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Blood, Vol. 92 No. 4 (August 15), 1998:
pp. 1308-1316
Transcription Factor B-Cell-Specific Activator Protein
(BSAP) Is Differentially Expressed in B Cells and in Subsets of B-Cell
Lymphomas
Laszlo Krenacs,
Andreas W. Himmelmann,
Leticia Quintanilla-Martinez,
Thierry Fest,
Agostino Riva,
Axel Wellmann,
Eniko Bagdi,
John H. Kehrl,
Elaine S. Jaffe, and
Mark Raffeld
From the Hematopathology Section, Laboratory of Pathology, National
Cancer Institute and the B cell Molecular Immunology Section,
Laboratory of Immunoregulation, National Institute of Allergy and
Infectious Diseases, National Institutes of Health, Bethesda, MD; and
the Department of Pathology, Albert Szent-Gyorgyi Medical University,
Szeged, Hungary.
The paired box containing gene PAX-5 encodes the
transcription factor BSAP (B-cell-specific activator protein), which
plays a key role in B-lymphocyte development. Despite its known
involvement in a rare subtype of non-Hodgkin's lymphoma (NHL), a
detailed examination of BSAP expression in NHL has not been previously reported. In this study, we analyzed normal and malignant lymphoid tissues and cell lines, including 102 cases of B-cell NHL, 23 cases of
T- and null-cell NHL, and 18 cases of Hodgkin's disease. Normal
lymphoid tissues showed strong nuclear BSAP expression in mantle zone B
cells, less intense reactivity in follicular center B cells, and no
expression in cells of the T-cell-rich zones. Monocytoid B cells
showed weak expression, whereas plasma cells and extrafollicular large
transformed B cells were negative. Of the 102 B-cell NHLs, 83 (81%)
demonstrated BSAP expression. All of the 13 (100%) B-cell chronic
lymphocytic leukemias (B-CLLs), 21 of (100%) mantle cells (MCLs), and
20 of 21 (95%) follicular lymphomas (FLs) were positive. Moderate
staining intensities were found in most B-CLL and FL cases, whereas
most MCLs showed strong reactions, paralleling the strong reactivity of
nonmalignant mantle cells. Eight of 12 (67%) marginal zone lymphoma
cases showed negative or low BSAP levels, and 17 of 24 (71%) large
B-cell lymphomas displayed moderate to strong expression. None of the
23 T- and null-cell lymphomas reacted with the BSAP antisera, whereas
in Hodgkin's disease, 2 of 4 (50%) nodular lymphocytic predominance and 5 of 14 (36%) classical cases showed weak nuclear or nucleolar BSAP reactions in a fraction of the tumor cells. Western blot analysis
showed a 52-kD BSAP band in B-cell lines, but not in non-B-cell or
plasma cell lines. We conclude that BSAP expression is largely
restricted to lymphomas of B-cell lineage and that BSAP expression
varies in B-cell subsets and subtypes of B-cell NHL. The high levels of
BSAP, especially those found in large-cell lymphomas and in some
follicular lymphomas, may be a consequence of deregulated gene
expression and suggest a possible involvement of PAX-5 in
certain B-cell malignancies.
This is a US government work. There are no restrictions on its use.
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