|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 92 No. 8 (October 15), 1998:
pp. 2641-2649
RAPID COMMUNICATION
Cell Cycle-Related Changes in Repopulating Capacity of Human Mobilized
Peripheral Blood CD34+ Cells in Non-Obese Diabetic/Severe
Combined Immune-Deficient Mice
André Gothot,
Johannes C.M. van der Loo,
D. Wade Clapp, and
Edward F. Srour
From the Division of Hematology/Oncology and Indiana Elks Cancer
Research Center, Department of Medicine, and Department of Pediatrics,
Herman B Wells Center for Pediatric Research, Indiana School of
Medicine, Indianapolis, IN.
Most primitive hematopoietic progenitor cells reside in vivo within
the G0/G1 phase of the cell cycle. By
simultaneous DNA/RNA staining it is possible to distinguish
G0 and G1 states and to isolate cells in
defined phases of the cell cycle. We report here the use of cell cycle
fractionation to separate human mobilized peripheral blood (MPB)
CD34+ cells capable of repopulating the bone marrow (BM)
of non-obese diabetic/severe combined immune-deficient (NOD/SCID) mice.
In freshly isolated MPB, repopulating cells were predominant within the
G0 phase, because transplantation of CD34+
cells residing in G0 (G0CD34+)
resulted on average in a 16.6- ± 3.2-fold higher BM chimerism than
infusion of equal numbers of CD34+ cells isolated in
G1. We then investigated the effect of ex vivo cell cycle
progression, in the absence of cell division, on engraftment capacity.
Freshly isolated G0CD34+ cells were activated
by interleukin-3 (IL-3), stem cell factor (SCF), and flt3-ligand (FL)
for a 36-hour incubation period during which a fraction of cells
progressed from G0 into G1 but did not complete
a cell cycle. The repopulating capacity of stimulated cells was
markedly diminished compared with that of unmanipulated G0CD34+ cells. Cells that remained in
G0 during the 36-hour incubation period and those that
traversed into G1 were sorted and assayed separately in
NOD/SCID recipients. The repopulating ability of cells remaining in
G0 was insignificantly reduced compared with that of
unstimulated G0CD34+ cells. On the contrary,
CD34+ cells traversing from G0 into
G1 were largely depleted of repopulating capacity. Similar
results were obtained when G0CD34+ cells were
activated by the combination of thrombopoietin-SCF-FL. These studies
provide direct evidence of the quiescent nature of cells capable of
repopulating the BM of NOD/SCID mice. Furthermore, these data also
demonstrate that G0-G1 progression in vitro is associated with a decrease in engraftment capacity.
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. M. Agosto, J. J. Yu, M. K. Liszewski, C. Baytop, N. Korokhov, L. M. Humeau, and U. O'Doherty
The CXCR4-Tropic Human Immunodeficiency Virus Envelope Promotes More-Efficient Gene Delivery to Resting CD4+ T Cells than the Vesicular Stomatitis Virus Glycoprotein G Envelope
J. Virol.,
August 15, 2009;
83(16):
8153 - 8162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. W. Lane, D. T. Scadden, and D. G. Gilliland
The leukemic stem cell niche: current concepts and therapeutic opportunities
Blood,
August 6, 2009;
114(6):
1150 - 1157.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. Tipping, C. Pina, A. Castor, D. Hong, N. P. Rodrigues, L. Lazzari, G. E. May, S. E. W. Jacobsen, and T. Enver
High GATA-2 expression inhibits human hematopoietic stem and progenitor cell function by effects on cell cycle
Blood,
March 19, 2009;
113(12):
2661 - 2672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Safi, G. G. Muramoto, A. B. Salter, S. Meadows, H. Himburg, L. Russell, P. Daher, P. Doan, M. D. Leibowitz, N. J. Chao, et al.
Pharmacological Manipulation of the RAR/RXR Signaling Pathway Maintains the Repopulating Capacity of Hematopoietic Stem Cells in Culture
Mol. Endocrinol.,
February 1, 2009;
23(2):
188 - 201.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Foguenne, I. Di Stefano, O. Giet, Y. Beguin, and A. Gothot
Ex vivo expansion of hematopoietic progenitor cells is associated with downregulation of {alpha}4 integrin- and CXCR4-mediated engraftment in NOD/SCID {beta}2-microglobulin-null mice
Haematologica,
February 1, 2009;
94(2):
185 - 194.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Tabatabai, C. Herrmann, G. von Kurthy, M. Mittelbronn, S. Grau, B. Frank, R. Mohle, M. Weller, and W. Wick
VEGF-dependent induction of CD62E on endothelial cells mediates glioma tropism of adult haematopoietic progenitor cells
Brain,
October 1, 2008;
131(10):
2579 - 2595.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Bonig, A. Wundes, K.-H. Chang, S. Lucas, and T. Papayannopoulou
Increased numbers of circulating hematopoietic stem/progenitor cells are chronically maintained in patients treated with the CD49d blocking antibody natalizumab
Blood,
April 1, 2008;
111(7):
3439 - 3441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Rezzoug, Y. Huang, M. K. Tanner, M. Wysoczynski, C. L. Schanie, P. M. Chilton, M. Z. Ratajczak, I. J. Fugier-Vivier, and S. T. Ildstad
TNF-{alpha} Is Critical to Facilitate Hemopoietic Stem Cell Engraftment and Function
J. Immunol.,
January 1, 2008;
180(1):
49 - 57.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Nemeth, L. Topol, S. M. Anderson, Y. Yang, and D. M. Bodine
Wnt5a inhibits canonical Wnt signaling in hematopoietic stem cells and enhances repopulation
PNAS,
September 25, 2007;
104(39):
15436 - 15441.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Fukuda, H. Bian, A. G. King, and L. M. Pelus
The chemokine GRO{beta} mobilizes early hematopoietic stem cells characterized by enhanced homing and engraftment
Blood,
August 1, 2007;
110(3):
860 - 869.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Araki, K. Yoshinaga, P. Boccuni, Y. Zhao, R. Hoffman, and N. Mahmud
Chromatin-modifying agents permit human hematopoietic stem cells to undergo multiple cell divisions while retaining their repopulating potential
Blood,
April 15, 2007;
109(8):
3570 - 3578.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Ghiaur, A. Lee, J. Bailey, J. A. Cancelas, Y. Zheng, and D. A. Williams
Inhibition of RhoA GTPase activity enhances hematopoietic stem and progenitor cell proliferation and engraftment
Blood,
September 15, 2006;
108(6):
2087 - 2094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. H. Lee, S. C. Hsu, J. Munoz, J. S. Jung, N. R. Lee, R. Pochampally, and D. J. Prockop
A subset of human rapidly self-renewing marrow stromal cells preferentially engraft in mice
Blood,
March 1, 2006;
107(5):
2153 - 2161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Verhoeyen, M. Wiznerowicz, D. Olivier, B. Izac, D. Trono, A. Dubart-Kupperschmitt, and F.-L. Cosset
Novel lentiviral vectors displaying "early-acting cytokines" selectively promote survival and transduction of NOD/SCID repopulating human hematopoietic stem cells
Blood,
November 15, 2005;
106(10):
3386 - 3395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Liang, G. Van Zant, and S. J. Szilvassy
Effects of aging on the homing and engraftment of murine hematopoietic stem and progenitor cells
Blood,
August 15, 2005;
106(4):
1479 - 1487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Li, M. M. Le Beau, S. Ciccone, F.-C. Yang, B. Freie, S. Chen, J. Yuan, P. Hong, A. Orazi, L. S. Haneline, et al.
Ex vivo culture of Fancc-/- stem/progenitor cells predisposes cells to undergo apoptosis, and surviving stem/progenitor cells display cytogenetic abnormalities and an increased risk of malignancy
Blood,
May 1, 2005;
105(9):
3465 - 3471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. F. Srour, X. Tong, K. W. Sung, P. A. Plett, S. Rice, J. Daggy, C. T. Yiannoutsos, R. Abonour, and C. M. Orschell
Modulation of in vitro proliferation kinetics and primitive hematopoietic potential of individual human CD34+CD38-/lo cells in G0
Blood,
April 15, 2005;
105(8):
3109 - 3116.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Fukuda, H. E. Broxmeyer, and L. M. Pelus
Flt3 ligand and the Flt3 receptor regulate hematopoietic cell migration by modulating the SDF-1{alpha}(CXCL12)/CXCR4 axis
Blood,
April 15, 2005;
105(8):
3117 - 3126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, G. G. Muramoto, J. Fung, and C. Oxford
Soluble factors elaborated by human brain endothelial cells induce the concomitant expansion of purified human BM CD34+CD38- cells and SCID-repopulating cells
Blood,
January 15, 2005;
105(2):
576 - 583.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. W. Christopherson II, G. Hangoc, C. R. Mantel, and H. E. Broxmeyer
Modulation of Hematopoietic Stem Cell Homing and Engraftment by CD26
Science,
August 13, 2004;
305(5686):
1000 - 1003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wagner, A. Ansorge, U. Wirkner, V. Eckstein, C. Schwager, J. Blake, K. Miesala, J. Selig, R. Saffrich, W. Ansorge, et al.
Molecular evidence for stem cell function of the slow-dividing fraction among human hematopoietic progenitor cells by genome-wide analysis
Blood,
August 1, 2004;
104(3):
675 - 686.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mazurier, O. I. Gan, J. L. McKenzie, M. Doedens, and J. E. Dick
Lentivector-mediated clonal tracking reveals intrinsic heterogeneity in the human hematopoietic stem cell compartment and culture-induced stem cell impairment
Blood,
January 15, 2004;
103(2):
545 - 552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. N. Levasseur, T. M. Ryan, K. M. Pawlik, and T. M. Townes
Correction of a mouse model of sickle cell disease: lentiviral/antisickling {beta}-globin gene transduction of unmobilized, purified hematopoietic stem cells
Blood,
December 15, 2003;
102(13):
4312 - 4319.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Kurre, J. Morris, B. Thomasson, D. B. Kohn, and H.-P. Kiem
Scaffold attachment region-containing retrovirus vectors improve long-term proviral expression after transplantation of GFP-modified CD34+ baboon repopulating cells
Blood,
November 1, 2003;
102(9):
3117 - 3119.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. N. Schwartz, B. A. Vance, B. M. Levine, M. Fukazawa, W. G. Telford, D. Cesar, M. Hellerstein, and R. E. Gress
Proliferation kinetics of subpopulations of human marrow cells determined by quantifying in vivo incorporation of [2H2]-glucose into DNA of S-phase cells
Blood,
September 15, 2003;
102(6):
2068 - 2073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Stier, T. Cheng, R. Forkert, C. Lutz, D. M. Dombkowski, J. L. Zhang, and D. T. Scadden
Ex vivo targeting of p21Cip1/Waf1 permits relative expansion of human hematopoietic stem cells
Blood,
August 15, 2003;
102(4):
1260 - 1266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Guan, B. Gerhard, and D. E. Hogge
Detection, isolation, and stimulation of quiescent primitive leukemic progenitor cells from patients with acute myeloid leukemia (AML)
Blood,
April 15, 2003;
101(8):
3142 - 3149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Laufs, B. Gentner, K. Z. Nagy, A. Jauch, A. Benner, S. Naundorf, K. Kuehlcke, B. Schiedlmeier, A. D. Ho, W. J. Zeller, et al.
Retroviral vector integration occurs in preferred genomic targets of human bone marrow-repopulating cells
Blood,
March 15, 2003;
101(6):
2191 - 2198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Hematti, S. E. Sellers, B. A. Agricola, M. E. Metzger, R. E. Donahue, and C. E. Dunbar
Retroviral transduction efficiency of G-CSF+SCF-mobilized peripheral blood CD34+ cells is superior to G-CSF or G-CSF+Flt3-L-mobilized cells in nonhuman primates
Blood,
March 15, 2003;
101(6):
2199 - 2205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. J. Quesenberry, G. A. Colvin, and J.-F. Lambert
The chiaroscuro stem cell: a unified stem cell theory
Blood,
December 15, 2002;
100(13):
4266 - 4271.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Roesler, S. Brenner, A. A. Bukovsky, N. Whiting-Theobald, T. Dull, M. Kelly, C. I. Civin, and H. L. Malech
Third-generation, self-inactivating gp91phox lentivector corrects the oxidase defect in NOD/SCID mouse-repopulating peripheral blood-mobilized CD34+ cells from patients with X-linked chronic granulomatous disease
Blood,
December 15, 2002;
100(13):
4381 - 4390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Chute, A. A. Saini, D. J. Chute, M. R. Wells, W. B. Clark, D. M. Harlan, J. Park, M. K. Stull, C. Civin, and T. A. Davis
Ex vivo culture with human brain endothelial cells increases the SCID-repopulating capacity of adult human bone marrow
Blood,
December 15, 2002;
100(13):
4433 - 4439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cashman, B. Dykstra, I. Clark-Lewis, A. Eaves, and C. Eaves
Changes in the Proliferative Activity of Human Hematopoietic Stem Cells in NOD/SCID Mice and Enhancement of Their Transplantability after In Vivo Treatment with Cell Cycle Inhibitors
J. Exp. Med.,
November 4, 2002;
196(9):
1141 - 1150.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Huygen, O. Giet, V. Artisien, I. Di Stefano, Y. Beguin, and A. Gothot
Adhesion of synchronized human hematopoietic progenitor cells to fibronectin and vascular cell adhesion molecule-1 fluctuates reversibly during cell cycle transit in ex vivo culture
Blood,
September 26, 2002;
100(8):
2744 - 2752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, M. Bhatia, H. H. H. Kanhai, R. Breese, D. K. Heilman, C. S. Johnson, J. H. F. Falkenburg, and E. F. Srour
Engraftment potential of human fetal hematopoietic cells in NOD/SCID mice is not restricted to mitotically quiescent cells
Blood,
June 17, 2002;
100(1):
120 - 127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. C. Josephson, G. Vassilopoulos, G. D. Trobridge, G. V. Priestley, B. L. Wood, T. Papayannopoulou, and D. W. Russell
Transduction of human NOD/SCID-repopulating cells with both lymphoid and myeloid potential by foamy virus vectors
PNAS,
June 11, 2002;
99(12):
8295 - 8300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm, P. Tang, I. Clark-Lewis, C. von Kalle, and C. Eaves
Ex vivo treatment of proliferating human cord blood stem cells with stroma-derived factor-1 enhances their ability to engraft NOD/SCID mice
Blood,
May 1, 2002;
99(9):
3454 - 3457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Giet, D. R. Van Bockstaele, I. Di Stefano, S. Huygen, R. Greimers, Y. Beguin, and A. Gothot
Increased binding and defective migration across fibronectin of cycling hematopoietic progenitor cells
Blood,
March 15, 2002;
99(6):
2023 - 2031.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Jetmore, P. A. Plett, X. Tong, F. M. Wolber, R. Breese, R. Abonour, C. M. Orschell-Traycoff, and E. F. Srour
Homing efficiency, cell cycle kinetics, and survival of quiescent and cycling human CD34+ cells transplanted into conditioned NOD/SCID recipients
Blood,
March 1, 2002;
99(5):
1585 - 1593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-J. Lataillade, D. Clay, P. Bourin, F. Herodin, C. Dupuy, C. Jasmin, and M.-C. Le Bousse-Kerdiles
Stromal cell-derived factor 1 regulates primitive hematopoiesis by suppressing apoptosis and by promoting G0/G1 transition in CD34+ cells: evidence for an autocrine/paracrine mechanism
Blood,
February 15, 2002;
99(4):
1117 - 1129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Dao, J. Hwa, and J. A. Nolta
Molecular mechanism of transforming growth factor beta -mediated cell-cycle modulation in primary human CD34+ progenitors
Blood,
January 15, 2002;
99(2):
499 - 506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Scherr, K. Battmer, U. Blomer, B. Schiedlmeier, A. Ganser, M. Grez, and M. Eder
Lentiviral gene transfer into peripheral blood-derived CD34+ NOD/SCID-repopulating cells
Blood,
January 15, 2002;
99(2):
709 - 712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Szilvassy, T. E. Meyerrose, P. L. Ragland, and B. Grimes
Differential homing and engraftment properties of hematopoietic progenitor cells from murine bone marrow, mobilized peripheral blood, and fetal liver
Blood,
October 1, 2001;
98(7):
2108 - 2115.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Minguell, A. Erices, and P. Conget
Mesenchymal Stem Cells
Experimental Biology and Medicine,
June 1, 2001;
226(6):
507 - 520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Perez, H. M. Rinder, C. Wang, J. B. Tracey, N. Maun, and D. S. Krause
Xenotransplantation of immunodeficient mice with mobilized human blood CD34+ cells provides an in vivo model for human megakaryocytopoiesis and platelet production
Blood,
March 15, 2001;
97(6):
1635 - 1643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Sirven, F. Pflumio, V. Zennou, M. Titeux, W. Vainchenker, L. Coulombel, A. Dubart-Kupperschmitt, and P. Charneau
The human immunodeficiency virus type-1 central DNA flap is a crucial determinant for lentiviral vector nuclear import and gene transduction of human hematopoietic stem cells
Blood,
December 15, 2000;
96(13):
4103 - 4110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm, I.-H. Oh, and C. J. Eaves
Human hematopoietic stem cells stimulated to proliferate in vitro lose engraftment potential during their S/G2/M transit and do not reenter G0
Blood,
December 15, 2000;
96(13):
4185 - 4193.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Cashman and C. J. Eaves
High marrow seeding efficiency of human lymphomyeloid repopulating cells in irradiated NOD/SCID mice
Blood,
December 1, 2000;
96(12):
3979 - 3981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Barrette, J. L. Douglas, N. E. Seidel, and D. M. Bodine
Lentivirus-based vectors transduce mouse hematopoietic stem cells with similar efficiency to Moloney murine leukemia virus-based vectors
Blood,
November 15, 2000;
96(10):
3385 - 3391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Wilpshaar, J. H. F. Falkenburg, X. Tong, W. A. Noort, R. Breese, D. Heilman, H. Kanhai, C. M. Orschell-Traycoff, and E. F. Srour
Similar repopulating capacity of mitotically active and resting umbilical cord blood CD34+ cells in NOD/SCID mice
Blood,
September 15, 2000;
96(6):
2100 - 2107.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. W. Zandstra, D. A. Lauffenburger, and C. J. Eaves
A ligand-receptor signaling threshold model of stem cell differentiation control: a biologically conserved mechanism applicable to hematopoiesis
Blood,
August 15, 2000;
96(4):
1215 - 1222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Orschell-Traycoff, K. Hiatt, R. N. Dagher, S. Rice, M. C. Yoder, and E. F. Srour
Homing and engraftment potential of Sca-1+lin- cells fractionated on the basis of adhesion molecule expression and position in cell cycle
Blood,
August 15, 2000;
96(4):
1380 - 1387.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. F. Srour;, C. Eaves, and H. Glimm
Proliferative history and hematopoietic function of ex vivo expanded human CD34+ cells
Blood,
August 15, 2000;
96(4):
1609 - 1612.
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Kim, J. F. Tisdale, T. Wu, M. Takatoku, S. E. Sellers, P. Zickler, M. E. Metzger, B. A. Agricola, J. D. Malley, I. Kato, et al.
Many multipotential gene-marked progenitor or stem cell clones contribute to hematopoiesis in nonhuman primates
Blood,
July 1, 2000;
96(1):
1 - 8.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Szilvassy, T. E. Meyerrose, and B. Grimes
Effects of cell cycle activation on the short-term engraftment properties of ex vivo expanded murine hematopoietic cells
Blood,
May 1, 2000;
95(9):
2829 - 2837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Schiedlmeier, K. Kuhlcke, H. G. Eckert, C. Baum, W. J. Zeller, and S. Fruehauf
Quantitative assessment of retroviral transfer of the human multidrug resistance 1 gene to human mobilized peripheral blood progenitor cells engrafted in nonobese diabetic/severe combined immunodeficient mice
Blood,
February 15, 2000;
95(4):
1237 - 1248.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. J. Oostendorp, J. Audet, and C. J. Eaves
High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro and in vivo
Blood,
February 1, 2000;
95(3):
855 - 862.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Williams, A. W. Nienhuis, R. G. Hawley, and F. O. Smith
Gene Therapy 2000
Hematology,
January 1, 2000;
2000(1):
376 - 393.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Glimm and C.J. Eaves
Direct Evidence for Multiple Self-Renewal Divisions of Human In Vivo Repopulating Hematopoietic Cells in Short-Term Culture
Blood,
October 1, 1999;
94(7):
2161 - 2168.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Holyoake, X. Jiang, C. Eaves, and A. Eaves
Isolation of a Highly Quiescent Subpopulation of Primitive Leukemic Cells in Chronic Myeloid Leukemia
Blood,
September 15, 1999;
94(6):
2056 - 2064.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Brandt, A. M. Bartholomew, J. D. Fortman, M. C. Nelson, E. Bruno, L. M. Chen, J. V. Turian, T. A. Davis, J. P. Chute, and R. Hoffman
Ex Vivo Expansion of Autologous Bone Marrow CD34+ Cells With Porcine Microvascular Endothelial Cells Results in a Graft Capable of Rescuing Lethally Irradiated Baboons
Blood,
July 1, 1999;
94(1):
106 - 113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. I. Rebel, M. Tanaka, J.-S. Lee, S. Hartnett, M. Pulsipher, D. G. Nathan, R. C. Mulligan, and C. A. Sieff
One-Day Ex Vivo Culture Allows Effective Gene Transfer Into Human Nonobese Diabetic/Severe Combined Immune-Deficient Repopulating Cells Using High-Titer Vesicular Stomatitis Virus G Protein Pseudotyped Retrovirus
Blood,
April 1, 1999;
93(7):
2217 - 2224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Case, M. A. Price, C. T. Jordan, X. J. Yu, L. Wang, G. Bauer, D. L. Haas, D. Xu, R. Stripecke, L. Naldini, et al.
Stable transduction of quiescent CD34+CD38- human hematopoietic cells by HIV-1-based lentiviral vectors
PNAS,
March 16, 1999;
96(6):
2988 - 2993.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
