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Blood, Vol. 92 No. 7 (October 1), 1998:
pp. 2410-2420
Age-Related Decline of Perforin Expression in Human Cytotoxic T
Lymphocytes and Natural Killer Cells
Daniel Rukavina,
Gordana Laskarin,
Gordana Rubesa,
Natasa Strbo,
Ivica Bedenicki,
Darko Manestar,
Mario Glavas,
Stephen E. Christmas, and
Eckhard R. Podack
From the Department of Physiology and Immunology, Medical Faculty,
University of Rijeka, Rijeka; the Department of Otorhinolaringology,
Clinical Hospital Center, University of Rijeka, Rijeka, Croatia; the
Clinical Hospital Center Pula, Pula, Croatia; the
Department of Immunology, University of Liverpool, Liverpool, UK; and
the Department of Microbiology and Immunology, University of Miami,
Miami, FL.
In this study a flow cytometric technique for detecting cytoplasmic
perforin (P) has been used to quantify age-related changes in perforin
expression in human peripheral blood lymphocytes (PBL). Proportions of
P+ lymphocytes increased after birth, but declined
rapidly after the age of 70 years. This was true for both T cells and
CD16+ and CD56+ natural killer (NK) cells.
Children showed in addition to high levels of perforin positive
CD8+ cells a much higher proportion of
CD4+P+ cells than the other age groups. In
elderly individuals there was also a highly significant reduction in
mean levels of perforin per cell as compared with all other groups
(P < .05 to .001). Adult women had consistently higher mean
levels of perforin per cell than adult men for all P+
cell phenotypes. Functional tests clearly showed the deficiency in
early spontaneous cytotoxic potential of PBL from elderly persons due
to relative P deficiency, which can be corrected by stimulation of
cytolytic cells with target cells and interleukin-2 (IL-2). The
deficiency in cytolytic activity on the contact with target cells may
have implications for antiviral and antitumor immunity in elderly
persons.
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