SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by de Andres, B. Articles by Lynch, R. G. Search for Related Content PubMed PubMed Citation Articles by de Andres, B. Articles by Lynch, R. G. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, Vol. 92 No. 8 (October 15), 1998: pp. 2823-2829 A Regulatory Role for Fc Receptors CD16 and CD32 in the Development of Murine B Cells Belen de Andres, Allan L. Mueller, Sjef Verbeek, Matyas Sandor, and Richard G. Lynch From the Department of Pathology, University of Iowa, Iowa City, IA; the Department of Immunology, University Hospital Utrecht, Utrecht, The Netherlands; and the Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI. Early in development, murine B-lineage progenitor cells express two classes of IgG Fc receptors (FcR) designated as FcRII (CD32) and FcRIII (CD16), but mature B lymphocytes only express FcRII (CD32), which functions as an inhibitor of B-cell activation when it is induced to associate with mIgM. The functions of CD16 and CD32 on B-lineage precursor cells have not previously been investigated. To search for FcR functions on developing B-lineage cells, normal murine bone marrow cells were cultured in the presence of 2.4G2, a rat monoclonal antibody that binds to CD16 and CD32, or in the presence of control normal rat IgG, and then the B-lineage compartment was analyzed for effects. Cultures that contained 2.4G2 showed enhanced growth and differentiation of B-lineage cells compared with control cultures. The enhancing effect of 2.4G2 also occurred when fluorescence-activated cell-sorted B-cell precursors (B220+, sIgM, HSAhigh, FcR+) from normal bone marrow were cocultured with BMS2, a bone marrow stromal cell line, but not when they were cultured in BMS2-conditioned media. The enhancement of B-lineage development induced by 2.4G2 was CD16-dependent and CD32-dependent, because 2.4G2 did not effect B-lineage growth or differentiation in cultures of bone marrow from mice in which either the gene encoding CD16 or CD32 had been disrupted. Analysis of fresh bone marrow from the CD16 gene-disrupted mice showed normal numbers and distribution of cells within the B-cell compartment, but in CD32 gene-disrupted mice, the B-cell compartment was significantly enlarged. These experiments provide several lines of evidence that the FcR expressed on murine B-cell precursors can influence their growth and differentiation. © 1998 by The American Society of Hematology. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. Kato, T. Takai, and A. Kudo The Pre-B Cell Receptor Signaling for Apoptosis Is Negatively Regulated by Fc{gamma}RIIB J. Immunol., January 15, 2002; 168(2): 629 - 634. [Abstract] [Full Text] [PDF] M. B. Callanan, P. Le Baccon, P. Mossuz, S. Duley, C. Bastard, R. Hamoudi, M. J. Dyer, G. Klobeck, R. Rimokh, J. J. Sotto, et al. The IgG Fc receptor, Fcgamma RIIB, is a target for deregulation by chromosomal translocation in malignant lymphoma PNAS, January 4, 2000; 97(1): 309 - 314. [Abstract] [Full Text] [PDF] E. Macintyre, D. Willerford, and S. W. Morris Non-Hodgkin's Lymphoma: Molecular Features of B Cell Lymphoma Hematology, January 1, 2000; 2000(1): 180 - 204. [Abstract] [Full Text] [PDF]

	Copyright © 1998 by American Society of Hematology         Online ISSN: 1528-0020