results suggest that the
reduced incidence of GVHD in CB transplantation may partly due to the
altered cytokine profile seen in CBL.
Blood, Vol. 92 No. 1 (July 1), 1998:
pp. 11-18
© 1998 by The American Society of Hematology.
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