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Telomerase Activity in Hodgkin's Disease
Karl-Fredrik Norrback,
Gunilla Enblad,
Martin Erlanson,
Christer Sundström, and
Göran Roos
From the Departments of Pathology and Oncology, Umeå University,
Umeå, Sweden; and the Departments of Oncology and Pathology, Uppsala
University, Uppsala, Sweden.
Telomere maintenance executed by the action of telomerase seems to
be a prerequisite for immortalization. Telomerase is found in most cell
lines and malignant tumors. A telomerase-independent mechanism for
telomere maintenance in Hodgkin's disease has been proposed in the
absence of detectable telomerase activity. In this study, telomerase
activity was detected in 31 of 77 Hodgkin's disease samples and a
strong correlation between eosinophilia and absence of detectable
telomerase activity was found. Purified eosinophils and specifically
eosinophil-derived neurotoxin and eosinophilic cationic protein, both
ribonucleases, were found to degrade telomerase. Purified neutrophils
also exhibited weak telomerase degradative activity. Reanalysis of
previously telomerase-negative Hodgkin's disease samples with
eosinophilia using ribonuclease inhibitors resulted in the detection of
telomerase activity. Ribonuclease-containing cells in vivo thus have a
considerable impact on the detectability of telomerase. In Hodgkin's
disease samples without eosinophilia, 24 of 27 exhibited telomerase
activity at decreased levels compared with non-Hodgkin's lymphomas and
at increased levels compared with reactive nodes indicative of a
telomerase positive tumor component in Hodgkin's disease. Telomerase
positivity of the Hodgkin's and Reed-Sternberg cells in vivo was also
supported by high levels of telomerase expression in Hodgkin's disease
cell lines. Based on our data, Hodgkin's lymphomas are potential
targets for antitelomerase therapy.
Blood, Vol. 92 No. 2 (July 15), 1998:
pp. 567-573
© 1998 by the American Society of Hematology.
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