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Blood, Vol. 92 No. 4 (August 15), 1998:
pp. 1277-1286
Outside-In Signaling of Soluble and Solid-Phase Fibrinogen Through
Integrin  IIb 3 Is Different and Cooperative With Each Other in
a Megakaryoblastic Leukemia Cell Line, CMK
Yumi Tohyama,
Kaoru Tohyama,
Misao Tsubokawa,
Momoyo Asahi,
Yataro Yoshida, and
Hirohei Yamamura
From the Department of Biochemistry, Kobe University School of
Medicine, Kobe, Japan; the Department of Laboratory Medicine and
Clinical Sciences, and Department of Hematology/Oncology, Graduate
School of Medicine, Kyoto University, Kyoto, Japan; the Department of
Otorhinolaryngology, Fukui Medical School, Fukui, Japan; and the
Department of Biochemistry, Fukui Prefectural University, Fukui, Japan.
The function and the outside-in signaling pathways of  IIb 3
were examined in relation to cell adhesion using a megakaryoblastic leukemia cell line, CMK. After 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment, the cells adhered to the culture plate and underwent megakaryocytic differentiation with expression of IIb3. Binding of soluble fibrinogen to the cells via IIb3 was dependent on cell
adhesion. Cell detaching reduced the affinity of this integrin for
soluble fibrinogen, although its surface expression was almost unchanged. In contrast, detached cells became tightly adherent to the
fibrinogen-coated plate (solid-phase fibrinogen). The same ligand,
fibrinogen, present either in soluble or solid-phase form, triggered
differential signaling pathways mediated by IIb3. By the
stimulation with soluble fibrinogen, Syk was tyrosine-phosphorylated but FAK was dephosphorylated, whereas solid-phase fibrinogen promptly caused tyrosine phosphorylation of FAK followed by delayed
phosphorylation of Syk. In addition, the binding of soluble fibrinogen
to the cells adherent to fibrinogen-coated plate resulted in tyrosine phosphorylation of integrin 3 and a complex formation of integrin 3 with Syk. This implies the cooperation of both soluble and solid-phase fibrinogen-mediated signaling pathways.
© 1998 by The American Society of Hematology.
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