|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 92 No. 4 (August 15), 1998:
pp. 1225-1234
Bcl-3 Expression and Nuclear Translocation Are Induced by
Granulocyte-Macrophage Colony-Stimulating Factor and Erythropoietin in
Proliferating Human Erythroid Precursors
Min-Ying Zhang,
Edward W. Harhaj,
Laurie Bell,
Shao-Cong Sun, and
Barbara A. Miller
From the Departments of Pediatrics and Microbiology and Immunology,
The Pennsylvania State University College of Medicine, The Milton S. Hershey Medical Center, Hershey, PA.
Bcl-3 is a proto-oncogene involved in the chromosomal
translocation t(14;19) found in some patients with chronic lymphocytic leukemia. It shares structural similarities with and is a member of the
I B family of proteins. In this report, involvement of Bcl-3
in hematopoietic growth factor-stimulated erythroid proliferation and
differentiation was examined. In TF-1 cells, an erythroleukemia cell
line, granulocyte-macrophage colony-stimulating factor (GM-CSF) and
erythropoietin (Epo) greatly enhanced Bcl-3 expression at both
the protein and mRNA levels in association with stimulation of
proliferation. Bcl-3 protein was also highly expressed in early burst-forming unit-erythroid (BFU-E)-derived erythroid
precursors (day 7) and decreased during maturation (days 10 and 14),
suggesting that Bcl-3 is involved in normal erythroid proliferation. In
these hematopoietic cells, Bcl-3 was hyperphosphorylated. GM-CSF and Epo modulated the subcellular localization of Bcl-3. Upon stimulation of TF-1 cells with GM-CSF or Epo, the nuclear translocation of Bcl-3 was dramatically enhanced. Overexpression of Bcl-3 in TF-1 cells by transient transfection along with the NF-B factors p50 or
p52 resulted in significant induction of an human immunodeficiency virus-type 1 (HIV-1) B-TATA-luceriferase reporter plasmid,
demonstrating that Bcl-3 has a positive role in transactivation of
B-containing genes in erythroid cells. Stimulation with GM-CSF
enhanced c-myb mRNA expression in these cells. Bcl-3 in nuclear
extracts of TF-1 cells bound to a B enhancer in the c-myb
promoter together with NF-B2/p52 and this binding activity was
enhanced by GM-CSF stimulation. Furthermore, cotransfection of Bcl-3
with p52 or p50 in TF-1 cells resulted in significant activation of a
c-myb B-TATA-luceriferase reporter plasmid. These findings
suggest that Bcl-3 may participate in the transcriptional regulation of
certain B-containing genes involved in hematopoiesis, including
c-myb.
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Q. Tong, I. Hirschler-Laszkiewicz, W. Zhang, K. Conrad, D. W. Neagley, D. L. Barber, J. Y. Cheung, and B. A. Miller
TRPC3 Is the Erythropoietin-regulated Calcium Channel in Human Erythroid Cells
J. Biol. Chem.,
April 18, 2008;
283(16):
10385 - 10395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Rezzoug, Y. Huang, M. K. Tanner, M. Wysoczynski, C. L. Schanie, P. M. Chilton, M. Z. Ratajczak, I. J. Fugier-Vivier, and S. T. Ildstad
TNF-{alpha} Is Critical to Facilitate Hemopoietic Stem Cell Engraftment and Function
J. Immunol.,
January 1, 2008;
180(1):
49 - 57.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhang, I. Hirschler-Laszkiewicz, Q. Tong, K. Conrad, S.-C. Sun, L. Penn, D. L. Barber, R. Stahl, D. J. Carey, J. Y. Cheung, et al.
TRPM2 is an ion channel that modulates hematopoietic cell death through activation of caspases and PARP cleavage
Am J Physiol Cell Physiol,
April 1, 2006;
290(4):
C1146 - C1159.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jamaluddin, S. Choudhary, S. Wang, A. Casola, R. Huda, R. P. Garofalo, S. Ray, and A. R. Brasier
Respiratory Syncytial Virus-Inducible BCL-3 Expression Antagonizes the STAT/IRF and NF-{kappa}B Signaling Pathways by Inducing Histone Deacetylase 1 Recruitment to the Interleukin-8 Promoter
J. Virol.,
December 15, 2005;
79(24):
15302 - 15313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wessells, M. Baer, H. A. Young, E. Claudio, K. Brown, U. Siebenlist, and P. F. Johnson
BCL-3 and NF-{kappa}B p50 Attenuate Lipopolysaccharide-induced Inflammatory Responses in Macrophages
J. Biol. Chem.,
November 26, 2004;
279(48):
49995 - 50003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Chu, Q. Tong, J. Y. Cheung, J. Wozney, K. Conrad, V. Mazack, W. Zhang, R. Stahl, D. L. Barber, and B. A. Miller
Interaction of TRPC2 and TRPC6 in Erythropoietin Modulation of Calcium Influx
J. Biol. Chem.,
March 12, 2004;
279(11):
10514 - 10522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Kurland, D. W. Voehringer, and R. E. Meyn
The MEK/ERK Pathway Acts Upstream of NF{kappa}B1 (p50) Homodimer Activity and Bcl-2 Expression in a Murine B-Cell Lymphoma Cell Line: MEK INHIBITION RESTORES RADIATION-INDUCED APOPTOSIS
J. Biol. Chem.,
August 22, 2003;
278(34):
32465 - 32470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Watanabe, S. Wachi, and T. Fujita
Identification and Characterization of BCL-3-binding Protein: IMPLICATIONS FOR TRANSCRIPTION AND DNA REPAIR OR RECOMBINATION
J. Biol. Chem.,
July 3, 2003;
278(28):
26102 - 26110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Ebner, A. Bandion, B. R. Binder, R. de Martin, and J. A. Schmid
GMCSF activates NF-{kappa}B via direct interaction of the GMCSF receptor with I{kappa}B kinase {beta}
Blood,
July 1, 2003;
102(1):
192 - 199.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Zhang, X. Chu, Q. Tong, J. Y. Cheung, K. Conrad, K. Masker, and B. A. Miller
A Novel TRPM2 Isoform Inhibits Calcium Influx and Susceptibility to Cell Death
J. Biol. Chem.,
April 25, 2003;
278(18):
16222 - 16229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Chu, J. Y. Cheung, D. L. Barber, L. Birnbaumer, L. I. Rothblum, K. Conrad, V. Abrasonis, Y.-m. Chan, R. Stahl, D. J. Carey, et al.
Erythropoietin Modulates Calcium Influx through TRPC2
J. Biol. Chem.,
September 6, 2002;
277(37):
34375 - 34382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Nakamura, R. Ouchida, T. Kodama, T. Kawashima, Y. Makino, N. Yoshikawa, S. Watanabe, C. Morimoto, T. Kitamura, and H. Tanaka
Cytokine Receptor Common beta Subunit-mediated STAT5 Activation Confers NF-kappa B Activation in Murine proB Cell Line Ba/F3 Cells
J. Biol. Chem.,
February 15, 2002;
277(8):
6254 - 6265.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. D. Westerheide, M. W. Mayo, V. Anest, J. L. Hanson, and A. S. Baldwin Jr.
The Putative Oncoprotein Bcl-3 Induces Cyclin D1 To Stimulate G1 Transition
Mol. Cell. Biol.,
December 15, 2001;
21(24):
8428 - 8436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Cha-Molstad, A. Agrawal, D. Zhang, D. Samols, and I. Kushner
The Rel Family Member P50 Mediates Cytokine-Induced C-Reactive Protein Expression by a Novel Mechanism
J. Immunol.,
October 15, 2000;
165(8):
4592 - 4597.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rebollo, L. Dumoutier, J.-C. Renauld, A. Zaballos, V. Ayllón, and C. Martínez-A.
Bcl-3 Expression Promotes Cell Survival following Interleukin-4 Deprivation and Is Controlled by AP1 and AP1-Like Transcription Factors
Mol. Cell. Biol.,
May 15, 2000;
20(10):
3407 - 3416.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. F. Romano, A. Lamberti, R. Bisogni, C. Garbi, A. M. Pagnano, P. Auletta, P. Tassone, M. C. Turco, and S. Venuta
Amifostine Inhibits Hematopoietic Progenitor Cell Apoptosis by Activating NF-kappa B/Rel Transcription Factors
Blood,
December 15, 1999;
94(12):
4060 - 4066.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Y. Na, J.-E. Choi, H.-J. Kim, B. H. Jhun, Y.-C. Lee, and J. W. Lee
Bcl3, an Ikappa B Protein, Stimulates Activating Protein-1 Transactivation and Cellular Proliferation
J. Biol. Chem.,
October 1, 1999;
274(40):
28491 - 28496.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Miller, D. L. Barber, L. L. Bell, B. K. Beattie, M.-Y. Zhang, B. G. Neel, M. Yoakim, L. I. Rothblum, and J. Y. Cheung
Identification of the Erythropoietin Receptor Domain Required for Calcium Channel Activation
J. Biol. Chem.,
July 16, 1999;
274(29):
20465 - 20472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Brasier, M. Lu, T. Hai, Y. Lu, and I. Boldogh
NF-kappa B-inducible BCL-3 Expression Is an Autoregulatory Loop Controlling Nuclear p50/NF-kappa B1 Residence
J. Biol. Chem.,
August 17, 2001;
276(34):
32080 - 32093.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
