|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 92 No. 7 (October 1), 1998:
pp. 2556-2570
Nonobese Diabetic/Severe Combined Immunodeficiency (NOD/SCID)
Mouse as a Model System to Study the Engraftment and Mobilization
of Human Peripheral Blood Stem Cells
Johannes C.M. van der Loo,
Helmut Hanenberg,
Ryan J. Cooper,
F.-Y. Luo,
Emmanuel N. Lazaridis, and
David A. Williams
From the Department of Pediatrics, Section of Hematology/Oncology,
Herman B Wells Center for Pediatric Research, the Stem Cell Laboratory,
Cancer Research Building, and the Division of Biostatistics,
Regenstrief Institute for Health Care, Indiana University School of
Medicine, Indianapolis, IN; and Howard Hughes Medical Institute,
Indiana University School of Medicine, Indianapolis, IN.
Mobilized CD34+ cells from human peripheral blood (PB)
are increasingly used for hematopoietic stem-cell transplantation.
However, the mechanisms involved in the mobilization of human
hematopoietic stem and progenitor cells are largely unknown. To study
the mobilization of human progenitor cells in an experimental animal
model in response to different treatment regimens, we injected
intravenously a total of 92 immunodeficient nonobese diabetic/severe
combined immunodeficiency (NOD/SCID) mice with various numbers of
granulocyte colony-stimulating factor (G-CSF) -mobilized
CD34+ PB cells (ranging from 2 to 50 × 106
cells per animal). Engraftment of human cells was detectable for up to
6.5 months after transplantation and, depending on the number of cells
injected, reached as high as 96% in the bone marrow (BM), displaying
an organ-specific maturation pattern of T- and B-lymphoid and myeloid
cells. Among the different mobilization regimens tested, human
clonogenic cells could be mobilized from the BM into the PB (P
= .019) with a high or low dose of human G-CSF, alone or in
combination with human stem-cell factor (SCF), with an average increase
of 4.6-fold over control. Therefore, xenotransplantation of human cells
in NOD/SCID mice will provide a basis to further study the mechanisms
of mobilization and the biology of the mobilized primitive human
hematopoietic cell.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
K. Yamamura, K. Ohishi, M. Masuya, E. Miyata, Y. Sugimoto, S. Nakamura, A. Fujieda, H. Araki, and N. Katayama
Ex Vivo Culture of Human Cord Blood Hematopoietic Stem/Progenitor Cells Adversely Influences Their Distribution to Other Bone Marrow Compartments After Intra-Bone Marrow Transplantation
Stem Cells,
February 1, 2008;
26(2):
543 - 549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wu, J. Vieira, N. Fiore, P. Banerjee, M. Sieburg, R. Rochford, W. Harrington Jr, and G. Feuer
KSHV/HHV-8 infection of human hematopoietic progenitor (CD34+) cells: persistence of infection during hematopoiesis in vitro and in vivo
Blood,
July 1, 2006;
108(1):
141 - 151.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. D. Shultz, B. L. Lyons, L. M. Burzenski, B. Gott, X. Chen, S. Chaleff, M. Kotb, S. D. Gillies, M. King, J. Mangada, et al.
Human Lymphoid and Myeloid Cell Development in NOD/LtSz-scid IL2R{gamma}null Mice Engrafted with Mobilized Human Hemopoietic Stem Cells
J. Immunol.,
May 15, 2005;
174(10):
6477 - 6489.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Xu, E. Bruno, J. Chao, H. Ni, V. Lindgren, R. Nunez, N. Mahmud, G. Finazzi, S. M. Fruchtman, U. Popat, et al.
The constitutive mobilization of bone marrow-repopulating cells into the peripheral blood in idiopathic myelofibrosis
Blood,
February 15, 2005;
105(4):
1699 - 1705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Samira, C. Ferrand, A. Peled, A. Nagler, Y. Tovbin, H. Ben-Hur, N. Taylor, A. Globerson, and T. Lapidot
Tumor Necrosis Factor Promotes Human T-Cell Development in Nonobese Diabetic/Severe Combined Immunodeficient Mice
Stem Cells,
November 1, 2004;
22(6):
1085 - 1100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Monaco, M. Konopleva, M. Munsell, C. Leysath, R.-Y. Wang, C. E. Jackson, M. Korbling, E. Estey, J. Belmont, and M. Andreeff
Engraftment of Acute Myeloid Leukemia in NOD/SCID Mice Is Independent of CXCR4 and Predicts Poor Patient Survival
Stem Cells,
March 1, 2004;
22(2):
188 - 201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Brenner, N. L. Whiting-Theobald, G. F. Linton, K. L. Holmes, M. Anderson-Cohen, P. F. Kelly, E. F. Vanin, A. M. Pilon, D. M. Bodine, M. E. Horwitz, et al.
Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/{beta}2-microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells
Blood,
October 15, 2003;
102(8):
2789 - 2797.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. Kalberer, U. Siegler, and A. Wodnar-Filipowicz
Human NK cell development in NOD/SCID mice receiving grafts of cord blood CD34+ cells
Blood,
July 1, 2003;
102(1):
127 - 135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Saito, Y. Kametani, K. Hozumi, N. Mochida, K. Ando, M. Ito, T. Nomura, Y. Tokuda, H. Makuuchi, T. Tajima, et al.
The in vivo development of human T cells from CD34+ cells in the murine thymic environment
Int. Immunol.,
October 1, 2002;
14(10):
1113 - 1124.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Wagner, S. M. Guichard, R. A. Burger, C. L. Morton, C. M. Straign, R. A. Ashmun, L. C. Harris, P. J. Houghton, P. M. Potter, and M. K. Danks
Efficacy and Toxicity of a Virus-directed Enzyme Prodrug Therapy Purging Method: Preclinical Assessment and Application to Bone Marrow Samples from Neuroblastoma Patients
Cancer Res.,
September 1, 2002;
62(17):
5001 - 5007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yahata, K. Ando, Y. Nakamura, Y. Ueyama, K. Shimamura, N. Tamaoki, S. Kato, and T. Hotta
Functional Human T Lymphocyte Development from Cord Blood CD34+ Cells in Nonobese Diabetic/Shi-scid, IL-2 Receptor {gamma} Null Mice
J. Immunol.,
July 1, 2002;
169(1):
204 - 209.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bazin, E. Aubin, L. Boyer, I. St-Amour, C. Roberge, and R. Lemieux
Functional in vivo characterization of human monoclonal anti-D in NOD-scid mice
Blood,
February 15, 2002;
99(4):
1267 - 1272.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. D. Lewis, G. Almeida-Porada, J. Du, I. R. Lemischka, K. A. Moore, E. D. Zanjani, and C. M. Verfaillie
Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system
Blood,
June 1, 2001;
97(11):
3441 - 3449.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Perez, H. M. Rinder, C. Wang, J. B. Tracey, N. Maun, and D. S. Krause
Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production
Blood,
March 15, 2001;
97(6):
1635 - 1643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, J. H. F. Falkenburg, X. Tong, W. A. Noort, R. Breese, D. Heilman, H. Kanhai, C. M. Orschell-Traycoff, and E. F. Srour
Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice
Blood,
September 15, 2000;
96(6):
2100 - 2107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Kollet, A. Peled, T. Byk, H. Ben-Hur, D. Greiner, L. Shultz, and T. Lapidot
beta 2 Microglobulin-deficient (B2mnull) NOD/SCID mice are excellent recipients for studying human stem cell function
Blood,
May 15, 2000;
95(10):
3102 - 3105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Schiedlmeier, K. Kuhlcke, H. G. Eckert, C. Baum, W. J. Zeller, and S. Fruehauf
Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice
Blood,
February 15, 2000;
95(4):
1237 - 1248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Robin, F. Pflumio, W. Vainchenker, and L. Coulombel
Identification of Lymphomyeloid Primitive Progenitor Cells in Fresh Human Cord Blood and in the Marrow of Nonobese Diabetic-Severe Combined Immunodeficient (NOD-SCID) Mice Transplanted with Human CD34+ Cord Blood Cells
J. Exp. Med.,
May 17, 1999;
189(10):
1601 - 1610.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
