|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 92 No. 4 (August 15), 1998:
pp. 1374-1383
Characterization of a Novel Human Natural Killer-Cell Line (NK-YS)
Established From Natural Killer Cell Lymphoma/Leukemia Associated
With Epstein-Barr Virus Infection
Junjiro Tsuchiyama,
Tadashi Yoshino,
Masaharu Mori,
Eisaku Kondoh,
Takeshi Oka,
Tadaatsu Akagi,
Akio Hiraki,
Hiroyuki Nakayama,
Akira Shibuya,
Yuxiang Ma,
Teruyuki Kawabata,
Shigeru Okada, and
Mine Harada
From the Second Department of Internal Medicine, the Second
Department of Pathology, the Department of Immunology, and the First
Department of Pathology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama, Japan and the Faculty of Health and Welfare
Science, Okayama Prefectural University, Soja, Japan.
A novel cell line was established from a patient with a
leukemic-state nasal angiocentric natural killer (NK) cell lymphoma with systemic skin infiltration. The morphology of the leukemic cells
was large-granular-lymphocyte (LGL), and their immunophenotype was
CD2+, CD3 , CD5+,
CD7+, CD16, CD56+, and
CD57. The presence of Epstein-Barr viral (EBV) genome
was shown in specimens from the patient's nose, skin, and
peripheral blood by in situ hybridization using an EBV-encoded
small RNA-1 probe or by Southern blotting using a terminal-repeat probe
of the EBV genome. Leukemic cells were cocultured with a mouse stromal
cell line (SPY3-2) in the presence of 100 U/mL recombinant human
interleukin-2 and a novel stromal cell-independent cell line, NK-YS,
was established. The NK-YS cells showed LGL morphology and expressed
surface CD2, CD5, CD7, CD25, CD56, and CD95. The NK-YS cells retained
cytotoxicity against K562 and Jurkat cells. A Southern blotting using a
terminal-repeat probe of EBV showed that NK-YS and fresh leukemic cells
had a clonal EBV genome, whereas the T-cell receptor  and  chain
genes of NK-YS were not rearranged. In an immunocytochemical analysis, the NK-YS cells showed a type-II latent infection of EBV. The NK-YS cells preserved the original characteristics of NK cell lymphoma/leukemia and will be a useful tool for the study of
biological characteristics of EBV-associated nasal angiocentric
NK cell lymphoma/leukemia.
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Shen, W.-Y. Au, K.-Y. Wong, N. Shimizu, J. Tsuchiyama, Y.-L. Kwong, R. H. Liang, and G. Srivastava
Cell death by bortezomib-induced mitotic catastrophe in natural killer lymphoma cells
Mol. Cancer Ther.,
December 1, 2008;
7(12):
3807 - 3815.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. P. Thompson and R. Kurzrock
Epstein-Barr Virus and Cancer
Clin. Cancer Res.,
February 1, 2004;
10(3):
803 - 821.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Oka, T. Yoshino, K. Hayashi, N. Ohara, T. Nakanishi, Y. Yamaai, A. Hiraki, C. A. Sogawa, E. Kondo, N. Teramoto, et al.
Reduction of Hematopoietic Cell-Specific Tyrosine Phosphatase SHP-1 Gene Expression in Natural Killer Cell Lymphoma and Various Types of Lymphomas/Leukemias : Combination Analysis with cDNA Expression Array and Tissue Microarray
Am. J. Pathol.,
October 1, 2001;
159(4):
1495 - 1505.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Nagata, A. Konno, N. Kimura, Y. Zhang, M. Kimura, A. Demachi, T. Sekine, K. Yamamoto, and N. Shimizu
Characterization of novel natural killer (NK)-cell and {gamma}{delta} T-cell lines established from primary lesions of nasal T/NK-cell lymphomas associated with the Epstein-Barr virus
Blood,
February 1, 2001;
97(3):
708 - 713.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
