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Blood, Vol. 92 No. 8 (October 15), 1998:
pp. 2681-2687
RAPID COMMUNICATION
Molecular and Serological Examination of the Relationship of Human
Herpesvirus 8 to Multiple Myeloma: orf 26 Sequences in Bone Marrow
Stroma Are Not Restricted to Myeloma Patients and Other Regions of
the Genome Are Not Detected
John F. Tisdale,
A. Keith Stewart,
Bruce Dickstein,
Richard F. Little,
Ian Dubé,
D. Cappe,
Cynthia E. Dunbar, and
Kevin E. Brown
From the Hematology Branch, National Heart, Lung and Blood Institute,
Bethesda, MD; The Toronto Hospital, Toronto, Ontario, Canada; HIV and
AIDS Malignancy Branch, National Cancer Institute, Bethesda, MD; and
the Department of Laboratory Medicine, Sunnybrook Regional Health
Science Center, Toronto, Ontario, Canada.
Human herpesvirus 8 (HHV-8) genomic sequences were recently detected
by polymerase chain reaction (PCR) and in situ hybridization in bone
marrow stromal cells grown from multiple myeloma (MM) patients, but not
in cells from control subjects (Rettig et al, Science 276:1851,
1997). We sought to confirm these observations in our own
group of MM patients (n = 30). DNA was extracted from adherent
stromal cells grown under varying conditions and assayed for HHV-8
sequence using PCR to amplify the orf 26 (KS330) sequence (Chang et al,
Science 266:1865, 1997), as initially reported. Samples from
human control subjects (n = 25) were concurrently extracted and
analyzed. After 30 cycles of amplification, we did not detect any
positive samples. In a more sensitive nested PCR, samples from 18 of 30 (60%) MM patients were positive, at about the limit of detection, but
orf 26 sequence was also amplified from 11 of 25 (44%) human control
samples. However, PCR amplification from other regions of the viral
genome (orf 72 and orf 75) was uniformly negative for all MM and
control samples, despite equivalent sensitivity. Additionally, all sera
from MM patients were negative for HHV-8 IgG by immunofluorescence. Our
data do not support a role of HHV-8 in the etiology of MM but may
suggest the presence of a related (KS330-containing) virus in MM
patients and in some control subjects.
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