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Induction of Synaptosomal-Associated Protein-23 kD (SNAP-23) by Various Cytokines

Yoshihiro Morikawa, Hitoshi Nishida, Kazuhide Misawa, Tetsuya Nosaka, Atsushi Miyajima, Emiko Senba, and Toshio Kitamura

From the Department of Anatomy and Neurobiology, Wakayama Medical School, Wakayama; the Department of Hemopoietic Factors, the Institute of Medical Science, University of Tokyo; and the Laboratory of Cellular Biosynthesis, the Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo, Japan.

Cytokines manifest their function through regulation of gene expression. We searched for immediate-early cytokine responsive genes by the mRNA differential display technique using interleukin-3 (IL-3)-dependent OTT-1 cells, and have isolated a novel cDNA which encodes 210 amino acids and shows 87% amino acid identity to human SNAP-23 (synaptosomal-associated protein of 23 kD). The message for this protein (mouse SNAP-23) was induced in OTT-1 cells by IL-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-5. The experiment using C-terminal deletion mutants of the common beta  subunit (beta c) of IL-3/GM-CSF/IL-5 receptors showed that expression of SNAP-23 was associated with the Ras-Raf-MAPK pathway, but not with the JAK-STAT pathway. Moreover, SNAP-23 was induced in response to a wide variety of cytokines, including IL-2, IL-3, IL-5, IL-10, stem cell factor, G-CSF, GM-CSF, leukemia inhibitory factor, and erythropoietin. Constitutive expression of SNAP-23 was seen in various tissues, including heart, lung, kidney, liver, spleen, and small intestine. Possible involvement of SNAP-23 in cytokine signal transduction is discussed.

Blood, Vol. 92 No. 1 (July 1), 1998: pp. 129-135
© 1998 by The American Society of Hematology.


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