Blood, Vol. 92 No. 6 (September 15), 1998:
pp. 2113-2117
Allelotype Analysis of Adult T-Cell Leukemia
Yoshihiro Hatta,
Yasuaki Yamada,
Masao Tomonaga,
Jonathan W. Said,
Isao Miyosi, and
H. Phillip Koeffler
From the Division of Hematology/Oncology, Cedars-Sinai Research
Institute, UCLA School of Medicine, Los Angeles, CA; the Deparment of
Laboratoy Medicine and the Department of Hematology, Nagasaki
University School of Medicine, Nagasaki, Japan; the Department of
Pathology, Center for the Health Science, UCLA School of Medicine, Los
Angeles, CA; and the Department of Medicine, Kochi Medical School,
Kochi, Japan.
Allelotype analysis of adult T-cell leukemia (ATL) was undertaken
for the first time to identify chromosomal loci relevant to the
development of acute/lymphomatous ATL. Loss of heterozygosity (LOH) was
screened using 94 highly polymorphic microsatellite markers,
distributed among all nonacrocentric, autosomal chromosomes. In each of
the 22 cases, DNA obtained from their leukemic cells in
acute/lymphomatous phase was compared with their constitutional DNA
from mononuclear cells in chronic or remission phase. Allelic losses of
at least on one chromosome arm occurred in 91% of the cases (20 individuals). Among 39 chromosome arms, allelic losses were observed on
31 arms at least for one sample. A high frequency of allelic loss
(>30%) was seen on chromosome arms 6q (41%) and 17p (48%). The
mean fractional allelic loss (FAL) was 0.109. These findings suggest
that a novel tumor suppressor gene on chromosome arm 6q, as well as the
p53 gene on chromosome arm 17p, probably have an important role in the
development of acute/lymphomatous ATL.
© 1998 by The American Society of Hematology.
