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Blood, Vol. 92 No. 8 (October 15), 1998:
pp. 2791-2801
Tissue Distribution and Regulation of Murine von Willebrand
Factor Gene Expression In Vivo
Koji Yamamoto,
Vivian de Waard,
Colleen Fearns, and
David J. Loskutoff
From the Department of Vascular Biology (VB-3), The Scripps Research
Institute, La Jolla, CA.
von Willebrand factor (vWF) is frequently used as a biochemical
marker for endothelial cells (ECs). Despite this, little is known about
the relative level of expression and regulation of this hemostatic
factor in ECs in different vascular beds in vivo. In the present study,
we used quantitative reverse transcription polymerase chain reaction
and in situ hybridization analysis to study vWF gene expression in
murine tissues. Large differences in the level of vWF mRNA were
observed when comparing highly vascularized tissues, with the lung and
brain containing 5 to 50 times higher concentrations of vWF mRNA than
the kidney and liver. In this regard, ECs of small vessels and some
microvessels in the lung and brain expressed abundant vWF mRNA, whereas
ECs of similar sized vessels in the liver and kidney expressed
relatively low levels. In general, significantly higher levels of vWF
mRNA and antigen were demonstrated in ECs of larger vessels compared
with microvessels and in venous ECs compared with arterial ECs.
Although intraperitoneal administration of endotoxin (or tumor necrosis factor- ) increased plasma vWF levels, it had variable effects on the
steady-state level of vWF mRNA in murine tissues (ie, it decreased vWF
mRNA in many tissues, increased it in others, and had little effect on
still others). These results indicate that vWF is differentially
expressed and regulated in ECs present in different tissues and within
the same vascular bed.
© 1998 by The American Society of Hematology.
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