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Blood, Vol. 92 No. 9 (November 1), 1998:
pp. 3203-3209
Four-Color Flow Cytometric Investigation of Terminal Deoxynucleotidyl
Transferase-Positive Lymphoid Precursors in Pediatric Bone Marrow:
CD79a Expression Precedes CD19 in Early B-Cell Ontogeny
Michael N. Dworzak,
Gerhard Fritsch,
Gertraud Fröschl,
Dieter Printz, and
Helmut Gadner
From the Children's Cancer Research Institute, St Anna Kinderspital,
Vienna, Austria.
Terminal deoxynucleotidyl transferase (TdT)-positive cells in human
bone marrow (BM) are a phenotypically inhomogeneous population of
precursor cells. In their majority, these TdT+ cells are
unambiguously committed to the B lineage, as evidenced by CD19
expression. However, TdT+ precursors that lack CD19 also
exist and these may encompass a differentiation potential for the B as
well as for other lineages. Because recent data suggested that CD19
expression is not the earliest differentiation event in B-cell
ontogeny, we sought to reevaluate TdT+ lymphoid
precursors in pediatric BM to define the phenotypic denominator of
B-lineage affiliation upstream of CD19. Using four-color flow
cytometry, we focused on the assessment of the CD79a antigen, which is
highly B-cell specific and which may also be expressed very early in
B-cell ontogeny. We found that a majority of TdT+ cells
coexpressed CD19 and CD79a in addition to CD10 and CD34, whereas, in
all investigated samples, some TdT+ precursors lacked
CD19 but expressed CD79a, which suggestively indicates also their
B-lineage affiliation. In contrast to the CD19+
precursors, which were usually CD10hi and
CD79b+, these CD19 CD79a+
putative B-cell precursors preferentially expressed CD10 at low levels
and were CD79b+ in only 41%. About 17% of these
TdT+CD19CD79a+ precursors
also coexpressed CD33 and CD7, but not myeloperoxidase, CD14, or
cytoplasmic CD3, which is discussed in the light of cellular activation
rather than lineage promiscuity. Our data confirm that the earliest
differentiation stages of B cells can be dissected upon expression of
the lineage antigens CD79a and CD19 and imply that CD79a is earlier
expressed than CD19. This raises the chance to follow the sequential
events heralding B-cell commitment in the most immature precursors by
correlating phenotypic and genetic differentiation markers.
© 1998 by The American Society of Hematology.
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