|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 92 No. 9 (November 1), 1998:
pp. 3416-3421
Comparison of Expression of Human Globin Genes Transferred Into Mouse
Erythroleukemia Cells and in Transgenic Mice
E. Skarpidi,
G. Vassilopoulos,
G. Stamatoyannopoulos, and
Q. Li
From the Division of Medical Genetics, University of Washington,
Seattle, WA.
To examine whether transfer of  globin genes into mouse
erythroleukemia cells can be used for the analysis of regulatory elements of globin gene promoter, A gene constructs
carrying promoter truncations that have been previously analyzed in
transgenic mice were used for production of stably transfected mouse
erythroleukemia (MEL) cell clones and pools. We found that constructs,
which contain a microlocus control region (µLCR) that efficiently
protects globin gene expression from the effects of the position of
integration in transgenic mice, display position-dependent globin gene
expression in MEL cell clones. A globin gene expression
among MEL cell clones carrying the µLCR( 201)A and
µLCR(382)A gene constructs ranged 15.5-fold and
17.6-fold, respectively, and there was no correlation between the
A mRNA levels and the copies of the transgene (r
= .28, P = .18). There was significant variation in per
copy A globin gene expression among MEL cell pools
composed of 10 clones, but not among pools composed of 50 clones,
indicating that position effects are averaged in pools composed by
large numbers of clones. The overall pattern of A globin
gene expression in MEL cell pools resembled that observed in transgenic
mice indicating that MEL cell transfections can be used in the study of
cis elements controlling globin gene expression. MEL cell transfections, however, are not appropriate for
investigation of cis elements, which either sensitize or
protect the globin transgenes from position effects.
© 1998 by The American Society of Hematology.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Lisowski and M. Sadelain
Locus control region elements HS1 and HS4 enhance the therapeutic efficacy of globin gene transfer in -thalassemic mice
Blood,
December 15, 2007;
110(13):
4175 - 4178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Gomos-Klein, F. Harrow, J. Alarcon, and B. D. Ortiz
CTCF-Independent, but Not CTCF-Dependent, Elements Significantly Contribute to TCR-{alpha} Locus Control Region Activity
J. Immunol.,
July 15, 2007;
179(2):
1088 - 1095.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Blau, C. F. Barbas III, A. L. Bomhoff, R. Neades, J. Yan, P. A. Navas, and K. R. Peterson
{gamma}-Globin Gene Expression in Chemical Inducer of Dimerization (CID)-dependent Multipotential Cells Established from Human {beta}-Globin Locus Yeast Artificial Chromosome ({beta}-YAC) Transgenic Mice
J. Biol. Chem.,
November 4, 2005;
280(44):
36642 - 36647.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. J. Dempsey, L. S. Ojalvo, D. W. Wu, and J. A. Little
Induction of an embryonic globin gene promoter by short-chain fatty acids
Blood,
December 1, 2003;
102(12):
4214 - 4222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. W. Emery, E. Yannaki, J. Tubb, T. Nishino, Q. Li, and G. Stamatoyannopoulos
Development of virus vectors for gene therapy of beta chain hemoglobinopathies: flanking with a chromatin insulator reduces gamma -globin gene silencing in vivo
Blood,
August 28, 2002;
100(6):
2012 - 2019.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Duan, G. Stamatoyannopoulos, and Q. Li
Role of NF-Y in In Vivo Regulation of the {gamma}-Globin Gene
Mol. Cell. Biol.,
May 1, 2001;
21(9):
3083 - 3095.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Rivella, J. A. Callegari, C. May, C. W. Tan, and M. Sadelain
The cHS4 Insulator Increases the Probability of Retroviral Expression at Random Chromosomal Integration Sites
J. Virol.,
May 15, 2000;
74(10):
4679 - 4687.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Q. Li, D. W. Emery, M. Fernandez, H. Han, and G. Stamatoyannopoulos
Development of Viral Vectors for Gene Therapy of beta -Chain Hemoglobinopathies: Optimization of a gamma -Globin Gene Expression Cassette
Blood,
April 1, 1999;
93(7):
2208 - 2216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
