|
|
 Previous Article | Table of Contents | Next Article 
Adhesion, Transendothelial Migration, and Reverse Transmigration
of In Vitro Cultured Dendritic Cells
Giovanna D'Amico,
Giancarlo Bianchi,
Sergio Bernasconi,
Laura Bersani,
Lorenzo Piemonti,
Silvano Sozzani,
Alberto Mantovani, and
Paola Allavena
From the Department of Immunology and Cell Biology, "Mario
Negri" Institute, Milan; and the Section of General Pathology,
University of Brescia, Brescia, Italy.
Dendritic cells (DC) are migratory cells which exhibit complex
trafficking properties in vivo, involving interaction with vascular and
lymphatic endothelium and extracellular matrix (ECM). The underlying
mechanisms involved in these processes are still ill defined. In the
present study we have investigated the ability of DC to interact in
vitro with human vascular endothelial cells (EC) and ECM. DC were
differentiated from monocytes by in vitro exposure to
granulocyte-macrophage colony-stimulating factor and interleukin-13 for
7 days. In adhesion assays a considerable proportion of DC bound to
resting EC monolayers: (17% ± 4%, mean ± SE of eight
experiments). Adhesion to tumor necrosis factor (TNF)-activated EC was
increased to 29% ± 5% (n = 8). Binding to resting EC was strongly inhibited by anti-CD11a and CD11b, but not by CD11c monoclonal antibodies (MoAbs); on TNF-activated EC, anti-VLA-4 in concert with
anti-CD18 inhibited adhesion by more than 70%. Binding to a natural
ECM, derived from cultured EC, or to purified fibronectin was high:
52% ± 6% (n = 8) involved VLA-4 and VLA-5
integrins. In a transmigration assay, 10% ± 2% (n = 6) of input
cells were able to cross the EC monolayer. Unlike adhesion,
transendothelial migration was significantly reduced by anti-CD31 MoAb.
The amount of DC transmigrated through a monolayer of EC was increased
twofold to threefold by a defined set of C-C chemokines including
RANTES, MIP1 , MIP5, and, to a lesser extent, by MIP1
and MCP-3. Most importantly, in view of the trafficking pattern of
these cells, a significant proportion of DC (13% ± 4% of input
cells seeded) was able to migrate across the endothelial basement
membrane and, subsequently, across the endothelial barrier (reverse
transmigration). The adhesion molecules and chemoattractants
characterized herein are likely to underlie the complex trafficking of
DC in vivo.
Blood, Vol. 92 No. 1 (July 1), 1998:
pp. 207-214
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. THIEL, R. PRIES, S. JESKE, T. TRENKLE, and B. WOLLENBERG
Effect of Head and Neck Cancer Supernatant and CpG-Oligonucleotides on Migration and IFN-{alpha} Production of Plasmacytoid Dendritic Cells
Anticancer Res,
August 1, 2009;
29(8):
3019 - 3025.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. Gomez, R. Pilcher-Roberts, A. Alborzi, and S. L. Newman
Histoplasma capsulatum Cyclophilin A Mediates Attachment to Dendritic Cell VLA-5
J. Immunol.,
November 15, 2008;
181(10):
7106 - 7114.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mancino, T. Schioppa, P. Larghi, F. Pasqualini, M. Nebuloni, I-H. Chen, S. Sozzani, J. M. Austyn, A. Mantovani, and A. Sica
Divergent effects of hypoxia on dendritic cell functions
Blood,
November 1, 2008;
112(9):
3723 - 3734.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. van Vliet, L. C. Paessens, V. C. M. Broks-van den Berg, T. B. H. Geijtenbeek, and Y. van Kooyk
The C-Type Lectin Macrophage Galactose-Type Lectin Impedes Migration of Immature APCs
J. Immunol.,
September 1, 2008;
181(5):
3148 - 3155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Harwood, H. Gerber, F. Sobrino, A. Summerfield, and K. C. McCullough
Dendritic Cell Internalization of Foot-and-Mouth Disease Virus: Influence of Heparan Sulfate Binding on Virus Uptake and Induction of the Immune Response
J. Virol.,
July 1, 2008;
82(13):
6379 - 6394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Lehner, A. Stilper, P. Morhart, and W. Holter
Plasticity of dendritic cell function in response to prostaglandin E2 (PGE2) and interferon-{gamma} (IFN-{gamma})
J. Leukoc. Biol.,
April 1, 2008;
83(4):
883 - 893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Mendes-da-Cruz, S. Smaniotto, A. C. Keller, M. Dardenne, and W. Savino
Multivectorial Abnormal Cell Migration in the NOD Mouse Thymus
J. Immunol.,
April 1, 2008;
180(7):
4639 - 4647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. F. Bradfield, C. Scheiermann, S. Nourshargh, C. Ody, F. W. Luscinskas, G. E. Rainger, G. B. Nash, M. Miljkovic-Licina, M. Aurrand-Lions, and B. A. Imhof
JAM-C regulates unidirectional monocyte transendothelial migration in inflammation
Blood,
October 1, 2007;
110(7):
2545 - 2555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-L. Hahn, A.M. Best, and J.G. Tew
Rapid Tissue Factor Induction by Oral Streptococci and Monocyte-IL-1{beta}
Journal of Dental Research,
March 1, 2007;
86(3):
255 - 259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S Ranjit and L Dazhu
Potential role of dendritic cells for progression of atherosclerotic lesions.
Postgrad. Med. J.,
September 1, 2006;
82(971):
573 - 575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. G. van Helden, D. J. E. B. Krooshoop, K. C. M. Broers, R. A. P. Raymakers, C. G. Figdor, and F. N. van Leeuwen
A Critical Role for Prostaglandin E2 in Podosome Dissolution and Induction of High-Speed Migration during Dendritic Cell Maturation
J. Immunol.,
August 1, 2006;
177(3):
1567 - 1574.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Benhamron, H. Nechushtan, I. Verbovetski, A. Krispin, G. Abboud-Jarrous, E. Zcharia, E. Edovitsky, E. Nahari, T. Peretz, I. Vlodavsky, et al.
Translocation of Active Heparanase to Cell Surface Regulates Degradation of Extracellular Matrix Heparan Sulfate upon Transmigration of Mature Monocyte-Derived Dendritic Cells.
J. Immunol.,
June 1, 2006;
176(11):
6417 - 6424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sharma and D.-Z. Li
Role of Dendritic Cells in Atherosclerosis
Asian Cardiovasc Thorac Ann,
April 1, 2006;
14(2):
166 - 169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Chabot, P. Reverdiau, S. Iochmann, A. Rico, D. Senecal, C. Goupille, P.-Y. Sizaret, and L. Sensebe
CCL5-enhanced human immature dendritic cell migration through the basement membrane in vitro depends on matrix metalloproteinase-9
J. Leukoc. Biol.,
April 1, 2006;
79(4):
767 - 778.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Osterholzer, T. Ames, T. Polak, J. Sonstein, B. B. Moore, S. W. Chensue, G. B. Toews, and J. L. Curtis
CCR2 and CCR6, but Not Endothelial Selectins, Mediate the Accumulation of Immature Dendritic Cells within the Lungs of Mice in Response to Particulate Antigen
J. Immunol.,
July 15, 2005;
175(2):
874 - 883.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gough, M. Crittenden, U. Thanarajasingam, L. Sanchez-Perez, J. Thompson, D. Jevremovic, and R. Vile
Gene Therapy to Manipulate Effector T Cell Trafficking to Tumors for Immunotherapy
J. Immunol.,
May 1, 2005;
174(9):
5766 - 5773.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. D'Amico, M. Vulcano, C. Bugarin, G. Bianchi, G. Pirovano, M. Bonamino, V. Marin, P. Allavena, E. Biagi, and A. Biondi
CD40 activation of BCP-ALL cells generates IL-10-producing, IL-12-defective APCs that induce allogeneic T-cell anergy
Blood,
August 1, 2004;
104(3):
744 - 751.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. V. Gulino, D. Moratto, S. Sozzani, P. Cavadini, K. Otero, L. Tassone, L. Imberti, S. Pirovano, L. D. Notarangelo, R. Soresina, et al.
Altered leukocyte response to CXCL12 in patients with warts hypogammaglobulinemia, infections, myelokathexis (WHIM) syndrome
Blood,
July 15, 2004;
104(2):
444 - 452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Badolato
Leukocyte circulation: one-way or round-trip? Lessons from primary immunodeficiency patients
J. Leukoc. Biol.,
July 1, 2004;
76(1):
1 - 6.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Perrier, F. O. Martinez, M. Locati, G. Bianchi, M. Nebuloni, G. Vago, F. Bazzoni, S. Sozzani, P. Allavena, and A. Mantovani
Distinct Transcriptional Programs Activated by Interleukin-10 with or without Lipopolysaccharide in Dendritic Cells: Induction of the B Cell-Activating Chemokine, CXC Chemokine Ligand 13
J. Immunol.,
June 1, 2004;
172(11):
7031 - 7042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Kobayashi, S. Miura, H. Nagata, Y. Tsuzuki, R. Hokari, T. Ogino, C. Watanabe, T. Azuma, and H. Ishii
In situ demonstration of dendritic cell migration from rat intestine to mesenteric lymph nodes: relationships to maturation and role of chemokines
J. Leukoc. Biol.,
March 1, 2004;
75(3):
434 - 442.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chen, J. Guo, M. Yang, C. Han, M. Zhang, W. Chen, Q. Liu, J. Wang, and X. Cao
Cyclosporin A impairs dendritic cell migration by regulating chemokine receptor expression and inhibiting cyclooxygenase-2 expression
Blood,
January 15, 2004;
103(2):
413 - 421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Guan, G. Zu, Y. Xie, H. Tang, M. Johnson, X. Xu, C. Kevil, W.-C. Xiong, C. Elmets, Y. Rao, et al.
Neuronal Repellent Slit2 Inhibits Dendritic Cell Migration and the Development of Immune Responses
J. Immunol.,
December 15, 2003;
171(12):
6519 - 6526.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Chieppa, G. Bianchi, A. Doni, A. Del Prete, M. Sironi, G. Laskarin, P. Monti, L. Piemonti, A. Biondi, A. Mantovani, et al.
Cross-Linking of the Mannose Receptor on Monocyte-Derived Dendritic Cells Activates an Anti-Inflammatory Immunosuppressive Program
J. Immunol.,
November 1, 2003;
171(9):
4552 - 4560.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. de la Rosa, N. Longo, J. L. Rodriguez-Fernandez, A. Puig-Kroger, A. Pineda, A. L. Corbi, and P. Sanchez-Mateos
Migration of human blood dendritic cells across endothelial cell monolayers: adhesion molecules and chemokines involved in subset-specific transmigration
J. Leukoc. Biol.,
May 1, 2003;
73(5):
639 - 649.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. J. M. de Vries, D. J. E. B. Krooshoop, N. M. Scharenborg, W. J. Lesterhuis, J. H. S. Diepstra, G. N. P. van Muijen, S. P. Strijk, T. J. Ruers, O. C. Boerman, W. J. G. Oyen, et al.
Effective Migration of Antigen-pulsed Dendritic Cells to Lymph Nodes in Melanoma Patients Is Determined by Their Maturation State
Cancer Res.,
January 1, 2003;
63(1):
12 - 17.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rosenzwajg, F. Jourquin, L. Tailleux, and J. C. Gluckman
CD40 ligation and phagocytosis differently affect the differentiation of monocytes into dendritic cells
J. Leukoc. Biol.,
December 1, 2002;
72(6):
1180 - 1189.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Weis, C. L. Schlichting, E. G. Engleman, and J. P. Cooke
Endothelial Determinants of Dendritic Cell Adhesion and Migration: New Implications for Vascular Diseases
Arterioscler Thromb Vasc Biol,
November 1, 2002;
22(11):
1817 - 1823.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fiorini, W. Vermi, F. Facchetti, D. Moratto, G. Alessandri, L. Notarangelo, A. Caruso, P. Grigolato, A. G. Ugazio, L. D. Notarangelo, et al.
Defective migration of monocyte-derived dendritic cells in LAD-1 immunodeficiency
J. Leukoc. Biol.,
October 1, 2002;
72(4):
650 - 656.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Lokshin, P. Kalinski, R. R. Sassi, R. B. Mailliard, J. Muller-Berghaus, W. J. Storkus, X. Peng, A. M. Marrangoni, R. P. Edwards, and E. Gorelik
Differential regulation of maturation and apoptosis of human monocyte-derived dendritic cells mediated by MHC class II
Int. Immunol.,
September 1, 2002;
14(9):
1027 - 1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. Cutler, R. Jotwani, and B. Pulendran
Dendritic Cells: Immune Saviors or Achilles' Heel?
Infect. Immun.,
August 1, 2001;
69(8):
4703 - 4708.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Spottl, M. Hausmann, M. Kreutz, A. Peuker, D. Vogl, J. Scholmerich, W. Falk, R. Andreesen, T. Andus, H. Herfarth, et al.
Monocyte differentiation in intestine-like macrophage phenotype induced by epithelial cells
J. Leukoc. Biol.,
August 1, 2001;
70(2):
241 - 251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Yang, Q. Chen, S. Stoll, X. Chen, O. M. Z. Howard, and J. J. Oppenheim
Differential Regulation of Responsiveness to fMLP and C5a Upon Dendritic Cell Maturation: Correlation with Receptor Expression
J. Immunol.,
September 1, 2000;
165(5):
2694 - 2702.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ferrero, K. Vettoretto, A. Bondanza, A. Villa, M. Resnati, A. Poggi, and M. R. Zocchi
uPA/uPAR System Is Active in Immature Dendritic Cells Derived from CD14+CD34+ Precursors and Is Down-Regulated upon Maturation
J. Immunol.,
January 15, 2000;
164(2):
712 - 718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Yang, O. M. Z. Howard, Q. Chen, and J. J. Oppenheim
Cutting Edge: Immature Dendritic Cells Generated from Monocytes in the Presence of TGF-{beta}1 Express Functional C-C Chemokine Receptor 6
J. Immunol.,
August 15, 1999;
163(4):
1737 - 1741.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-A. Kellermann, S. Hudak, E. R. Oldham, Y.-J. Liu, and L. M. McEvoy
The CC Chemokine Receptor-7 Ligands 6Ckine and Macrophage Inflammatory Protein-3{beta} Are Potent Chemoattractants for In Vitro- and In Vivo-Derived Dendritic Cells
J. Immunol.,
April 1, 1999;
162(7):
3859 - 3864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
