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Blood, Vol. 92 No. 4 (August 15), 1998:
pp. 1423-1431
Macrophage Lineage Cells in Inflammation: Characterization by
Colony-Stimulating Factor-1 (CSF-1) Receptor (c-Fms), ER-MP58, and
ER-MP20 (Ly-6C) Expression
James Chan,
Pieter J.M. Leenen,
Ivan Bertoncello,
Shin-Ichi Nishikawa, and
John A. Hamilton
From the Inflammation Research Centre, University of Melbourne,
Department of Medicine, The Royal Melbourne Hospital, Parkville,
Victoria, Australia; the Department of Immunology, Erasmus University,
Rotterdam, The Netherlands; the Peter MacCallum Cancer Institute, East
Melbourne, Victoria, Australia; and the Department of Molecular
Genetics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto,
Japan.
Macrophage populations resident in tissues and at sites of
inflammation are heterogeneous and with local proliferation sometimes evident. Using the convenient murine peritoneal cavity as an
inflammation model, the appearance of macrophage lineage cells was
followed with time in both thioglycollate- and sodium periodate-induced exudates. The cells were characterized by their proliferative response
in vitro in response to colony-stimulating factor-1 (CSF-1) (or
macrophage colony-stimulating factor [M-CSF]), particularly by their
ability to form colonies in agar, in combination with flow cytometry
(surface marker expression and forward and side scatter
characteristics). We propose that c-Fms (CSF-1 receptor), unlike other
markers, is a uniformly expressed and specific marker suitable for the
detection of macrophage-lineage cells in tissues, both in the steady
state and after the initiation of an inflammatory reaction. It was
shown that the bone marrow myeloid precursor markers, ER-MP58 and
ER-MP20 (Ly-6C), but not ER-MP12 (PECAM-1), are expressed by a high
proportion of macrophage-lineage cells in the inflamed peritoneum. The
macrophage colony-forming cells (M-CFCs) in a 16-hour
thioglycollate-induced exudate were phenotyped as
c-Fms+ERMP12 20+58+,
properties consistent with their being more mature than bone marrow
M-CFCs. It is proposed that ER-MP58, as well as ER-MP20, may be a
useful marker for distinguishing inflammatory macrophage-lineage cells
from the majority of those residing normally in tissues.
© 1998 by The American Society of Hematology.
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