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Blood, Vol. 92 No. 10 (November 15), 1998:
pp. 3616-3623
Two New Pseudopod Morphologies Displayed by the Human Hematopoietic
KG1a Progenitor Cell Line and by Primary Human CD34+
Cells
Karl Francis,
Ramprasad Ramakrishna,
William Holloway, and
Bernhard O. Palsson
From the Department of Bioengineering, University of California San
Diego, La Jolla, CA.
A primitive human hematopoietic myeloid progenitor cell line, KG1a,
characterized by high expression of the CD34 surface antigen has been
observed to extend long, thin pseudopodia. Once extended, these
pseudopods may take on one of two newly described morphologies, tenupodia or magnupodia. Tenupodia are very thin and form in linear segments. They adhere to the substrate, can bifurcate multiple times,
and often appear to connect the membranes of cells more than 300 µm
apart. Magnupodia are much thicker and have been observed to extend
more than 330 µm away from the cell. Magnupods are flexible and can
exhibit rapid dynamic motion, extending or retracting in a few seconds.
During retraction, the extended material often pools into a bulb
located on the pod. Both morphologies can adhere to substrates coated
with fibronectin, collagen IV, and laminin as well as plastic. The CD34
and CD44 antigens are also present on the surface of these podia.
Primary human CD34+ cells from fetal liver, umbilical
cord blood, adult bone marrow, and mobilized peripheral blood extend
these podia as well. The morphology that these pseudopods exhibit
suggest that they may play both sensory and mechanical roles during
cell migration and homing after bone marrow transplantation.
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