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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kritzik, M. Articles by Kunicki, T. J. Search for Related Content PubMed PubMed Citation Articles by Kritzik, M. Articles by Kunicki, T. J. Related Collections Hemostasis, Thrombosis, and Vascular Biology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 92 No. 7 (October 1), 1998: pp. 2382-2388 Nucleotide Polymorphisms in the 2 Gene Define Multiple Alleles That Are Associated With Differences in Platelet 21 Density Marcie Kritzik, Brian Savage, Diane J. Nugent, Sentot Santoso, Zaverio M. Ruggeri, and Thomas J. Kunicki From the Roon Research Center for Arteriosclerosis and Thrombosis, Division of Experimental Hemostasis and Thrombosis of the Department of Molecular and Experimental Medicine and The Department of Vascular Biology, The Scripps Research Institute, La Jolla; the Children's Hospital of Orange County, Orange, CA; and the Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany. Three allelic differences in the 2 gene are associated with expression levels of the 21 integrin on the platelet surface. We have previously defined two linked silent polymorphisms in the 2 gene coding region at nucleotides 807 (C or T) and 873 (G or A). We have now identified one rarer nucleotide polymorphism in the coding region at nucleotide 837 (T or C) and four additional linked polymorphisms within the introns that flank these coding sequences. Moreover, we have determined that the alloantigenic Br polymorphism, which resides in a distal coding region at nucleotide 1648, is also linked to the 837 polymorphism. Thus, three 2 gene alleles, defined by eight nucleotide polymorphisms, have now been discovered. Allele 1 (807T/837T/873A/Brb) is associated with increased levels of 21; allele 2 (807C/837T/873G/Brb) and allele 3 (807C/837C/873G/Bra) are each associated with lower levels of 21. Finally, we also show here that the rate of platelet attachment to type I collagen in whole blood under conditions of high shear rate (1,500/s) is proportional to the density of 21 receptors on the platelet surface. Thus, the density of platelet 21 could have an important impact on platelet adhesion to collagen in whole blood and therefore on platelet function in vivo, contributing to an increased risk of thrombosis or to bleeding in relevant disease states. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? 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