|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 92 No. 3 (August 1), 1998:
pp. 1031-1043
B-Cell Chronic Lymphocytic Leukemia Cells Express a Functional
Inducible Nitric Oxide Synthase Displaying Anti-Apoptotic Activity
Haixia Zhao,
Nathalie Dugas,
Claire Mathiot,
Alain Delmer,
Bernard Dugas,
François Sigaux, and
Jean-Pierre Kolb
From INSERM U365 and Service d'Hématologie, Institut Curie,
Paris; Laboratoire virus, neurone et immunité, UFR Kremlin
Bicêtre, Kremlin Bicêtre, Service d'Hématologie,
Hôpital de l'Hôtel Dieu, Paris; Fractales Biotech,
Hôpital Saint Joseph, Paris; and INSERM U462, Hôpital Saint
Louis, Paris.
The expression of different isoforms of nitric oxide synthase (NOS)
was investigated in B-cell chronic lymphocytic leukemia (B-CLL) to
delineate a possible role for nitric oxide (NO) in the control of
apoptosis of the tumoral cells. By reverse transcription-polymerase chain reaction (RT-PCR), all B-CLL cells were found to express spontaneously inducible NOS (iNOS) mRNA, whereas endothelial
constitutive NOS (ecNOS) mRNA was undetectable. The iNOS protein was
detected by immunofluorescence in the cytoplasm of permeabilized
leukemic cells and identified by Western blotting, using different
anti-iNOS antibodies, as a protein of 135 kD in B-CLL
cytoplasmic extracts. B-CLL cell lysates also displayed basal NOS
enzymatic activity, as measured by the conversion of
14C-labeled L-arginine into 14C-L-citrulline.
Ligation of CD23, expressed on the vast majority of B-CLL cells,
resulted in increased iNOS expression and activity. The NO released
exerted an anti-apoptotic effect on B-CLL cells that was counteracted
by NOS inhibitors and engagement of the APO-1/Fas pathway. Therefore,
the existence of a functional iNOS in B-CLL cells will provide further
insights into the mechanisms that control proliferation and apoptosis
in these tumor cells.
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Muerkoster, K. Wegehenkel, A. Arlt, M. Witt, B. Sipos, M.-L. Kruse, T. Sebens, G. Kloppel, H. Kalthoff, U. R. Folsch, et al.
Tumor Stroma Interactions Induce Chemoresistance in Pancreatic Ductal Carcinoma Cells Involving Increased Secretion and Paracrine Effects of Nitric Oxide and Interleukin-1{beta}
Cancer Res.,
February 15, 2004;
64(4):
1331 - 1337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kern, J.-F. Cornuel, C. Billard, R. Tang, D. Rouillard, V. Stenou, T. Defrance, F. Ajchenbaum-Cymbalista, P.-Y. Simonin, S. Feldblum, et al.
Involvement of BAFF and APRIL in the resistance to apoptosis of B-CLL through an autocrine pathway
Blood,
January 15, 2004;
103(2):
679 - 688.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D.-E. Hu, S. O. M. Dyke, A. M. Moore, L. L. Thomsen, and K. M. Brindle
Tumor Cell-Derived Nitric Oxide Is Involved in the Immune-Rejection of an Immunogenic Murine Lymphoma
Cancer Res.,
January 1, 2004;
64(1):
152 - 161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, A. Gonelli, C. Celeghini, P. Mirandola, L. Guidotti, G. Visani, S. Capitani, and G. Zauli
Activation of the nitric oxide synthase pathway represents a key component of tumor necrosis factor-related apoptosis-inducing ligand-mediated cytotoxicity on hematologic malignancies
Blood,
October 1, 2001;
98(7):
2220 - 2228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Feger, H. Ferry-Dumazet, M. M. Matsuda, J. Bordenave, M. Dupouy, A. K. Nussler, M. Arock, L. Devevey, J. Nafziger, J.-J. Guillosson, et al.
Role of Iron in Tumor Cell Protection from the Pro-Apoptotic Effect of Nitric Oxide
Cancer Res.,
July 1, 2001;
61(13):
5289 - 5294.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Mori, Y. Nunokawa, Y. Yamada, S. Ikeda, M. Tomonaga, and N. Yamamoto
Expression of Human Inducible Nitric Oxide Synthase Gene in T-Cell Lines Infected With Human T-Cell Leukemia Virus Type-I and Primary Adult T-Cell Leukemia Cells
Blood,
October 15, 1999;
94(8):
2862 - 2870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-M. Kim, C. A. Bombeck, and T. R. Billiar
Nitric Oxide as a Bifunctional Regulator of Apoptosis
Circ. Res.,
February 19, 1999;
84(3):
253 - 256.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Guereno, R. A. Diez;, B. Bellosillo, and J. Gil
Induction of Apoptosis by Salicylates in B-Cell Chronic Lymphocytic Leukemia
Blood,
February 1, 1999;
93(3):
1123 - 1124.
[Full Text]
[PDF]
|
|
|
|
|
