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Blood, Vol. 92 No. 6 (September 15), 1998:
pp. 1878-1886
RAPID COMMUNICATION
Improved Gene Transfer Into Baboon Marrow Repopulating Cells Using
Recombinant Human Fibronectin Fragment CH-296 in Combination With
Interleukin-6, Stem Cell Factor, FLT-3 Ligand, and Megakaryocyte
Growth and Development Factor
Hans-Peter Kiem,
Robert G. Andrews,
Julia Morris,
Laura Peterson,
Scott Heyward,
James M. Allen,
John E.J. Rasko,
Jennifer Potter, and
A. Dusty Miller
From the Clinical Research and Molecular Medicine Divisions, Fred
Hutchinson Cancer Research Center, Seattle, WA; the Departments of
Medicine, Pediatrics, and Pathology, University of Washington School of
Medicine, Seattle, WA; and the University of Washington Regional
Primate Research Center, Seattle, WA.
We have used a competitive repopulation assay in baboons to develop
improved methods for hematopoietic stem cell transduction and have
previously shown increased gene transfer into baboon marrow
repopulating cells using a gibbon ape leukemia virus (GALV)-pseudotype retroviral vector (Kiem et al, Blood 90:4638, 1997). In this
study using GALV-pseudotype vectors, we examined additional variables that have been reported to increase gene transfer into hematopoietic progenitor cells in culture for their ability to increase gene transfer
into baboon hematopoietic repopulating cells. Baboon marrow was
harvested after in vivo administration (priming) of stem cell factor
(SCF) and granulocyte colony-stimulating factor (G-CSF). CD34-enriched
marrow cells were divided into two equal fractions to directly compare
transduction efficiencies under different gene transfer conditions.
Transduction by either incubation with retroviral vectors on
CH-296-coated flasks or by cocultivation on vector-producing cells was
studied in five animals; in one animal, transduction on CH-296 was
compared with transduction on bovine serum albumin (BSA)-coated flasks.
The highest level of gene transfer was obtained after 24 hours of
prestimulation followed by 48 hours of incubation on CH-296 in
vector-containing medium in the presence of multiple hematopoietic
growth factors (interleukin-6, stem cell factor, FLT-3 ligand, and
megakaryocyte growth and development factor). Using these conditions,
up to 20% of peripheral blood and marrow cells contained vector
sequences for more than 20 weeks, as determined by both polymerase
chain reaction and Southern blot analysis. Gene transfer rates were higher for cells transduced on CH-296 as compared with BSA or cocultivation. In one animal, we have used a vector expressing a cell
surface protein (human placental alkaline phosphatase) and have
detected 10% and 5% of peripheral blood cells expressing the
transduced gene 2 and 4 weeks after transplantation as measured by flow
cytometry. In conclusion, the conditions described here have resulted
in gene transfer rates that will allow detection of transduced cells by
flow cytometry to facilitate the evaluation of gene expression. The
levels of gene transfer obtained with these conditions suggest the
potential for therapeutic efficacy in diseases affecting the
hematopoietic system.
© 1998 by The American Society of Hematology.
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|
|
|
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|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
|
J. L. Abkowitz, D. Golinelli, D. E. Harrison, and P. Guttorp
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|
|
|
|
|
|
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|
|
|
|
|
|
|
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96(4):
1206 - 1214.
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[PDF]
|
|
|
|
|
|
|
D. W. Emery, E. Yannaki, J. Tubb, and G. Stamatoyannopoulos
A chromatin insulator protects retrovirus vectors from chromosomal position effects
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July 19, 2000;
(2000)
160159597.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. J. Kim, J. F. Tisdale, T. Wu, M. Takatoku, S. E. Sellers, P. Zickler, M. E. Metzger, B. A. Agricola, J. D. Malley, I. Kato, et al.
Many multipotential gene-marked progenitor or stem cell clones contribute to hematopoiesis in nonhuman primates
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July 1, 2000;
96(1):
1 - 8.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Barquinero, J. C. Segovia, M. Ramirez, A. Limon, G. Guenechea, T. Puig, J. Briones, J. Garcia, and J. A. Bueren
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95(10):
3085 - 3093.
[Abstract]
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[PDF]
|
|
|
|
|
|
|
D. A. Persons and A. W. Nienhuis
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May 9, 2000;
97(10):
5022 - 5024.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Schiedlmeier, K. Kuhlcke, H. G. Eckert, C. Baum, W. J. Zeller, and S. Fruehauf
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Blood,
February 15, 2000;
95(4):
1237 - 1248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Donahue, R. P. Wersto, J. A. Allay, B. A. Agricola, M. E. Metzger, A. W. Nienhuis, D. A. Persons, and B. P. Sorrentino
High levels of lymphoid expression of enhanced green fluorescent protein in nonhuman primates transplanted with cytokine-mobilized peripheral blood CD34+ cells
Blood,
January 15, 2000;
95(2):
445 - 452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Williams, A. W. Nienhuis, R. G. Hawley, and F. O. Smith
Gene Therapy 2000
Hematology,
January 1, 2000;
2000(1):
376 - 393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Rosenzweig, T.J. MacVittie, D. Harper, D. Hempel, R.L. Glickman, R.P. Johnson, A.M. Farese, N. Whiting-Theobald, G.F. Linton, G. Yamasaki, et al.
Efficient and Durable Gene Marking of Hematopoietic Progenitor Cells in Nonhuman Primates After Nonablative Conditioning
Blood,
October 1, 1999;
94(7):
2271 - 2286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Goerner, B. Bruno, P. A. McSweeney, G. Buron, R. Storb, and H.-P. Kiem
The Use of Granulocyte Colony-Stimulating Factor During Retroviral Transduction on Fibronectin Fragment CH-296 Enhances Gene Transfer Into Hematopoietic Repopulating Cells in Dogs
Blood,
October 1, 1999;
94(7):
2287 - 2292.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Dinauer, L. L. Li, H. Bjorgvinsdottir, C. Ding, and N. Pech
Long-Term Correction of Phagocyte NADPH Oxidase Activity by Retroviral-Mediated Gene Transfer in Murine X-Linked Chronic Granulomatous Disease
Blood,
August 1, 1999;
94(3):
914 - 922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. H. Cowan, J. A. Moscow, H. Huang, J. A. Zujewski, J. O'Shaughnessy, B. Sorrentino, K. Hines, C. Carter, E. Schneider, G. Cusack, et al.
Paclitaxel Chemotherapy after Autologous Stem-Cell Transplantation and Engraftment of Hematopoietic Cells Transduced with a Retrovirus Containing the Multidrug Resistance Complementary DNA (MDR1) in Metastatic Breast Cancer Patients
Clin. Cancer Res.,
July 1, 1999;
5(7):
1619 - 1628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. B. Kohn, G. Bauer, C. R. Rice, J.C. Rothschild, D. A. Carbonaro, P. Valdez, Q.-l. Hao, C. Zhou, I. Bahner, K. Kearns, et al.
A Clinical Trial of Retroviral-Mediated Transfer of a rev-Responsive Element Decoy Gene Into CD34+ Cells From the Bone Marrow of Human Immunodeficiency Virus-1-Infected Children
Blood,
July 1, 1999;
94(1):
368 - 371.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. MacNeill, H. Hanenberg, K. E. Pollok, J. C. M. van der Loo, M. F. A. Bierhuizen, G. Wagemaker, and D. A. Williams
Simultaneous Infection with Retroviruses Pseudotyped with Different Envelope Proteins Bypasses Viral Receptor Interference Associated with Colocalization of gp70 and Target Cells on Fibronectin CH-296
J. Virol.,
May 1, 1999;
73(5):
3960 - 3967.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. S. Case, M. A. Price, C. T. Jordan, X. J. Yu, L. Wang, G. Bauer, D. L. Haas, D. Xu, R. Stripecke, L. Naldini, et al.
Stable transduction of quiescent CD34+CD38- human hematopoietic cells by HIV-1-based lentiviral vectors
PNAS,
March 16, 1999;
96(6):
2988 - 2993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Miyoshi, K. A. Smith, D. E. Mosier, I. M. Verma, and B. E. Torbett
Transduction of Human CD34+ Cells That Mediate Long-Term Engraftment of NOD/SCID Mice by HIV Vectors
Science,
January 29, 1999;
283(5402):
682 - 686.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Wolgamot, J. E. J. Rasko, and A. D. Miller
Retrovirus Packaging Cells Expressing the Mus dunni Endogenous Virus Envelope Facilitate Transduction of CHO and Primary Hematopoietic Cells
J. Virol.,
December 1, 1998;
72(12):
10242 - 10245.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Emery, E. Yannaki, J. Tubb, and G. Stamatoyannopoulos
A chromatin insulator protects retrovirus vectors from chromosomal position effects
PNAS,
August 1, 2000;
97(16):
9150 - 9155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
