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Blood, Vol. 92 No. 8 (October 15), 1998:
pp. 2886-2892
Fluorescence In Situ Hybridization of Progenitor Cells Obtained
by Fluorescence-Activated Cell Sorting for the Detection of Cells
Affected by Chromosome Abnormality Trisomy 8 in Patients With
Myelodysplastic Syndromes
Kohki Saitoh,
Ikuo Miura,
Naoto Takahashi, and
Akira B. Miura
From the Third Department of Internal Medicine, Akita University
School of Medicine, Akita, Japan.
Myelodysplastic syndrome (MDS) is believed to be a stem-cell
disorder involving cytopenia and dysplastic changes in three hematopoietic lineages. However, the involvement of pluripotent stem
cells and progenitor cells has not been clarified conclusively. To
address this issue, we used fluorescence in situ hybridization (FISH)
of blood and bone marrow (BM) smears for mature cells and FISH of cells
sorted by fluorescence-activated cell sorting for progenitor cells.
Seven patients with MDS associated with trisomy 8 were studied. FISH
showed +8 in granulocytes, monocytes, and erythroblasts, but not in
lymphocytes. Sorted cells of T (CD3+), B
(CD19+), and NK cells
(CD3 CD56+) from peripheral blood did not
contain +8, nor did CD34+ subpopulations from BM
including B (CD34+CD19+), T/NK
(CD34+CD7+) progenitors, and pluripotent
stem cells (CD34+Thy1+). The +8
chromosome abnormality was identified in stem cells only at the level
of colony-forming unit of
granulocyte-erythrocyte-macrophage-megakaryocyte (CFU-GEMM;
CD34+CD33+). It may thus be concluded that
cells affected by trisomy 8 in the context of MDS are at the CFU-GEMM
level and that cells of lymphoid lineage are not involved. These
results provide new insights into the biology of MDS and suggest that
intensive chemotherapy and autologous BM transplantation may become
important therapeutic strategies.
© 1998 by The American Society of Hematology.
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