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Blood, Vol. 92 No. 11 (December 1), 1998:
pp. 4269-4278
Identification of New Nonrandom Translocations in Multiple
Myeloma With Multicolor Spectral Karyotyping
Jeffrey R. Sawyer,
Janet L. Lukacs,
Nikhil Munshi,
K. Raman Desikan,
Seema Singhal,
Jayesh Mehta,
David Siegel,
John Shaughnessy, and
Bart Barlogie
From the Departments of Pathology, Medicine, and the Myeloma and
Transplantation Research Center, Arkansas Cancer Research Center,
University of Arkansas for Medical Sciences, Little Rock; and
Cytogenetics Laboratory, Arkansas Children's Hospital, Little Rock,
AR.
Multicolor spectral karyotyping (SKY) was performed on bone marrow
samples from 50 patients with multiple myeloma (MM) in anticipation of
discovering new previously unidentified translocations. All samples
showed complex karyotypes with chromosome aberrations which, in most
cases, were not fully characterized by G-banding. Patients of special
interest were those who showed add(14)(q32), add(8)(q24) and those
whose G-banding karyotypes showed poor chromosome morphology. Three new
recurring chromosome translocations not previously reported in MM were
identified. Two of the translocations involve recurring aberrations at
band 14q32.3, the site of the IgH locus, with different exchange
partners. The most frequently recurring rearrangement was a subtle
translocation at 14q32.3 designated as a t(14;16)(q32;q22~23), which
was identified in six patients. A second and larger translocation at
14q32, identified in two patients, was designated as a
t(9;14)(p13;q32), previously associated with Waldenstrom's
macroglobulinemia and lymphoplasmacytoid lymphoma. A third
translocation, identified in two patients, involved a whole-arm
t(6;8)(p10;q10) translocation. The SKY technique was able to refine the
designations of over 156 aberrations not fully characterized by
G-banding in this study and resolved additional chromosome aberrations
in every patient studied except two. The t(14;16)(q32;q22~23)
identified by SKY in this study suggests this may be a frequent
translocation in MM associated with complex karyotypes and disease
progression. Therefore, the SKY technique provides a useful adjunct to
routine G-banding and fluorescence in situ hybridization studies in the
cytogenetic analysis of MM.
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